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Fast and cost-effective screening for SARS-CoV-2 variants in a routine diagnostic setting.

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Title: Fast and cost-effective screening for SARS-CoV-2 variants in a routine diagnostic setting.
Authors: Durner, Jürgen1,2 (AUTHOR) juergen.durner@med.uni-muenchen.de, Burggraf, Siegfried1 (AUTHOR), Czibere, Ludwig1 (AUTHOR), Tehrani, Arman1 (AUTHOR), Watts, David C.3 (AUTHOR), Becker, Marc1,2 (AUTHOR)
Source: Dental Materials. Mar2021, Vol. 37 Issue 3, pe95-e97. 3p.
Subjects: SARS-CoV-2, Clinical indications, Medical personnel
Geographic Terms: United Kingdom, South Africa
Abstract: The aim is to recommend a fast and cost-effective screening procedure for UK/SA SARS-CoV-2 variants in a routing diagnostic setting. A rapid procedure using qPCR is described to provide clinicians with information about the two currently most prevalent variants (B1.1.7 and B1.351) that harbour receptor binding domain mutation N501Y. The N501Y specific assay only delivers an amplification signal if the Y501 variant is present. 436 samples initially screened positive for SARS-CoV-2 were randomly selected. Only one of these samples showed a fluorescence signal increase indicative for the Y501 variant. The remaining 435 samples had a melting peak at 54 °C indicating the N501 wildtype. The screening of a broad population base can still be performed with the established test system. In case of a positive test for SARS-CoV-2 and corresponding clinical and anamnestic indications, a second qPCR for the mutation N501Y can follow and deliver the result to public health authorities and to the treating physician within a few hours. [ABSTRACT FROM AUTHOR]
Copyright of Dental Materials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
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PubType: Academic Journal
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  Data: Fast and cost-effective screening for SARS-CoV-2 variants in a routine diagnostic setting.
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  Data: <searchLink fieldCode="JN" term="%22Dental+Materials%22">Dental Materials</searchLink>. Mar2021, Vol. 37 Issue 3, pe95-e97. 3p.
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  Data: <searchLink fieldCode="DE" term="%22SARS-CoV-2%22">SARS-CoV-2</searchLink><br /><searchLink fieldCode="DE" term="%22Clinical+indications%22">Clinical indications</searchLink><br /><searchLink fieldCode="DE" term="%22Medical+personnel%22">Medical personnel</searchLink>
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  Label: Abstract
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  Data: The aim is to recommend a fast and cost-effective screening procedure for UK/SA SARS-CoV-2 variants in a routing diagnostic setting. A rapid procedure using qPCR is described to provide clinicians with information about the two currently most prevalent variants (B1.1.7 and B1.351) that harbour receptor binding domain mutation N501Y. The N501Y specific assay only delivers an amplification signal if the Y501 variant is present. 436 samples initially screened positive for SARS-CoV-2 were randomly selected. Only one of these samples showed a fluorescence signal increase indicative for the Y501 variant. The remaining 435 samples had a melting peak at 54 °C indicating the N501 wildtype. The screening of a broad population base can still be performed with the established test system. In case of a positive test for SARS-CoV-2 and corresponding clinical and anamnestic indications, a second qPCR for the mutation N501Y can follow and deliver the result to public health authorities and to the treating physician within a few hours. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Dental Materials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.dental.2021.01.015
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        Text: English
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              M: 03
              Text: Mar2021
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