Dissection and physical mapping of wheat chromosome 7B by inducing meiotic recombination with its homoeologues in Aegilops speltoides and Thinopyrum elongatum.
Saved in:
| Title: | Dissection and physical mapping of wheat chromosome 7B by inducing meiotic recombination with its homoeologues in Aegilops speltoides and Thinopyrum elongatum. |
|---|---|
| Authors: | Zhang, Mingyi1 (AUTHOR), Zhang, Wei1 (AUTHOR), Zhu, Xianwen1 (AUTHOR), Sun, Qing2 (AUTHOR), Yan, Changhui2 (AUTHOR), Xu, Steven S.3 (AUTHOR), Fiedler, Jason3 (AUTHOR), Cai, Xiwen1 (AUTHOR) xiwen.cai@ndsu.edu |
| Source: | Theoretical & Applied Genetics. Dec2020, Vol. 133 Issue 12, p3455-3467. 13p. |
| Subjects: | Gene mapping, Wheat breeding, Fluorescence in situ hybridization, Aegilops, Chromosome abnormalities, Introgression (Genetics), Wheat diseases & pests, Genetic markers |
| Abstract: | Key message: We constructed a homoeologous recombination-based bin map of wheat chromosome 7B, providing a unique physical framework for further study of chromosome 7B and its homoeologues in wheat and its relatives. Homoeologous recombination leads to the dissection and diversification of the wheat genome. Advances in genome sequencing and genotyping have dramatically improved the efficacy and throughput of homoeologous recombination-based genome studies and alien introgression in wheat and its relatives. In this study, we aimed to physically dissect and map wheat chromosome 7B by inducing meiotic recombination of chromosome 7B with its homoeologues 7E in Thinopyrum elongatum and 7S in Aegilops speltoides. The special genotypes, which were double monosomic for chromosomes 7B' + 7E' or 7B' + 7S' and homozygous for the ph1b mutant, were produced to enhance 7B − 7E and 7B − 7S recombination. Chromosome-specific DNA markers were developed and used to pre-screen the large recombination populations for 7B − 7E and 7B − 7S recombinants. The DNA marker-mediated preselections were verified by fluorescent genomic in situ hybridization (GISH). In total, 29 7B − 7E and 61 7B − 7S recombinants and multiple chromosome aberrations were recovered and delineated by GISH and the wheat 90 K SNP assay. Integrated GISH and SNP analysis of the recombinants physically mapped the recombination breakpoints and partitioned wheat chromosome 7B into 44 bins with 523 SNPs assigned within. A composite bin map was constructed for chromosome 7B, showing the bin size and physical distribution of SNPs. This provides a unique physical framework for further study of chromosome 7B and its homoeologues. In addition, the 7B − 7E and 7B − 7S recombinants extend the genetic variability of wheat chromosome 7B and represent useful germplasm for wheat breeding. Thereby, this genomics-enabled chromosome engineering approach facilitates wheat genome study and enriches the gene pool of wheat improvement. [ABSTRACT FROM AUTHOR] |
| Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
|
Full text is not displayed to guests.
Login for full access.
