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Studies on binding of single‐stranded DNA with reduced graphene oxide–silver nanocomposites.

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Title: Studies on binding of single‐stranded DNA with reduced graphene oxide–silver nanocomposites.
Authors: Li, Xi1 (AUTHOR), Yang, Linqing2 (AUTHOR), Wang, Yunfei2 (AUTHOR), Du, Zhongyu3 (AUTHOR), Mao, Xuyan3 (AUTHOR), Sun, Dezhi4 (AUTHOR), Liu, Jun3 (AUTHOR), Zhou, Yu5 (AUTHOR), Xu, Xiangyu3 (AUTHOR) xuxiangyu1212@163.com
Source: IET Nanobiotechnology (Wiley-Blackwell). Jun2020, Vol. 14 Issue 4, p308-313. 6p.
Abstract: The binding reaction of reduced graphene oxide–silver nanocomposites (rGO–AgNCs) with calf thymus single‐stranded DNA (ssDNA) was studied by ultraviolet–visible absorption, fluorescence spectroscopy and circular dichroism (CD), using berberine hemisulphate (BR) dye as a fluorescence probe. The absorbance of ssDNA increases, but the fluorescence intensity is quenched with the addition of rGO–AgNCs. The binding of rGO–AgNCs with ssDNA was able to increase the quenching effects of BR and ssDNA, and induce the changes in CD spectra. All of the evidence indicated that there was a relatively strong interaction between ssDNA and rGO–AgNCs. The data obtained from fluorescence experiments revealed that the quenching process of ssDNA caused by rGO–AgNCs is primarily due to complex formation, i.e. static quenching. The increasing trend of the binding equilibrium constant (K a) with rising temperature indicated that the binding process was an endothermic reaction. The calculated thermodynamic parameters showed that the binding process was thermodynamically spontaneous, and hydrophobic association played predominant roles in the binding of ssDNA to the surface of rGO–AgNCs. [ABSTRACT FROM AUTHOR]
Copyright of IET Nanobiotechnology (Wiley-Blackwell) is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 148455792
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  Data: Studies on binding of single‐stranded DNA with reduced graphene oxide–silver nanocomposites.
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  Data: <searchLink fieldCode="AR" term="%22Li%2C+Xi%22">Li, Xi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yang%2C+Linqing%22">Yang, Linqing</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Yunfei%22">Wang, Yunfei</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Du%2C+Zhongyu%22">Du, Zhongyu</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mao%2C+Xuyan%22">Mao, Xuyan</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sun%2C+Dezhi%22">Sun, Dezhi</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Jun%22">Liu, Jun</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhou%2C+Yu%22">Zhou, Yu</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xu%2C+Xiangyu%22">Xu, Xiangyu</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> xuxiangyu1212@163.com</i>
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  Data: <searchLink fieldCode="JN" term="%22IET+Nanobiotechnology+%28Wiley-Blackwell%29%22">IET Nanobiotechnology (Wiley-Blackwell)</searchLink>. Jun2020, Vol. 14 Issue 4, p308-313. 6p.
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The binding reaction of reduced graphene oxide–silver nanocomposites (rGO–AgNCs) with calf thymus single‐stranded DNA (ssDNA) was studied by ultraviolet–visible absorption, fluorescence spectroscopy and circular dichroism (CD), using berberine hemisulphate (BR) dye as a fluorescence probe. The absorbance of ssDNA increases, but the fluorescence intensity is quenched with the addition of rGO–AgNCs. The binding of rGO–AgNCs with ssDNA was able to increase the quenching effects of BR and ssDNA, and induce the changes in CD spectra. All of the evidence indicated that there was a relatively strong interaction between ssDNA and rGO–AgNCs. The data obtained from fluorescence experiments revealed that the quenching process of ssDNA caused by rGO–AgNCs is primarily due to complex formation, i.e. static quenching. The increasing trend of the binding equilibrium constant (K a) with rising temperature indicated that the binding process was an endothermic reaction. The calculated thermodynamic parameters showed that the binding process was thermodynamically spontaneous, and hydrophobic association played predominant roles in the binding of ssDNA to the surface of rGO–AgNCs. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of IET Nanobiotechnology (Wiley-Blackwell) is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1049/iet-nbt.2019.0377
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        Text: English
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              M: 06
              Text: Jun2020
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              Y: 2020
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