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Transcriptional analysis of metabolic and virulence genes associated with biofilm formation in Piscirickettsia salmonis strains.

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Title: Transcriptional analysis of metabolic and virulence genes associated with biofilm formation in Piscirickettsia salmonis strains.
Authors: Zúñiga, A1 (AUTHOR), Solis, C1 (AUTHOR), Cartes, C1,2 (AUTHOR), Nourdin, G2 (AUTHOR), Yañez, A1,2 (AUTHOR), Romero, A2,3 (AUTHOR), Haussmann, D4 (AUTHOR), Figueroa, J1,2 (AUTHOR) jefigueroa@uach.cl
Source: FEMS Microbiology Letters. Nov2020, Vol. 367 Issue 21, p1-9. 9p.
Subjects: Salmonella typhimurium, Genes, Pseudomonas aeruginosa infections, Gene expression, Glutamine, Biofilms
Geographic Terms: Chile
Abstract: Piscirickettsia salmonis is a facultative intracellular bacterium that generates piscirickettsiosis affecting salmonids in Chile. The bacterium has the adaptability to survive in the marine environment under multiple stressful conditions. In this sense, this work focused on the analysis of a gene battery associated with biofilm formation under different culture conditions and on the adaptability of this biofilm to different media. The results indicated that the strains LF-89, IBM-034 and IBM-040 were strong biofilm producers, evidencing adaptability to the media by increasing the amount of biofilm through successive growths. Transcript levels of six genes described in various bacteria and P. salmonis , considered to have metabolic functions, and playing a relevant role in biofilm formation, were analyzed to evaluate bacterial functionality in the biofilm. The genes mazE-mazF , implicated in biofilm and stress, were markedly overexpressed in the biofilm condition in the three strains. For its part, gene gltA , an indicator of metabolic activity and related to virulence inhibition in Salmonella typhimurium , also seems to restrain the pathogenesis process in P. salmonis by inhibiting the expression of the virulence-associated genes liso and tcf. Finally, the expression of the glnA gene suggests the use of glutamine as an essential element for the growth of the biofilm. [ABSTRACT FROM AUTHOR]
Copyright of FEMS Microbiology Letters is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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  Label: Title
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  Data: Transcriptional analysis of metabolic and virulence genes associated with biofilm formation in Piscirickettsia salmonis strains.
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  Data: <searchLink fieldCode="AR" term="%22Zúñiga%2C+A%22">Zúñiga, A</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Solis%2C+C%22">Solis, C</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cartes%2C+C%22">Cartes, C</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Nourdin%2C+G%22">Nourdin, G</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yañez%2C+A%22">Yañez, A</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Romero%2C+A%22">Romero, A</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Haussmann%2C+D%22">Haussmann, D</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Figueroa%2C+J%22">Figueroa, J</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> jefigueroa@uach.cl</i>
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  Data: <searchLink fieldCode="JN" term="%22FEMS+Microbiology+Letters%22">FEMS Microbiology Letters</searchLink>. Nov2020, Vol. 367 Issue 21, p1-9. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Salmonella+typhimurium%22">Salmonella typhimurium</searchLink><br /><searchLink fieldCode="DE" term="%22Genes%22">Genes</searchLink><br /><searchLink fieldCode="DE" term="%22Pseudomonas+aeruginosa+infections%22">Pseudomonas aeruginosa infections</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Glutamine%22">Glutamine</searchLink><br /><searchLink fieldCode="DE" term="%22Biofilms%22">Biofilms</searchLink>
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  Data: <searchLink fieldCode="DE" term="%22Chile%22">Chile</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Piscirickettsia salmonis is a facultative intracellular bacterium that generates piscirickettsiosis affecting salmonids in Chile. The bacterium has the adaptability to survive in the marine environment under multiple stressful conditions. In this sense, this work focused on the analysis of a gene battery associated with biofilm formation under different culture conditions and on the adaptability of this biofilm to different media. The results indicated that the strains LF-89, IBM-034 and IBM-040 were strong biofilm producers, evidencing adaptability to the media by increasing the amount of biofilm through successive growths. Transcript levels of six genes described in various bacteria and P. salmonis , considered to have metabolic functions, and playing a relevant role in biofilm formation, were analyzed to evaluate bacterial functionality in the biofilm. The genes mazE-mazF , implicated in biofilm and stress, were markedly overexpressed in the biofilm condition in the three strains. For its part, gene gltA , an indicator of metabolic activity and related to virulence inhibition in Salmonella typhimurium , also seems to restrain the pathogenesis process in P. salmonis by inhibiting the expression of the virulence-associated genes liso and tcf. Finally, the expression of the glnA gene suggests the use of glutamine as an essential element for the growth of the biofilm. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of FEMS Microbiology Letters is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1093/femsle/fnaa180
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        Text: English
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        Type: general
      – SubjectFull: Genes
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      – SubjectFull: Pseudomonas aeruginosa infections
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      – SubjectFull: Glutamine
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      – SubjectFull: Biofilms
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      – SubjectFull: Chile
        Type: general
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      – TitleFull: Transcriptional analysis of metabolic and virulence genes associated with biofilm formation in Piscirickettsia salmonis strains.
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              M: 11
              Text: Nov2020
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