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The BOP‐type co‐transcriptional regulator NODULE ROOT1 promotes stem secondary growth of the tropical Cannabaceae tree Parasponia andersonii.

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Title: The BOP‐type co‐transcriptional regulator NODULE ROOT1 promotes stem secondary growth of the tropical Cannabaceae tree Parasponia andersonii.
Authors: Shen, Defeng1 (AUTHOR), Holmer, Rens1 (AUTHOR), Kulikova, Olga1 (AUTHOR), Mannapperuma, Chanaka2 (AUTHOR), Street, Nathaniel R.2 (AUTHOR), Yan, Zhichun1 (AUTHOR), Maden, Thomas1 (AUTHOR), Bu, Fengjiao1 (AUTHOR), Zhang, Yuanyuan3 (AUTHOR), Geurts, Rene1 (AUTHOR) rene.geurts@wur.nl, Magne, Kévin1 (AUTHOR) kevin_magne@hotmail.fr
Source: Plant Journal. Jun2021, Vol. 106 Issue 5, p1366-1386. 21p.
Subjects: Cannabaceae, Reverse genetics, Genetic regulation, Root-tubercles, Cambium, Legumes, Secondary metabolism
Abstract: Summary: Tree stems undergo a massive secondary growth in which secondary xylem and phloem tissues arise from the vascular cambium. Vascular cambium activity is driven by endogenous developmental signalling cues and environmental stimuli. Current knowledge regarding the genetic regulation of cambium activity and secondary growth is still far from complete. The tropical Cannabaceae tree Parasponia andersonii is a non‐legume research model of nitrogen‐fixing root nodulation. Parasponia andersonii can be transformed efficiently, making it amenable for CRISPR‐Cas9‐mediated reverse genetics. We considered whether P. andersonii also could be used as a complementary research system to investigate tree‐related traits, including secondary growth. We established a developmental map of stem secondary growth in P. andersonii plantlets. Subsequently, we showed that the expression of the co‐transcriptional regulator PanNODULE ROOT1 (PanNOOT1) is essential for controlling this process. PanNOOT1 is orthologous to Arabidopsis thaliana BLADE‐ON‐PETIOLE1 (AtBOP1) and AtBOP2, which are involved in the meristem‐to‐organ‐boundary maintenance. Moreover, in species forming nitrogen‐fixing root nodules, NOOT1 is known to function as a key nodule identity gene. Parasponia andersonii CRISPR‐Cas9 loss‐of‐function Pannoot1 mutants are altered in the development of the xylem and phloem tissues without apparent disturbance of the cambium organization and size. Transcriptomic analysis showed that the expression of key secondary growth‐related genes is significantly down‐regulated in Pannoot1 mutants. This allows us to conclude that PanNOOT1 positively contributes to the regulation of stem secondary growth. Our work also demonstrates that P. andersonii can serve as a tree research system. Significance Statement: Parasponia andersonii Pannoot1 CRISPR‐Cas9 knockout mutants are altered for secondary growth development. We show a new role for NBCL genes in tree stem secondary growth and provide a proof of concept that P. andersonii is suited for the investigation of tree‐related traits. [ABSTRACT FROM AUTHOR]
Copyright of Plant Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
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AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: The BOP‐type co‐transcriptional regulator NODULE ROOT1 promotes stem secondary growth of the tropical Cannabaceae tree Parasponia andersonii.
