Thymoquinone (Tq) protects necroptosis induced by autophagy/mitophagy‐dependent oxidative stress in human bronchial epithelial cells exposed to cigarette smoke extract (CSE).
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| Title: | Thymoquinone (Tq) protects necroptosis induced by autophagy/mitophagy‐dependent oxidative stress in human bronchial epithelial cells exposed to cigarette smoke extract (CSE). |
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| Authors: | Dera, Ayed A.1,2 (AUTHOR), Al Fayi, Majed1,2 (AUTHOR), Otifi, Hassan3 (AUTHOR), Alshyarba, Mishari4 (AUTHOR), Alfhili, Mohammad5 (AUTHOR), Rajagopalan, Prasanna1,2 (AUTHOR) prachu.rg@gmail.com |
| Source: | Journal of Food Biochemistry. Sep2020, Vol. 44 Issue 9, p1-11. 11p. |
| Subjects: | Cigarette smoke, Epithelial cells, Obstructive lung diseases, Oxidative stress, Black cumin, Mitochondrial membranes |
| Abstract: | Chronic obstructive pulmonary disease (COPD) is characterized by cigarette smoke‐induced emphysema. Herein, we demonstrate protective effects of Thymoquinone (Tq), an active constituent from Nigella sativa, against cigarette smoke extract (CSE)‐induced abnormalities in bronchial epithelial cells. Dose‐dependent reduction in cell viability was observed in BEAS‐2B cells when exposed to different CSE concentrations, which was significantly reversed by Tq evident by LDH release. Levels of SOD, CAT, GR, GSH, and mitochondrial membrane ATPases were significantly reduced upon CSE exposure, an event, again, antagonized in presence of Tq. Similarly, Tq treatment significantly blocked CSE‐induced 4HNE elevations. Further, Tq‐improved mitochondrial dysfunction caused by CSE and significantly decreased autophagy/mitophagy markers like LC3II and p‐Drp. Tq also reduced necroptosis markers such as p‐MLKL, RIP‐1, and RIP‐3, by stabilizing PINK‐1 levels. In summary, Tq possesses protective properties against human bronchial epithelial cell autophagy/mitophagy‐dependent necroptosis caused by CSE, which warrants considerable attention for further preclinical evaluations. Practical applications: This study demonstrates Thymoquinone (Tq), a natural plant extract to possess protective properties against human bronchial epithelial cell autophagy/mitophagy‐dependent necroptosis caused by cigarette smoke extract. The demonstrated efficacy of Tq will throw light for further preclinical evaluation of this molecule in CSE‐mediated complications. A detailed in vivo research is recommended. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Food Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 145718186 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Thymoquinone (Tq) protects necroptosis induced by autophagy/mitophagy‐dependent oxidative stress in human bronchial epithelial cells exposed to cigarette smoke extract (CSE). – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Dera%2C+Ayed+A%2E%22">Dera, Ayed A.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Al+Fayi%2C+Majed%22">Al Fayi, Majed</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Otifi%2C+Hassan%22">Otifi, Hassan</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Alshyarba%2C+Mishari%22">Alshyarba, Mishari</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Alfhili%2C+Mohammad%22">Alfhili, Mohammad</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Rajagopalan%2C+Prasanna%22">Rajagopalan, Prasanna</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> prachu.rg@gmail.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Food+Biochemistry%22">Journal of Food Biochemistry</searchLink>. Sep2020, Vol. 44 Issue 9, p1-11. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Cigarette+smoke%22">Cigarette smoke</searchLink><br /><searchLink fieldCode="DE" term="%22Epithelial+cells%22">Epithelial cells</searchLink><br /><searchLink fieldCode="DE" term="%22Obstructive+lung+diseases%22">Obstructive lung diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Oxidative+stress%22">Oxidative stress</searchLink><br /><searchLink fieldCode="DE" term="%22Black+cumin%22">Black cumin</searchLink><br /><searchLink fieldCode="DE" term="%22Mitochondrial+membranes%22">Mitochondrial membranes</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Chronic obstructive pulmonary disease (COPD) is characterized by cigarette smoke‐induced emphysema. Herein, we demonstrate protective effects of Thymoquinone (Tq), an active constituent from Nigella sativa, against cigarette smoke extract (CSE)‐induced abnormalities in bronchial epithelial cells. Dose‐dependent reduction in cell viability was observed in BEAS‐2B cells when exposed to different CSE concentrations, which was significantly reversed by Tq evident by LDH release. Levels of SOD, CAT, GR, GSH, and mitochondrial membrane ATPases were significantly reduced upon CSE exposure, an event, again, antagonized in presence of Tq. Similarly, Tq treatment significantly blocked CSE‐induced 4HNE elevations. Further, Tq‐improved mitochondrial dysfunction caused by CSE and significantly decreased autophagy/mitophagy markers like LC3II and p‐Drp. Tq also reduced necroptosis markers such as p‐MLKL, RIP‐1, and RIP‐3, by stabilizing PINK‐1 levels. In summary, Tq possesses protective properties against human bronchial epithelial cell autophagy/mitophagy‐dependent necroptosis caused by CSE, which warrants considerable attention for further preclinical evaluations. Practical applications: This study demonstrates Thymoquinone (Tq), a natural plant extract to possess protective properties against human bronchial epithelial cell autophagy/mitophagy‐dependent necroptosis caused by cigarette smoke extract. The demonstrated efficacy of Tq will throw light for further preclinical evaluation of this molecule in CSE‐mediated complications. A detailed in vivo research is recommended. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Food Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/jfbc.13366 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 1 Subjects: – SubjectFull: Cigarette smoke Type: general – SubjectFull: Epithelial cells Type: general – SubjectFull: Obstructive lung diseases Type: general – SubjectFull: Oxidative stress Type: general – SubjectFull: Black cumin Type: general – SubjectFull: Mitochondrial membranes Type: general Titles: – TitleFull: Thymoquinone (Tq) protects necroptosis induced by autophagy/mitophagy‐dependent oxidative stress in human bronchial epithelial cells exposed to cigarette smoke extract (CSE). Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Dera, Ayed A. – PersonEntity: Name: NameFull: Al Fayi, Majed – PersonEntity: Name: NameFull: Otifi, Hassan – PersonEntity: Name: NameFull: Alshyarba, Mishari – PersonEntity: Name: NameFull: Alfhili, Mohammad – PersonEntity: Name: NameFull: Rajagopalan, Prasanna IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: Sep2020 Type: published Y: 2020 Identifiers: – Type: issn-print Value: 01458884 Numbering: – Type: volume Value: 44 – Type: issue Value: 9 Titles: – TitleFull: Journal of Food Biochemistry Type: main |
| ResultId | 1 |
