Molecular weight and guluronic/mannuronic ratio of alginate produced by Azotobacter vinelandii at two bioreactor scales under diazotrophic conditions.
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| Title: | Molecular weight and guluronic/mannuronic ratio of alginate produced by Azotobacter vinelandii at two bioreactor scales under diazotrophic conditions. |
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| Authors: | Díaz-Barrera, Alvaro1 (AUTHOR) alvaro.diaz@pucv.cl, Sanchez-Rosales, Francisco1,2 (AUTHOR), Padilla-Córdova, Claudio1 (AUTHOR), Andler, Rodrigo3 (AUTHOR), Peña, Carlos4 (AUTHOR) |
| Source: | Bioprocess & Biosystems Engineering. Jun2021, Vol. 44 Issue 6, p1275-1287. 13p. |
| Subjects: | Molecular weights, Alginic acid, Alginates, Azotobacter, Cell respiration, Oxygen consumption |
| Abstract: | Alginates can be used to elaborate hydrogels, and their properties depend on the molecular weight (MW) and the guluronic (G) and mannuronic (M) composition. In this study, the MW and G/M ratio were evaluated in cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs) under diazotrophic conditions. An increase in the maximum OTR (OTRmax) improved the alginate production, reaching 3.3 ± 0.2 g L−1. In the cultures conducted to an OTR of 10.4 mmol L−1 h−1 (500 rpm), the G/M increased during the cell growth phase and decreased during the stationary phase; whereas, in the cultures at 19.2 mmol L−1 h−1 was constant throughout the cultivation. A higher alginate MW (520 ± 43 kDa) and G/M ratio (0.86 ± 0.01) were obtained in the cultures conducted at 10.4 mmol L−1 h−1. The OTR as a criterion to scale up alginate production allowed to replicate the concentration and the alginate production rate; however, it was not possible reproduce the MW and G/M ratio. Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g−1 h−1) the alginate MW was similar (approximately 365 kDa) in both scales. The evidences revealed that the qO2 can be a parameter adequate to produce alginate MW similar in two bioreactor scales. Overall, the results have shown that the alginate composition could be affected by cellular respiration, and from a technological perspective the evidences contribute to the design process based on oxygen consumption to produce alginates defined. [ABSTRACT FROM AUTHOR] |
| Copyright of Bioprocess & Biosystems Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Items | – Name: Title Label: Title Group: Ti Data: Molecular weight and guluronic/mannuronic ratio of alginate produced by Azotobacter vinelandii at two bioreactor scales under diazotrophic conditions. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Díaz-Barrera%2C+Alvaro%22">Díaz-Barrera, Alvaro</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> alvaro.diaz@pucv.cl</i><br /><searchLink fieldCode="AR" term="%22Sanchez-Rosales%2C+Francisco%22">Sanchez-Rosales, Francisco</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Padilla-Córdova%2C+Claudio%22">Padilla-Córdova, Claudio</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Andler%2C+Rodrigo%22">Andler, Rodrigo</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Peña%2C+Carlos%22">Peña, Carlos</searchLink><relatesTo>4</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Bioprocess+%26+Biosystems+Engineering%22">Bioprocess & Biosystems Engineering</searchLink>. Jun2021, Vol. 44 Issue 6, p1275-1287. 13p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Molecular+weights%22">Molecular weights</searchLink><br /><searchLink fieldCode="DE" term="%22Alginic+acid%22">Alginic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Alginates%22">Alginates</searchLink><br /><searchLink fieldCode="DE" term="%22Azotobacter%22">Azotobacter</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+respiration%22">Cell respiration</searchLink><br /><searchLink fieldCode="DE" term="%22Oxygen+consumption%22">Oxygen consumption</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Alginates can be used to elaborate hydrogels, and their properties depend on the molecular weight (MW) and the guluronic (G) and mannuronic (M) composition. In this study, the MW and G/M ratio were evaluated in cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs) under diazotrophic conditions. An increase in the maximum OTR (OTRmax) improved the alginate production, reaching 3.3 ± 0.2 g L−1. In the cultures conducted to an OTR of 10.4 mmol L−1 h−1 (500 rpm), the G/M increased during the cell growth phase and decreased during the stationary phase; whereas, in the cultures at 19.2 mmol L−1 h−1 was constant throughout the cultivation. A higher alginate MW (520 ± 43 kDa) and G/M ratio (0.86 ± 0.01) were obtained in the cultures conducted at 10.4 mmol L−1 h−1. The OTR as a criterion to scale up alginate production allowed to replicate the concentration and the alginate production rate; however, it was not possible reproduce the MW and G/M ratio. Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g−1 h−1) the alginate MW was similar (approximately 365 kDa) in both scales. The evidences revealed that the qO2 can be a parameter adequate to produce alginate MW similar in two bioreactor scales. Overall, the results have shown that the alginate composition could be affected by cellular respiration, and from a technological perspective the evidences contribute to the design process based on oxygen consumption to produce alginates defined. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Bioprocess & Biosystems Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00449-021-02532-8 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 13 StartPage: 1275 Subjects: – SubjectFull: Molecular weights Type: general – SubjectFull: Alginic acid Type: general – SubjectFull: Alginates Type: general – SubjectFull: Azotobacter Type: general – SubjectFull: Cell respiration Type: general – SubjectFull: Oxygen consumption Type: general Titles: – TitleFull: Molecular weight and guluronic/mannuronic ratio of alginate produced by Azotobacter vinelandii at two bioreactor scales under diazotrophic conditions. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Díaz-Barrera, Alvaro – PersonEntity: Name: NameFull: Sanchez-Rosales, Francisco – PersonEntity: Name: NameFull: Padilla-Córdova, Claudio – PersonEntity: Name: NameFull: Andler, Rodrigo – PersonEntity: Name: NameFull: Peña, Carlos IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: Jun2021 Type: published Y: 2021 Identifiers: – Type: issn-print Value: 16157591 Numbering: – Type: volume Value: 44 – Type: issue Value: 6 Titles: – TitleFull: Bioprocess & Biosystems Engineering Type: main |
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