Authentication of sea cucumber products using NGS-based DNA mini-barcoding.
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| Title: | Authentication of sea cucumber products using NGS-based DNA mini-barcoding. |
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| Authors: | Xing, Ran-Ran1 (AUTHOR), Hu, Ran-Ran1,2 (AUTHOR), Wang, Nan1 (AUTHOR), Zhang, Jiu-Kai1 (AUTHOR), Ge, Yi-Qiang2,3 (AUTHOR), Chen, Ying1 (AUTHOR) chenyingcaiq@163.com |
| Source: | Food Control. Nov2021, Vol. 129, pN.PAG-N.PAG. 1p. |
| Subjects: | Sea cucumbers, DNA sequencing, Cytochrome oxidase, DNA, Nucleotide sequencing |
| Geographic Terms: | Asia |
| Abstract: | Sea cucumbers are widely consumed in Asia. This study developed a novel DNA mini-barcode mitochondrial cytochrome c oxidase (COI) of 257 bp to discriminate sea cucumbers species in both raw and processed forms. We designed and compared four mini-barcoding primer sets based on PCR amplification rates against 40 authenticated sea cucumber species. The top-performing primer set was successful for most tested sea cucumber varieties. We also tested the DNA mini-barcode COI in 12 highly processed sea cucumber products using both Sanger sequencing and next-generation DNA sequencing (NGS). This is the first report of highly processed sea cucumber products that detected fraudulent replacement of sea cucumber by one or a blend of species using the NGS approach. The established DNA mini-barcoding system revealed that 83.33% (10/12) of highly processed sea cucumber products were mislabeled. This study identified a need for more regulation of the sea cucumber trade and suggested that DNA mini-barcoding combined with NGS could complement existing sea cucumber identification strategies. • Novel COI mini-barcode (257 bp) was developed to identify sea cucumber species. • Combination of mini-barcode with next generation sequencing for authenticating processed sea cucumber products. • DNA mini-barcoding system is effective in authenticating raw and processed sea cucumber products. • Detection of mislabeling in 83.33% of highly processed sea cucumber products. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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