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Authentication of sea cucumber products using NGS-based DNA mini-barcoding.

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Title: Authentication of sea cucumber products using NGS-based DNA mini-barcoding.
Authors: Xing, Ran-Ran1 (AUTHOR), Hu, Ran-Ran1,2 (AUTHOR), Wang, Nan1 (AUTHOR), Zhang, Jiu-Kai1 (AUTHOR), Ge, Yi-Qiang2,3 (AUTHOR), Chen, Ying1 (AUTHOR) chenyingcaiq@163.com
Source: Food Control. Nov2021, Vol. 129, pN.PAG-N.PAG. 1p.
Subjects: Sea cucumbers, DNA sequencing, Cytochrome oxidase, DNA, Nucleotide sequencing
Geographic Terms: Asia
Abstract: Sea cucumbers are widely consumed in Asia. This study developed a novel DNA mini-barcode mitochondrial cytochrome c oxidase (COI) of 257 bp to discriminate sea cucumbers species in both raw and processed forms. We designed and compared four mini-barcoding primer sets based on PCR amplification rates against 40 authenticated sea cucumber species. The top-performing primer set was successful for most tested sea cucumber varieties. We also tested the DNA mini-barcode COI in 12 highly processed sea cucumber products using both Sanger sequencing and next-generation DNA sequencing (NGS). This is the first report of highly processed sea cucumber products that detected fraudulent replacement of sea cucumber by one or a blend of species using the NGS approach. The established DNA mini-barcoding system revealed that 83.33% (10/12) of highly processed sea cucumber products were mislabeled. This study identified a need for more regulation of the sea cucumber trade and suggested that DNA mini-barcoding combined with NGS could complement existing sea cucumber identification strategies. • Novel COI mini-barcode (257 bp) was developed to identify sea cucumber species. • Combination of mini-barcode with next generation sequencing for authenticating processed sea cucumber products. • DNA mini-barcoding system is effective in authenticating raw and processed sea cucumber products. • Detection of mislabeling in 83.33% of highly processed sea cucumber products. [ABSTRACT FROM AUTHOR]
Copyright of Food Control is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
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Header DbId: egs
DbLabel: Engineering Source
An: 150771413
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Label: Title
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  Data: Authentication of sea cucumber products using NGS-based DNA mini-barcoding.
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  Data: <searchLink fieldCode="AR" term="%22Xing%2C+Ran-Ran%22">Xing, Ran-Ran</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hu%2C+Ran-Ran%22">Hu, Ran-Ran</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Nan%22">Wang, Nan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Jiu-Kai%22">Zhang, Jiu-Kai</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ge%2C+Yi-Qiang%22">Ge, Yi-Qiang</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chen%2C+Ying%22">Chen, Ying</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> chenyingcaiq@163.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Food+Control%22">Food Control</searchLink>. Nov2021, Vol. 129, pN.PAG-N.PAG. 1p.
– Name: Subject
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  Data: <searchLink fieldCode="DE" term="%22Sea+cucumbers%22">Sea cucumbers</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+sequencing%22">DNA sequencing</searchLink><br /><searchLink fieldCode="DE" term="%22Cytochrome+oxidase%22">Cytochrome oxidase</searchLink><br /><searchLink fieldCode="DE" term="%22DNA%22">DNA</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleotide+sequencing%22">Nucleotide sequencing</searchLink>
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  Data: <searchLink fieldCode="DE" term="%22Asia%22">Asia</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Sea cucumbers are widely consumed in Asia. This study developed a novel DNA mini-barcode mitochondrial cytochrome c oxidase (COI) of 257 bp to discriminate sea cucumbers species in both raw and processed forms. We designed and compared four mini-barcoding primer sets based on PCR amplification rates against 40 authenticated sea cucumber species. The top-performing primer set was successful for most tested sea cucumber varieties. We also tested the DNA mini-barcode COI in 12 highly processed sea cucumber products using both Sanger sequencing and next-generation DNA sequencing (NGS). This is the first report of highly processed sea cucumber products that detected fraudulent replacement of sea cucumber by one or a blend of species using the NGS approach. The established DNA mini-barcoding system revealed that 83.33% (10/12) of highly processed sea cucumber products were mislabeled. This study identified a need for more regulation of the sea cucumber trade and suggested that DNA mini-barcoding combined with NGS could complement existing sea cucumber identification strategies. • Novel COI mini-barcode (257 bp) was developed to identify sea cucumber species. • Combination of mini-barcode with next generation sequencing for authenticating processed sea cucumber products. • DNA mini-barcoding system is effective in authenticating raw and processed sea cucumber products. • Detection of mislabeling in 83.33% of highly processed sea cucumber products. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Food Control is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.foodcont.2021.108199
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      – Code: eng
        Text: English
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        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Sea cucumbers
        Type: general
      – SubjectFull: DNA sequencing
        Type: general
      – SubjectFull: Cytochrome oxidase
        Type: general
      – SubjectFull: DNA
        Type: general
      – SubjectFull: Nucleotide sequencing
        Type: general
      – SubjectFull: Asia
        Type: general
    Titles:
      – TitleFull: Authentication of sea cucumber products using NGS-based DNA mini-barcoding.
        Type: main
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            NameFull: Xing, Ran-Ran
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            NameFull: Hu, Ran-Ran
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            NameFull: Wang, Nan
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            NameFull: Zhang, Jiu-Kai
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            NameFull: Ge, Yi-Qiang
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            – D: 01
              M: 11
              Text: Nov2021
              Type: published
              Y: 2021
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              Value: 09567135
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              Value: 129
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            – TitleFull: Food Control
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