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Insensitivity of dental pulp stem cells migration to substrate stiffness.

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Title: Insensitivity of dental pulp stem cells migration to substrate stiffness.
Authors: Ehlinger, Claire1,2,3 (AUTHOR), Mathieu, Eric1,2,3 (AUTHOR), Rabineau, Morgane1,2,3 (AUTHOR), Ball, Vincent1,2,3 (AUTHOR), Lavalle, Philippe1,2,3 (AUTHOR), Haikel, Youssef1,2,3 (AUTHOR), Vautier, Dominique1,2,3 (AUTHOR) vautier@unistra.fr, Kocgozlu, Leyla1,2,3 (AUTHOR) leyla.kocgozlu@gmail.com
Source: Biomaterials. Aug2021, Vol. 275, pN.PAG-N.PAG. 1p.
Subjects: Dental pulp, Cell migration, Stem cells, Focal adhesions, Extracellular matrix
Abstract: Dental pulp stem cells (DPSCs) are a promising cell source for regeneration of dental pulp. Migration is a key event but influence of the microenvironment rigidity (5 kPa at the center of dental pulp to 20 GPa for the dentin) is largely unknown. Mechanical signals are transmitted from the extracellular matrix to the cytoskeleton, to the nuclei, and to the chromatin, potentially regulating gene expression. To identify the microenvironmental influence on migration, we analyzed motility on PDMS substrates with stiffness increasing from 1.5 kPa up to 2.5 MPa. We found that migration speed slightly increases as substrate stiffness decreases in correlation with decreasing focal adhesion size. Motility is relatively insensitive to substrate stiffness, even on a bi-rigidity PDMS substrate where DPSCs migrate without preferential direction. Migration is independent of both myosin II activity and YAP translocation after myosin II inhibition. Additionally, inhibition of Arp2/3 complex leads to significant speed decrease for all rigidities, suggesting contribution of the lamellipodia in the migration. Interestingly, the chromatin architecture remains stable after a 7-days exposure on the PDMS substrates for all rigidity. To design scaffold mimicking dental pulp environment, similar DPSCs migration for all rigidity, leaves field open to choose this mechanical parameter. [ABSTRACT FROM AUTHOR]
Copyright of Biomaterials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 151608277
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Label: Title
  Group: Ti
  Data: Insensitivity of dental pulp stem cells migration to substrate stiffness.
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  Data: <searchLink fieldCode="AR" term="%22Ehlinger%2C+Claire%22">Ehlinger, Claire</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mathieu%2C+Eric%22">Mathieu, Eric</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Rabineau%2C+Morgane%22">Rabineau, Morgane</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ball%2C+Vincent%22">Ball, Vincent</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lavalle%2C+Philippe%22">Lavalle, Philippe</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Haikel%2C+Youssef%22">Haikel, Youssef</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Vautier%2C+Dominique%22">Vautier, Dominique</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<i> vautier@unistra.fr</i><br /><searchLink fieldCode="AR" term="%22Kocgozlu%2C+Leyla%22">Kocgozlu, Leyla</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<i> leyla.kocgozlu@gmail.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Biomaterials%22">Biomaterials</searchLink>. Aug2021, Vol. 275, pN.PAG-N.PAG. 1p.
– Name: Subject
  Label: Subjects
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  Data: <searchLink fieldCode="DE" term="%22Dental+pulp%22">Dental pulp</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+migration%22">Cell migration</searchLink><br /><searchLink fieldCode="DE" term="%22Stem+cells%22">Stem cells</searchLink><br /><searchLink fieldCode="DE" term="%22Focal+adhesions%22">Focal adhesions</searchLink><br /><searchLink fieldCode="DE" term="%22Extracellular+matrix%22">Extracellular matrix</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Dental pulp stem cells (DPSCs) are a promising cell source for regeneration of dental pulp. Migration is a key event but influence of the microenvironment rigidity (5 kPa at the center of dental pulp to 20 GPa for the dentin) is largely unknown. Mechanical signals are transmitted from the extracellular matrix to the cytoskeleton, to the nuclei, and to the chromatin, potentially regulating gene expression. To identify the microenvironmental influence on migration, we analyzed motility on PDMS substrates with stiffness increasing from 1.5 kPa up to 2.5 MPa. We found that migration speed slightly increases as substrate stiffness decreases in correlation with decreasing focal adhesion size. Motility is relatively insensitive to substrate stiffness, even on a bi-rigidity PDMS substrate where DPSCs migrate without preferential direction. Migration is independent of both myosin II activity and YAP translocation after myosin II inhibition. Additionally, inhibition of Arp2/3 complex leads to significant speed decrease for all rigidities, suggesting contribution of the lamellipodia in the migration. Interestingly, the chromatin architecture remains stable after a 7-days exposure on the PDMS substrates for all rigidity. To design scaffold mimicking dental pulp environment, similar DPSCs migration for all rigidity, leaves field open to choose this mechanical parameter. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Biomaterials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.biomaterials.2021.120969
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      – Code: eng
        Text: English
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        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Dental pulp
        Type: general
      – SubjectFull: Cell migration
        Type: general
      – SubjectFull: Stem cells
        Type: general
      – SubjectFull: Focal adhesions
        Type: general
      – SubjectFull: Extracellular matrix
        Type: general
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      – TitleFull: Insensitivity of dental pulp stem cells migration to substrate stiffness.
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            NameFull: Ehlinger, Claire
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            NameFull: Mathieu, Eric
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            NameFull: Rabineau, Morgane
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            NameFull: Ball, Vincent
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            NameFull: Lavalle, Philippe
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            NameFull: Haikel, Youssef
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            – D: 01
              M: 08
              Text: Aug2021
              Type: published
              Y: 2021
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              Value: 275
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            – TitleFull: Biomaterials
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