UCH‐L3 promotes non‐small cell lung cancer proliferation via accelerating cell cycle and inhibiting cell apoptosis.
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| Title: | UCH‐L3 promotes non‐small cell lung cancer proliferation via accelerating cell cycle and inhibiting cell apoptosis. |
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| Authors: | Li, Guoping1 (AUTHOR), Jin, Xiaosheng1 (AUTHOR), Zheng, Jisheng1 (AUTHOR), Jiang, Na1 (AUTHOR), Shi, Wang2 (AUTHOR) WangShifgh@163.com |
| Source: | Biotechnology & Applied Biochemistry. Feb2021, Vol. 68 Issue 1, p165-172. 8p. |
| Subjects: | Cell cycle, Cancer cell proliferation, Inhibition of cellular proliferation, Cell proliferation, Lung cancer |
| Abstract: | Ubiquitin C‐terminal hydrolase‐L3 (UCH‐L3) is a deubiquitinase that has a crucial role in oncogenesis. This study was aimed to explore the biological function of UCH‐L3 in non‐small cell lung cancer (NSCLC). Bioinformatics analysis was used to detect UCH‐L3 expression in NSCLC tissues and normal lung tissues, and to analyze the relationship between UCH‐L3 expression and survival of patients. qRT‐PCR and western blotting assays were used to detect UCH‐L3 expression in NSCLC tumor tissues and adjacent normal tissues. CCK‐8 assay was performed to examine the effect of UCH‐L3 on NSCLC cell proliferation. Flow cytometry assay was conducted to examine the effect of UCH‐L3 on NSCLC cell cycle and apoptosis. The expression of UCH‐L3 in NSCLC tissues was markedly higher than in normal lung tissues, and high expression of UCH‐L3 was positively associated with the poor survival of patients. UCH‐L3 knockdown significantly inhibited the proliferation of NSCLC cells, whereas UCH‐L3 overexpression had the opposite effect. Moreover, UCH‐L3 promoted NSCLC cells proliferation via accelerating cell cycle and inhibiting cell apoptosis. UCH‐L3 is upregulated in NSCLC and positively associated with the poor survival, and its expression contributes to NSCLC cell proliferation by accelerating cell cycle and inhibiting cell apoptosis. [ABSTRACT FROM AUTHOR] |
| Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 149148159 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: UCH‐L3 promotes non‐small cell lung cancer proliferation via accelerating cell cycle and inhibiting cell apoptosis. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Li%2C+Guoping%22">Li, Guoping</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jin%2C+Xiaosheng%22">Jin, Xiaosheng</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zheng%2C+Jisheng%22">Zheng, Jisheng</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jiang%2C+Na%22">Jiang, Na</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Shi%2C+Wang%22">Shi, Wang</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> WangShifgh@163.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Biotechnology+%26+Applied+Biochemistry%22">Biotechnology & Applied Biochemistry</searchLink>. Feb2021, Vol. 68 Issue 1, p165-172. 8p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Cell+cycle%22">Cell cycle</searchLink><br /><searchLink fieldCode="DE" term="%22Cancer+cell+proliferation%22">Cancer cell proliferation</searchLink><br /><searchLink fieldCode="DE" term="%22Inhibition+of+cellular+proliferation%22">Inhibition of cellular proliferation</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+proliferation%22">Cell proliferation</searchLink><br /><searchLink fieldCode="DE" term="%22Lung+cancer%22">Lung cancer</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Ubiquitin C‐terminal hydrolase‐L3 (UCH‐L3) is a deubiquitinase that has a crucial role in oncogenesis. This study was aimed to explore the biological function of UCH‐L3 in non‐small cell lung cancer (NSCLC). Bioinformatics analysis was used to detect UCH‐L3 expression in NSCLC tissues and normal lung tissues, and to analyze the relationship between UCH‐L3 expression and survival of patients. qRT‐PCR and western blotting assays were used to detect UCH‐L3 expression in NSCLC tumor tissues and adjacent normal tissues. CCK‐8 assay was performed to examine the effect of UCH‐L3 on NSCLC cell proliferation. Flow cytometry assay was conducted to examine the effect of UCH‐L3 on NSCLC cell cycle and apoptosis. The expression of UCH‐L3 in NSCLC tissues was markedly higher than in normal lung tissues, and high expression of UCH‐L3 was positively associated with the poor survival of patients. UCH‐L3 knockdown significantly inhibited the proliferation of NSCLC cells, whereas UCH‐L3 overexpression had the opposite effect. Moreover, UCH‐L3 promoted NSCLC cells proliferation via accelerating cell cycle and inhibiting cell apoptosis. UCH‐L3 is upregulated in NSCLC and positively associated with the poor survival, and its expression contributes to NSCLC cell proliferation by accelerating cell cycle and inhibiting cell apoptosis. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/bab.1909 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 165 Subjects: – SubjectFull: Cell cycle Type: general – SubjectFull: Cancer cell proliferation Type: general – SubjectFull: Inhibition of cellular proliferation Type: general – SubjectFull: Cell proliferation Type: general – SubjectFull: Lung cancer Type: general Titles: – TitleFull: UCH‐L3 promotes non‐small cell lung cancer proliferation via accelerating cell cycle and inhibiting cell apoptosis. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Li, Guoping – PersonEntity: Name: NameFull: Jin, Xiaosheng – PersonEntity: Name: NameFull: Zheng, Jisheng – PersonEntity: Name: NameFull: Jiang, Na – PersonEntity: Name: NameFull: Shi, Wang IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 02 Text: Feb2021 Type: published Y: 2021 Identifiers: – Type: issn-print Value: 08854513 Numbering: – Type: volume Value: 68 – Type: issue Value: 1 Titles: – TitleFull: Biotechnology & Applied Biochemistry Type: main |
| ResultId | 1 |
