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Synthesis of sub-50 nm bio-inspired silica particles using a C-terminal-modified ferritin template with a silica-forming peptide.

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Title: Synthesis of sub-50 nm bio-inspired silica particles using a C-terminal-modified ferritin template with a silica-forming peptide.
Authors: Nguyen, Thi Khoa My1,2 (AUTHOR), Ki, Mi Ran1 (AUTHOR), Son, Ryeo Gang1 (AUTHOR), Kim, Kyung Hee1 (AUTHOR), Hong, Junghwa2,3 (AUTHOR), Pack, Seung Pil1 (AUTHOR) spack@korea.ac.kr
Source: Journal of Industrial & Engineering Chemistry. Sep2021, Vol. 101, p262-269. 8p.
Subjects: Cytoskeletal proteins, Ferritin, Silica, Moieties (Chemistry), Doxorubicin, Cancer cells, Peptides, Silica nanoparticles
Abstract: [Display omitted] • Silica forming peptide (SFP) such as Kps or Kpt was fused to C-teminus of ferritin (Fn-cSFP). • Intact Fn-cKps and Fn-cKpt were used as templates for synthesis of biosilica nanoparticles (Fn-cSFP@SiO 2). • The generated Fn-cKps@SiO 2 and Fn-cKpt@SiO 2 were 26 and 28 nm in mean diameter, respectively. • Fn-cSFP@SiO 2 showed a high loading efficiency of doxorubicin (Dox) and pH-responsive release patterns of Dox. Silica-forming peptides (SFPs) and the SFP-modified structural proteins can be used as templates for the synthesis of biosilica nanoparticles (NPs). However, such biomolecule-mediated synthesis showed limitations for the generation of NPs with sizes sub-50 nm. In this study, the SFP sequences (KPSHHHHHTGAN and KPTHHHHHHDG for Kps and Kpt, respectively) were fused to the C-terminus of the human ferritin heavy chain (Fn), resulting in the SFP moieties in the inner space of Fn (termed Fn-cKps and Fn-cKpt). Using Fn-cSFP templates for silicification in two-phase system, the Fn-cKps@SiO 2 and Fn-cKpt@SiO 2 NPs were generated in mean diameters of 26 and 28 nm, respectively. Also, we employed biosilica NPs sub-50 nm for a doxorubicin (Dox) delivery system (application model). Fn-cKpt@SiO 2 NPs exhibited a high loading efficiency compared to Fn-cKpt only (1.7-fold) and prolonged release patterns with Dox. Most importantly, the uptake of Fn-cKpt@SiO 2 into cancer cells was increased so that the efficient delivery of Dox to the cell inside was observed. The uniform generation of biosilica NPs sub-50 nm obtained here is a new achievement. Together with the ideal pH-dependent drug release, the size-controlled design of biosilica will be a useful strategy for efficient delivery of chemical drugs to target cells. [ABSTRACT FROM AUTHOR]
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Abstract:[Display omitted] • Silica forming peptide (SFP) such as Kps or Kpt was fused to C-teminus of ferritin (Fn-cSFP). • Intact Fn-cKps and Fn-cKpt were used as templates for synthesis of biosilica nanoparticles (Fn-cSFP@SiO 2). • The generated Fn-cKps@SiO 2 and Fn-cKpt@SiO 2 were 26 and 28 nm in mean diameter, respectively. • Fn-cSFP@SiO 2 showed a high loading efficiency of doxorubicin (Dox) and pH-responsive release patterns of Dox. Silica-forming peptides (SFPs) and the SFP-modified structural proteins can be used as templates for the synthesis of biosilica nanoparticles (NPs). However, such biomolecule-mediated synthesis showed limitations for the generation of NPs with sizes sub-50 nm. In this study, the SFP sequences (KPSHHHHHTGAN and KPTHHHHHHDG for Kps and Kpt, respectively) were fused to the C-terminus of the human ferritin heavy chain (Fn), resulting in the SFP moieties in the inner space of Fn (termed Fn-cKps and Fn-cKpt). Using Fn-cSFP templates for silicification in two-phase system, the Fn-cKps@SiO 2 and Fn-cKpt@SiO 2 NPs were generated in mean diameters of 26 and 28 nm, respectively. Also, we employed biosilica NPs sub-50 nm for a doxorubicin (Dox) delivery system (application model). Fn-cKpt@SiO 2 NPs exhibited a high loading efficiency compared to Fn-cKpt only (1.7-fold) and prolonged release patterns with Dox. Most importantly, the uptake of Fn-cKpt@SiO 2 into cancer cells was increased so that the efficient delivery of Dox to the cell inside was observed. The uniform generation of biosilica NPs sub-50 nm obtained here is a new achievement. Together with the ideal pH-dependent drug release, the size-controlled design of biosilica will be a useful strategy for efficient delivery of chemical drugs to target cells. [ABSTRACT FROM AUTHOR]
ISSN:1226086X
DOI:10.1016/j.jiec.2021.06.005