View in EDS

Homo-oligomerization Is the Essential Function of the Tandem BRCT Domains in the Checkpoint Protein Crb2.

Saved in:
Bibliographic Details
Title: Homo-oligomerization Is the Essential Function of the Tandem BRCT Domains in the Checkpoint Protein Crb2.
Authors: Li-Lin Du1, Moser, Bettina A.1, Russell, Paul1 prussell@scripps.edu
Source: Journal of Biological Chemistry. 9/10/2004, Vol. 279 Issue 37, p38409-38414. 6p. 30 Black and White Photographs, 1 Chart, 3 Graphs.
Subjects: Proteins, DNA damage, Genes, Nucleic acids, Genetic mutation, Biochemical genetics, Saccharomyces cerevisiae
Abstract: BRCT (BRCA1 C terminus) domains are frequently found as a tandem repeat in proteins involved in DNA damage responses, such as Saccharomyces cerevisiae Rad9, human 53BP1 and BRCA1. Tandem BRCT domains mediate protein-protein and protein-DNA interactions. However, the functional significance of these interactions is largely unknown. Here we report the oligomerization of Schizosaccharomyces pombe checkpoint protein Crb2 through its tandem BRCT domains. Truncated Crb2 without BRCT domains is defective in DNA damage checkpoint signaling. However, addition of either of two heterologous dimerization motifs largely restores the functions of truncated Crb2 without BRCT domains. Replacement of Crb2 BRCT domains with a dimerization motif also renders cells resistant to the dominant negative effect of overexpressing Crb2 BRCT domains. These results demonstrate that the crucial function of the tandem BRCT domains is to oligomerize Crb2. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 14601893
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Homo-oligomerization Is the Essential Function of the Tandem BRCT Domains in the Checkpoint Protein Crb2.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Li-Lin+Du%22">Li-Lin Du</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Moser%2C+Bettina+A%2E%22">Moser, Bettina A.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Russell%2C+Paul%22">Russell, Paul</searchLink><relatesTo>1</relatesTo><i> prussell@scripps.edu</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Journal+of+Biological+Chemistry%22">Journal of Biological Chemistry</searchLink>. 9/10/2004, Vol. 279 Issue 37, p38409-38414. 6p. 30 Black and White Photographs, 1 Chart, 3 Graphs.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Proteins%22">Proteins</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+damage%22">DNA damage</searchLink><br /><searchLink fieldCode="DE" term="%22Genes%22">Genes</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleic+acids%22">Nucleic acids</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+mutation%22">Genetic mutation</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemical+genetics%22">Biochemical genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Saccharomyces+cerevisiae%22">Saccharomyces cerevisiae</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: BRCT (BRCA1 C terminus) domains are frequently found as a tandem repeat in proteins involved in DNA damage responses, such as Saccharomyces cerevisiae Rad9, human 53BP1 and BRCA1. Tandem BRCT domains mediate protein-protein and protein-DNA interactions. However, the functional significance of these interactions is largely unknown. Here we report the oligomerization of Schizosaccharomyces pombe checkpoint protein Crb2 through its tandem BRCT domains. Truncated Crb2 without BRCT domains is defective in DNA damage checkpoint signaling. However, addition of either of two heterologous dimerization motifs largely restores the functions of truncated Crb2 without BRCT domains. Replacement of Crb2 BRCT domains with a dimerization motif also renders cells resistant to the dominant negative effect of overexpressing Crb2 BRCT domains. These results demonstrate that the crucial function of the tandem BRCT domains is to oligomerize Crb2. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=14601893
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1074/jbc.M403326200
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 6
        StartPage: 38409
    Subjects:
      – SubjectFull: Proteins
        Type: general
      – SubjectFull: DNA damage
        Type: general
      – SubjectFull: Genes
        Type: general
      – SubjectFull: Nucleic acids
        Type: general
      – SubjectFull: Genetic mutation
        Type: general
      – SubjectFull: Biochemical genetics
        Type: general
      – SubjectFull: Saccharomyces cerevisiae
        Type: general
    Titles:
      – TitleFull: Homo-oligomerization Is the Essential Function of the Tandem BRCT Domains in the Checkpoint Protein Crb2.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Li-Lin Du
      – PersonEntity:
          Name:
            NameFull: Moser, Bettina A.
      – PersonEntity:
          Name:
            NameFull: Russell, Paul
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 10
              M: 09
              Text: 9/10/2004
              Type: published
              Y: 2004
          Identifiers:
            – Type: issn-print
              Value: 00219258
          Numbering:
            – Type: volume
              Value: 279
            – Type: issue
              Value: 37
          Titles:
            – TitleFull: Journal of Biological Chemistry
              Type: main
ResultId 1