|
|
| FullText | Links: – Type: pdflink Text: Availability: 1 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 146476078 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Dissection and physical mapping of wheat chromosome 7B by inducing meiotic recombination with its homoeologues in Aegilops speltoides and Thinopyrum elongatum. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Zhang%2C+Mingyi%22">Zhang, Mingyi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Wei%22">Zhang, Wei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhu%2C+Xianwen%22">Zhu, Xianwen</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sun%2C+Qing%22">Sun, Qing</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yan%2C+Changhui%22">Yan, Changhui</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xu%2C+Steven+S%2E%22">Xu, Steven S.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Fiedler%2C+Jason%22">Fiedler, Jason</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cai%2C+Xiwen%22">Cai, Xiwen</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> xiwen.cai@ndsu.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. Dec2020, Vol. 133 Issue 12, p3455-3467. 13p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Gene+mapping%22">Gene mapping</searchLink><br /><searchLink fieldCode="DE" term="%22Wheat+breeding%22">Wheat breeding</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorescence+in+situ+hybridization%22">Fluorescence in situ hybridization</searchLink><br /><searchLink fieldCode="DE" term="%22Aegilops%22">Aegilops</searchLink><br /><searchLink fieldCode="DE" term="%22Chromosome+abnormalities%22">Chromosome abnormalities</searchLink><br /><searchLink fieldCode="DE" term="%22Introgression+%28Genetics%29%22">Introgression (Genetics)</searchLink><br /><searchLink fieldCode="DE" term="%22Wheat+diseases+%26+pests%22">Wheat diseases & pests</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+markers%22">Genetic markers</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Key message: We constructed a homoeologous recombination-based bin map of wheat chromosome 7B, providing a unique physical framework for further study of chromosome 7B and its homoeologues in wheat and its relatives. Homoeologous recombination leads to the dissection and diversification of the wheat genome. Advances in genome sequencing and genotyping have dramatically improved the efficacy and throughput of homoeologous recombination-based genome studies and alien introgression in wheat and its relatives. In this study, we aimed to physically dissect and map wheat chromosome 7B by inducing meiotic recombination of chromosome 7B with its homoeologues 7E in Thinopyrum elongatum and 7S in Aegilops speltoides. The special genotypes, which were double monosomic for chromosomes 7B' + 7E' or 7B' + 7S' and homozygous for the ph1b mutant, were produced to enhance 7B − 7E and 7B − 7S recombination. Chromosome-specific DNA markers were developed and used to pre-screen the large recombination populations for 7B − 7E and 7B − 7S recombinants. The DNA marker-mediated preselections were verified by fluorescent genomic in situ hybridization (GISH). In total, 29 7B − 7E and 61 7B − 7S recombinants and multiple chromosome aberrations were recovered and delineated by GISH and the wheat 90 K SNP assay. Integrated GISH and SNP analysis of the recombinants physically mapped the recombination breakpoints and partitioned wheat chromosome 7B into 44 bins with 523 SNPs assigned within. A composite bin map was constructed for chromosome 7B, showing the bin size and physical distribution of SNPs. This provides a unique physical framework for further study of chromosome 7B and its homoeologues. In addition, the 7B − 7E and 7B − 7S recombinants extend the genetic variability of wheat chromosome 7B and represent useful germplasm for wheat breeding. Thereby, this genomics-enabled chromosome engineering approach facilitates wheat genome study and enriches the gene pool of wheat improvement. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=146476078 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00122-020-03680-3 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 13 StartPage: 3455 Subjects: – SubjectFull: Gene mapping Type: general – SubjectFull: Wheat breeding Type: general – SubjectFull: Fluorescence in situ hybridization Type: general – SubjectFull: Aegilops Type: general – SubjectFull: Chromosome abnormalities Type: general – SubjectFull: Introgression (Genetics) Type: general – SubjectFull: Wheat diseases & pests Type: general – SubjectFull: Genetic markers Type: general Titles: – TitleFull: Dissection and physical mapping of wheat chromosome 7B by inducing meiotic recombination with its homoeologues in Aegilops speltoides and Thinopyrum elongatum. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Zhang, Mingyi – PersonEntity: Name: NameFull: Zhang, Wei – PersonEntity: Name: NameFull: Zhu, Xianwen – PersonEntity: Name: NameFull: Sun, Qing – PersonEntity: Name: NameFull: Yan, Changhui – PersonEntity: Name: NameFull: Xu, Steven S. – PersonEntity: Name: NameFull: Fiedler, Jason – PersonEntity: Name: NameFull: Cai, Xiwen IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 12 Text: Dec2020 Type: published Y: 2020 Identifiers: – Type: issn-print Value: 00405752 Numbering: – Type: volume Value: 133 – Type: issue Value: 12 Titles: – TitleFull: Theoretical & Applied Genetics Type: main |
| ResultId | 1 |
