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Candidate Rlm6 resistance genes against Leptosphaeria. maculans identified through a genome-wide association study in Brassica juncea (L.) Czern.

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Title: Candidate Rlm6 resistance genes against Leptosphaeria. maculans identified through a genome-wide association study in Brassica juncea (L.) Czern.
Authors: Yang, Hua1,2 (AUTHOR), Mohd Saad, Nur Shuhadah2 (AUTHOR), Ibrahim, Muhammad Ishaq3 (AUTHOR), Bayer, Philipp E.2 (AUTHOR), Neik, Ting Xiang2 (AUTHOR), Severn-Ellis, Anita A.2 (AUTHOR), Pradhan, Aneeta2 (AUTHOR), Tirnaz, Soodeh2 (AUTHOR), Edwards, David2 (AUTHOR), Batley, Jacqueline2 (AUTHOR) jacqueline.batley@uwa.edu.au
Source: Theoretical & Applied Genetics. Jul2021, Vol. 134 Issue 7, p2035-2050. 16p.
Subjects: Brassica juncea, Genome-wide association studies, Leptosphaeria maculans, Single nucleotide polymorphisms, Drought tolerance, Cotyledons, Germplasm, Genes
Abstract: Key message: One hundred and sixty-seven B. juncea varieties were genotyped on the 90K Brassica assay (42,914 SNPs), which led to the identification of sixteen candidate genes for Rlm6. Brassica species are at high risk of severe crop loss due to pathogens, especially Leptosphaeria maculans (the causal agent of blackleg). Brassica juncea (L.) Czern is an important germplasm resource for canola improvement, due to its good agronomic traits, such as heat and drought tolerance and high blackleg resistance. The present study is the first using genome-wide association studies to identify candidate genes for blackleg resistance in B. juncea based on genome-wide SNPs obtained from the Illumina Infinium 90 K Brassica SNP array. The verification of Rlm6 in B. juncea was performed through a cotyledon infection test. Genotyping 42,914 single nucleotide polymorphisms (SNPs) in a panel of 167 B. juncea lines revealed a total of seven SNPs significantly associated with Rlm6 on chromosomes A07 and B04 in B. juncea. Furthermore, 16 candidate Rlm6 genes were found in these regions, defined as nucleotide binding site leucine-rich-repeat (NLR), leucine-rich repeat RLK (LRR-RLK) and LRR-RLP genes. This study will give insights into the blackleg resistance in B. juncea and facilitate identification of functional blackleg resistance genes which can be used in Brassica breeding. [ABSTRACT FROM AUTHOR]
Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Candidate Rlm6 resistance genes against Leptosphaeria. maculans identified through a genome-wide association study in Brassica juncea (L.) Czern.
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  Data: <searchLink fieldCode="AR" term="%22Yang%2C+Hua%22">Yang, Hua</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mohd+Saad%2C+Nur+Shuhadah%22">Mohd Saad, Nur Shuhadah</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ibrahim%2C+Muhammad+Ishaq%22">Ibrahim, Muhammad Ishaq</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bayer%2C+Philipp+E%2E%22">Bayer, Philipp E.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Neik%2C+Ting+Xiang%22">Neik, Ting Xiang</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Severn-Ellis%2C+Anita+A%2E%22">Severn-Ellis, Anita A.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pradhan%2C+Aneeta%22">Pradhan, Aneeta</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tirnaz%2C+Soodeh%22">Tirnaz, Soodeh</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Edwards%2C+David%22">Edwards, David</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Batley%2C+Jacqueline%22">Batley, Jacqueline</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> jacqueline.batley@uwa.edu.au</i>
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  Data: <searchLink fieldCode="DE" term="%22Brassica+juncea%22">Brassica juncea</searchLink><br /><searchLink fieldCode="DE" term="%22Genome-wide+association+studies%22">Genome-wide association studies</searchLink><br /><searchLink fieldCode="DE" term="%22Leptosphaeria+maculans%22">Leptosphaeria maculans</searchLink><br /><searchLink fieldCode="DE" term="%22Single+nucleotide+polymorphisms%22">Single nucleotide polymorphisms</searchLink><br /><searchLink fieldCode="DE" term="%22Drought+tolerance%22">Drought tolerance</searchLink><br /><searchLink fieldCode="DE" term="%22Cotyledons%22">Cotyledons</searchLink><br /><searchLink fieldCode="DE" term="%22Germplasm%22">Germplasm</searchLink><br /><searchLink fieldCode="DE" term="%22Genes%22">Genes</searchLink>
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  Label: Abstract
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  Data: Key message: One hundred and sixty-seven B. juncea varieties were genotyped on the 90K Brassica assay (42,914 SNPs), which led to the identification of sixteen candidate genes for Rlm6. Brassica species are at high risk of severe crop loss due to pathogens, especially Leptosphaeria maculans (the causal agent of blackleg). Brassica juncea (L.) Czern is an important germplasm resource for canola improvement, due to its good agronomic traits, such as heat and drought tolerance and high blackleg resistance. The present study is the first using genome-wide association studies to identify candidate genes for blackleg resistance in B. juncea based on genome-wide SNPs obtained from the Illumina Infinium 90 K Brassica SNP array. The verification of Rlm6 in B. juncea was performed through a cotyledon infection test. Genotyping 42,914 single nucleotide polymorphisms (SNPs) in a panel of 167 B. juncea lines revealed a total of seven SNPs significantly associated with Rlm6 on chromosomes A07 and B04 in B. juncea. Furthermore, 16 candidate Rlm6 genes were found in these regions, defined as nucleotide binding site leucine-rich-repeat (NLR), leucine-rich repeat RLK (LRR-RLK) and LRR-RLP genes. This study will give insights into the blackleg resistance in B. juncea and facilitate identification of functional blackleg resistance genes which can be used in Brassica breeding. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1007/s00122-021-03803-4
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        Text: English
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        PageCount: 16
        StartPage: 2035
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      – SubjectFull: Brassica juncea
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      – SubjectFull: Genome-wide association studies
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