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Separation of low-molecular mass peptides by capillary electrophoresis with the use of alkylamines as dynamic coating agents at low pH

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Title: Separation of low-molecular mass peptides by capillary electrophoresis with the use of alkylamines as dynamic coating agents at low pH
Authors: Eckhardt, Adam1, Mikšík, Ivan miksik@biomed.cas.cz, Deyl, Zdeněk2, Charvátová, Jana2
Source: Journal of Chromatography A. Oct2004, Vol. 1051 Issue 1/2, p111-117. 7p.
Subjects: Separation (Technology), Peptides, Proteins, Capillary electrophoresis
Abstract: Modification of the silanophilic activity of the inner surface of the capillary wall was studied in a capillary electrophoretic system using alkylamines containing background electrolytes at acid pH. The effect of the following amine additives was investigated:alkyl-α,ω-diamines (1,2-diaminoethane, 1,4-diaminobutane, 1,7-diaminoheptane, spermine),polymeric amines (polyethylenimine, polybrene),cationic amine surfactants (cetrimide, hexamethonium bromide).A seven membered test mixture of peptides (Gly–Pro–Ala, Pro–hPro, Gly–Pro–Arg, Gly–Pro–Gln, Lys–Pro–Gly, Asn–Pro–Gly, His–Pro–Gly) possessing one or more amino groups was used for selectivity evaluation. Under optimised concentration of the amine modifiers the selectivity was always improved (except for polybrene), particularly with the fast moving analytes. The best results were obtained with 1,2-diaminoethane and 1,7-diaminoheptane. On the other hand with slowly moving peaks the best separations were obtained with 1,7-diaminoheptane, hexadecyltrimethylammonium bromide and hexamethonium bromide, i.e. with modifiers possessing large aliphatic domains which are likely to be hydrophobically bonded with the separated solutes. The selectivity improvement with fast moving members of the test mixture can be ascribed to the decrease of the electroosmotic flow, while the improved separation with slowly moving peaks appears to reflect the altered interaction with the hydrophobized capillary wall. As expected the endoosmotic flow was in all cases decreased. The practical applicability of using amine based dynamic modifiers of the capillary wall was demonstrated on a natural peptide mixture (bacterial collagenase hydrolysate of collagen types I and III). [Copyright &y& Elsevier]
Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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An: 14580416
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  Data: Separation of low-molecular mass peptides by capillary electrophoresis with the use of alkylamines as dynamic coating agents at low pH
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  Data: <searchLink fieldCode="AR" term="%22Eckhardt%2C+Adam%22">Eckhardt, Adam</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Mikšík%2C+Ivan%22">Mikšík, Ivan</searchLink><i> miksik@biomed.cas.cz</i><br /><searchLink fieldCode="AR" term="%22Deyl%2C+Zdeněk%22">Deyl, Zdeněk</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Charvátová%2C+Jana%22">Charvátová, Jana</searchLink><relatesTo>2</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+A%22">Journal of Chromatography A</searchLink>. Oct2004, Vol. 1051 Issue 1/2, p111-117. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Separation+%28Technology%29%22">Separation (Technology)</searchLink><br /><searchLink fieldCode="DE" term="%22Peptides%22">Peptides</searchLink><br /><searchLink fieldCode="DE" term="%22Proteins%22">Proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Capillary+electrophoresis%22">Capillary electrophoresis</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Modification of the silanophilic activity of the inner surface of the capillary wall was studied in a capillary electrophoretic system using alkylamines containing background electrolytes at acid pH. The effect of the following amine additives was investigated:<ce:list><ce:list-item>alkyl-α,ω-diamines (1,2-diaminoethane, 1,4-diaminobutane, 1,7-diaminoheptane, spermine),</ce:list-item><ce:list-item>polymeric amines (polyethylenimine, polybrene),</ce:list-item><ce:list-item>cationic amine surfactants (cetrimide, hexamethonium bromide).</ce:list-item></ce:list>A seven membered test mixture of peptides (Gly–Pro–Ala, Pro–hPro, Gly–Pro–Arg, Gly–Pro–Gln, Lys–Pro–Gly, Asn–Pro–Gly, His–Pro–Gly) possessing one or more amino groups was used for selectivity evaluation. Under optimised concentration of the amine modifiers the selectivity was always improved (except for polybrene), particularly with the fast moving analytes. The best results were obtained with 1,2-diaminoethane and 1,7-diaminoheptane. On the other hand with slowly moving peaks the best separations were obtained with 1,7-diaminoheptane, hexadecyltrimethylammonium bromide and hexamethonium bromide, i.e. with modifiers possessing large aliphatic domains which are likely to be hydrophobically bonded with the separated solutes. The selectivity improvement with fast moving members of the test mixture can be ascribed to the decrease of the electroosmotic flow, while the improved separation with slowly moving peaks appears to reflect the altered interaction with the hydrophobized capillary wall. As expected the endoosmotic flow was in all cases decreased. The practical applicability of using amine based dynamic modifiers of the capillary wall was demonstrated on a natural peptide mixture (bacterial collagenase hydrolysate of collagen types I and III). [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.chroma.2004.07.040
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      – Code: eng
        Text: English
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        PageCount: 7
        StartPage: 111
    Subjects:
      – SubjectFull: Separation (Technology)
        Type: general
      – SubjectFull: Peptides
        Type: general
      – SubjectFull: Proteins
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      – SubjectFull: Capillary electrophoresis
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    Titles:
      – TitleFull: Separation of low-molecular mass peptides by capillary electrophoresis with the use of alkylamines as dynamic coating agents at low pH
        Type: main
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            NameFull: Eckhardt, Adam
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            NameFull: Mikšík, Ivan
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            NameFull: Deyl, Zdeněk
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            NameFull: Charvátová, Jana
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            – D: 08
              M: 10
              Text: Oct2004
              Type: published
              Y: 2004
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            – TitleFull: Journal of Chromatography A
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