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Synthesis of sub-50 nm bio-inspired silica particles using a C-terminal-modified ferritin template with a silica-forming peptide.

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Title: Synthesis of sub-50 nm bio-inspired silica particles using a C-terminal-modified ferritin template with a silica-forming peptide.
Authors: Nguyen, Thi Khoa My1,2 (AUTHOR), Ki, Mi Ran1 (AUTHOR), Son, Ryeo Gang1 (AUTHOR), Kim, Kyung Hee1 (AUTHOR), Hong, Junghwa2,3 (AUTHOR), Pack, Seung Pil1 (AUTHOR) spack@korea.ac.kr
Source: Journal of Industrial & Engineering Chemistry. Sep2021, Vol. 101, p262-269. 8p.
Subjects: Cytoskeletal proteins, Ferritin, Silica, Moieties (Chemistry), Doxorubicin, Cancer cells, Peptides, Silica nanoparticles
Abstract: [Display omitted] • Silica forming peptide (SFP) such as Kps or Kpt was fused to C-teminus of ferritin (Fn-cSFP). • Intact Fn-cKps and Fn-cKpt were used as templates for synthesis of biosilica nanoparticles (Fn-cSFP@SiO 2). • The generated Fn-cKps@SiO 2 and Fn-cKpt@SiO 2 were 26 and 28 nm in mean diameter, respectively. • Fn-cSFP@SiO 2 showed a high loading efficiency of doxorubicin (Dox) and pH-responsive release patterns of Dox. Silica-forming peptides (SFPs) and the SFP-modified structural proteins can be used as templates for the synthesis of biosilica nanoparticles (NPs). However, such biomolecule-mediated synthesis showed limitations for the generation of NPs with sizes sub-50 nm. In this study, the SFP sequences (KPSHHHHHTGAN and KPTHHHHHHDG for Kps and Kpt, respectively) were fused to the C-terminus of the human ferritin heavy chain (Fn), resulting in the SFP moieties in the inner space of Fn (termed Fn-cKps and Fn-cKpt). Using Fn-cSFP templates for silicification in two-phase system, the Fn-cKps@SiO 2 and Fn-cKpt@SiO 2 NPs were generated in mean diameters of 26 and 28 nm, respectively. Also, we employed biosilica NPs sub-50 nm for a doxorubicin (Dox) delivery system (application model). Fn-cKpt@SiO 2 NPs exhibited a high loading efficiency compared to Fn-cKpt only (1.7-fold) and prolonged release patterns with Dox. Most importantly, the uptake of Fn-cKpt@SiO 2 into cancer cells was increased so that the efficient delivery of Dox to the cell inside was observed. The uniform generation of biosilica NPs sub-50 nm obtained here is a new achievement. Together with the ideal pH-dependent drug release, the size-controlled design of biosilica will be a useful strategy for efficient delivery of chemical drugs to target cells. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Industrial & Engineering Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 151631425
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  Data: Synthesis of sub-50 nm bio-inspired silica particles using a C-terminal-modified ferritin template with a silica-forming peptide.
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  Data: <searchLink fieldCode="AR" term="%22Nguyen%2C+Thi+Khoa+My%22">Nguyen, Thi Khoa My</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ki%2C+Mi+Ran%22">Ki, Mi Ran</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Son%2C+Ryeo+Gang%22">Son, Ryeo Gang</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kim%2C+Kyung+Hee%22">Kim, Kyung Hee</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hong%2C+Junghwa%22">Hong, Junghwa</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pack%2C+Seung+Pil%22">Pack, Seung Pil</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> spack@korea.ac.kr</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Industrial+%26+Engineering+Chemistry%22">Journal of Industrial & Engineering Chemistry</searchLink>. Sep2021, Vol. 101, p262-269. 8p.
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  Data: <searchLink fieldCode="DE" term="%22Cytoskeletal+proteins%22">Cytoskeletal proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Ferritin%22">Ferritin</searchLink><br /><searchLink fieldCode="DE" term="%22Silica%22">Silica</searchLink><br /><searchLink fieldCode="DE" term="%22Moieties+%28Chemistry%29%22">Moieties (Chemistry)</searchLink><br /><searchLink fieldCode="DE" term="%22Doxorubicin%22">Doxorubicin</searchLink><br /><searchLink fieldCode="DE" term="%22Cancer+cells%22">Cancer cells</searchLink><br /><searchLink fieldCode="DE" term="%22Peptides%22">Peptides</searchLink><br /><searchLink fieldCode="DE" term="%22Silica+nanoparticles%22">Silica nanoparticles</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: [Display omitted] • Silica forming peptide (SFP) such as Kps or Kpt was fused to C-teminus of ferritin (Fn-cSFP). • Intact Fn-cKps and Fn-cKpt were used as templates for synthesis of biosilica nanoparticles (Fn-cSFP@SiO 2). • The generated Fn-cKps@SiO 2 and Fn-cKpt@SiO 2 were 26 and 28 nm in mean diameter, respectively. • Fn-cSFP@SiO 2 showed a high loading efficiency of doxorubicin (Dox) and pH-responsive release patterns of Dox. Silica-forming peptides (SFPs) and the SFP-modified structural proteins can be used as templates for the synthesis of biosilica nanoparticles (NPs). However, such biomolecule-mediated synthesis showed limitations for the generation of NPs with sizes sub-50 nm. In this study, the SFP sequences (KPSHHHHHTGAN and KPTHHHHHHDG for Kps and Kpt, respectively) were fused to the C-terminus of the human ferritin heavy chain (Fn), resulting in the SFP moieties in the inner space of Fn (termed Fn-cKps and Fn-cKpt). Using Fn-cSFP templates for silicification in two-phase system, the Fn-cKps@SiO 2 and Fn-cKpt@SiO 2 NPs were generated in mean diameters of 26 and 28 nm, respectively. Also, we employed biosilica NPs sub-50 nm for a doxorubicin (Dox) delivery system (application model). Fn-cKpt@SiO 2 NPs exhibited a high loading efficiency compared to Fn-cKpt only (1.7-fold) and prolonged release patterns with Dox. Most importantly, the uptake of Fn-cKpt@SiO 2 into cancer cells was increased so that the efficient delivery of Dox to the cell inside was observed. The uniform generation of biosilica NPs sub-50 nm obtained here is a new achievement. Together with the ideal pH-dependent drug release, the size-controlled design of biosilica will be a useful strategy for efficient delivery of chemical drugs to target cells. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Industrial & Engineering Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.jiec.2021.06.005
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 8
        StartPage: 262
    Subjects:
      – SubjectFull: Cytoskeletal proteins
        Type: general
      – SubjectFull: Ferritin
        Type: general
      – SubjectFull: Silica
        Type: general
      – SubjectFull: Moieties (Chemistry)
        Type: general
      – SubjectFull: Doxorubicin
        Type: general
      – SubjectFull: Cancer cells
        Type: general
      – SubjectFull: Peptides
        Type: general
      – SubjectFull: Silica nanoparticles
        Type: general
    Titles:
      – TitleFull: Synthesis of sub-50 nm bio-inspired silica particles using a C-terminal-modified ferritin template with a silica-forming peptide.
        Type: main
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            NameFull: Nguyen, Thi Khoa My
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            NameFull: Ki, Mi Ran
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            NameFull: Son, Ryeo Gang
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            NameFull: Kim, Kyung Hee
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            NameFull: Hong, Junghwa
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            NameFull: Pack, Seung Pil
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            – D: 25
              M: 09
              Text: Sep2021
              Type: published
              Y: 2021
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              Value: 101
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            – TitleFull: Journal of Industrial & Engineering Chemistry
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