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  Data: <searchLink fieldCode="AR" term="%22Shen%2C+Defeng%22">Shen, Defeng</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Holmer%2C+Rens%22">Holmer, Rens</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kulikova%2C+Olga%22">Kulikova, Olga</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mannapperuma%2C+Chanaka%22">Mannapperuma, Chanaka</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Street%2C+Nathaniel+R%2E%22">Street, Nathaniel R.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yan%2C+Zhichun%22">Yan, Zhichun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Maden%2C+Thomas%22">Maden, Thomas</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bu%2C+Fengjiao%22">Bu, Fengjiao</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Yuanyuan%22">Zhang, Yuanyuan</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Geurts%2C+Rene%22">Geurts, Rene</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> rene.geurts@wur.nl</i><br /><searchLink fieldCode="AR" term="%22Magne%2C+Kévin%22">Magne, Kévin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> kevin_magne@hotmail.fr</i>
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  Data: <searchLink fieldCode="JN" term="%22Plant+Journal%22">Plant Journal</searchLink>. Jun2021, Vol. 106 Issue 5, p1366-1386. 21p.
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  Data: <searchLink fieldCode="DE" term="%22Cannabaceae%22">Cannabaceae</searchLink><br /><searchLink fieldCode="DE" term="%22Reverse+genetics%22">Reverse genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+regulation%22">Genetic regulation</searchLink><br /><searchLink fieldCode="DE" term="%22Root-tubercles%22">Root-tubercles</searchLink><br /><searchLink fieldCode="DE" term="%22Cambium%22">Cambium</searchLink><br /><searchLink fieldCode="DE" term="%22Legumes%22">Legumes</searchLink><br /><searchLink fieldCode="DE" term="%22Secondary+metabolism%22">Secondary metabolism</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Summary: Tree stems undergo a massive secondary growth in which secondary xylem and phloem tissues arise from the vascular cambium. Vascular cambium activity is driven by endogenous developmental signalling cues and environmental stimuli. Current knowledge regarding the genetic regulation of cambium activity and secondary growth is still far from complete. The tropical Cannabaceae tree Parasponia andersonii is a non‐legume research model of nitrogen‐fixing root nodulation. Parasponia andersonii can be transformed efficiently, making it amenable for CRISPR‐Cas9‐mediated reverse genetics. We considered whether P. andersonii also could be used as a complementary research system to investigate tree‐related traits, including secondary growth. We established a developmental map of stem secondary growth in P. andersonii plantlets. Subsequently, we showed that the expression of the co‐transcriptional regulator PanNODULE ROOT1 (PanNOOT1) is essential for controlling this process. PanNOOT1 is orthologous to Arabidopsis thaliana BLADE‐ON‐PETIOLE1 (AtBOP1) and AtBOP2, which are involved in the meristem‐to‐organ‐boundary maintenance. Moreover, in species forming nitrogen‐fixing root nodules, NOOT1 is known to function as a key nodule identity gene. Parasponia andersonii CRISPR‐Cas9 loss‐of‐function Pannoot1 mutants are altered in the development of the xylem and phloem tissues without apparent disturbance of the cambium organization and size. Transcriptomic analysis showed that the expression of key secondary growth‐related genes is significantly down‐regulated in Pannoot1 mutants. This allows us to conclude that PanNOOT1 positively contributes to the regulation of stem secondary growth. Our work also demonstrates that P. andersonii can serve as a tree research system. Significance Statement: Parasponia andersonii Pannoot1 CRISPR‐Cas9 knockout mutants are altered for secondary growth development. We show a new role for NBCL genes in tree stem secondary growth and provide a proof of concept that P. andersonii is suited for the investigation of tree‐related traits. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Plant Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1111/tpj.15242
    Languages:
      – Code: eng
        Text: English
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      Pagination:
        PageCount: 21
        StartPage: 1366
    Subjects:
      – SubjectFull: Cannabaceae
        Type: general
      – SubjectFull: Reverse genetics
        Type: general
      – SubjectFull: Genetic regulation
        Type: general
      – SubjectFull: Root-tubercles
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      – SubjectFull: Cambium
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      – SubjectFull: Legumes
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      – SubjectFull: Secondary metabolism
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      – TitleFull: The BOP‐type co‐transcriptional regulator NODULE ROOT1 promotes stem secondary growth of the tropical Cannabaceae tree Parasponia andersonii.
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              Text: Jun2021
              Type: published
              Y: 2021
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