Bone repair assessment of critical size defects in rats treated with mineralized bovine bone (Bio-Oss®) and photobiomodulation therapy: a histomorphometric and immunohistochemical study.
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| Title: | Bone repair assessment of critical size defects in rats treated with mineralized bovine bone (Bio-Oss®) and photobiomodulation therapy: a histomorphometric and immunohistochemical study. |
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| Authors: | Torquato, Letícia Cavassini1 leticia.c.torquato@hotmail.com, Suárez, Eduardo Antonio Chelin1, Bernardo, Daniella Viscensotto1, Pinto, Isis Luzcybel Ribeiro2, Mantovani, Ludmilla Oliveira2, Silva, Thiago Igor Lemes2, Jardini, Maria Aparecida Neves3, Santamaria, Mauro Pedrine3, De Marco, Andrea Carvalho3 |
| Source: | Lasers in Medical Science. Sep2021, Vol. 36 Issue 7, p1515-1525. 11p. |
| Subjects: | Photodynamic therapy, Osteoprotegerin, Immunohistochemistry, Bone regeneration, Immunostaining |
| Abstract: | This study aimed to investigate the effects of administering photobiomodulation therapy (PBM) with bovine bone matrix on critical size defects in rats. Seventy-two adult male rats (albinus, Wistar), 90 days old, were used. Defect of 5 mm in diameter was made in their calvaria. The animals were divided into 4 groups: C-blood clot, B-Bio-Oss®, L-PBM, B+L-Bio-Oss®+PBM. Each group has been subdivided into 07, 30, and 60 days of observation. For PBM, a low GaAlAs energy of 660 nm was irradiated, total energy density of 45 J/cm2. PBM was conducted in a trans-surgical form once only. For immunohistochemistry, a semi-quantitative analysis was made of expression of osteoprotegerin (OPG), nuclear kappa B-factor ligand receptor activator (RANKL), and tartrate-resistant acid phosphatase (TRAP). All histomorphometric data were statistically analyzed by ANOVA and Tukey test, significance level of 5%. The groups that showed the highest proportion of neoformation were L (0.39% ± 0.13) and C (0.37% ± 0.97), but groups B and B+L had larger defect size (C-1.75 mm2 ± 0.40, B-3.02 mm2 ± 0.63, L-2.45 mm2 ± 0.53, B+L-3.23 mm2 ± 1.01). In immunohistochemistry, groups B and B+L had higher immunostaining scores for OPG and RANKL at 60 days, and TRAP immunostaining increased in all groups at 30 days, but group L was the only one to present specimens with score 0. Although, at 60 days, groups L and C presented the highest proportion of bone neoformation, at 30 days group B+L had more than twice as much bone neoformation as group B, the choice of treatment application should depend on the aim of the treatment. [ABSTRACT FROM AUTHOR] |
| Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Items | – Name: Title Label: Title Group: Ti Data: Bone repair assessment of critical size defects in rats treated with mineralized bovine bone (Bio-Oss®) and photobiomodulation therapy: a histomorphometric and immunohistochemical study. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Torquato%2C+Letícia+Cavassini%22">Torquato, Letícia Cavassini</searchLink><relatesTo>1</relatesTo><i> leticia.c.torquato@hotmail.com</i><br /><searchLink fieldCode="AR" term="%22Suárez%2C+Eduardo+Antonio+Chelin%22">Suárez, Eduardo Antonio Chelin</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Bernardo%2C+Daniella+Viscensotto%22">Bernardo, Daniella Viscensotto</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Pinto%2C+Isis+Luzcybel+Ribeiro%22">Pinto, Isis Luzcybel Ribeiro</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Mantovani%2C+Ludmilla+Oliveira%22">Mantovani, Ludmilla Oliveira</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Silva%2C+Thiago+Igor+Lemes%22">Silva, Thiago Igor Lemes</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Jardini%2C+Maria+Aparecida+Neves%22">Jardini, Maria Aparecida Neves</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Santamaria%2C+Mauro+Pedrine%22">Santamaria, Mauro Pedrine</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22De+Marco%2C+Andrea+Carvalho%22">De Marco, Andrea Carvalho</searchLink><relatesTo>3</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Lasers+in+Medical+Science%22">Lasers in Medical Science</searchLink>. Sep2021, Vol. 36 Issue 7, p1515-1525. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Photodynamic+therapy%22">Photodynamic therapy</searchLink><br /><searchLink fieldCode="DE" term="%22Osteoprotegerin%22">Osteoprotegerin</searchLink><br /><searchLink fieldCode="DE" term="%22Immunohistochemistry%22">Immunohistochemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Bone+regeneration%22">Bone regeneration</searchLink><br /><searchLink fieldCode="DE" term="%22Immunostaining%22">Immunostaining</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: This study aimed to investigate the effects of administering photobiomodulation therapy (PBM) with bovine bone matrix on critical size defects in rats. Seventy-two adult male rats (albinus, Wistar), 90 days old, were used. Defect of 5 mm in diameter was made in their calvaria. The animals were divided into 4 groups: C-blood clot, B-Bio-Oss®, L-PBM, B+L-Bio-Oss®+PBM. Each group has been subdivided into 07, 30, and 60 days of observation. For PBM, a low GaAlAs energy of 660 nm was irradiated, total energy density of 45 J/cm2. PBM was conducted in a trans-surgical form once only. For immunohistochemistry, a semi-quantitative analysis was made of expression of osteoprotegerin (OPG), nuclear kappa B-factor ligand receptor activator (RANKL), and tartrate-resistant acid phosphatase (TRAP). All histomorphometric data were statistically analyzed by ANOVA and Tukey test, significance level of 5%. The groups that showed the highest proportion of neoformation were L (0.39% ± 0.13) and C (0.37% ± 0.97), but groups B and B+L had larger defect size (C-1.75 mm2 ± 0.40, B-3.02 mm2 ± 0.63, L-2.45 mm2 ± 0.53, B+L-3.23 mm2 ± 1.01). In immunohistochemistry, groups B and B+L had higher immunostaining scores for OPG and RANKL at 60 days, and TRAP immunostaining increased in all groups at 30 days, but group L was the only one to present specimens with score 0. Although, at 60 days, groups L and C presented the highest proportion of bone neoformation, at 30 days group B+L had more than twice as much bone neoformation as group B, the choice of treatment application should depend on the aim of the treatment. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=151838874 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s10103-020-03234-5 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 1515 Subjects: – SubjectFull: Photodynamic therapy Type: general – SubjectFull: Osteoprotegerin Type: general – SubjectFull: Immunohistochemistry Type: general – SubjectFull: Bone regeneration Type: general – SubjectFull: Immunostaining Type: general Titles: – TitleFull: Bone repair assessment of critical size defects in rats treated with mineralized bovine bone (Bio-Oss®) and photobiomodulation therapy: a histomorphometric and immunohistochemical study. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Torquato, Letícia Cavassini – PersonEntity: Name: NameFull: Suárez, Eduardo Antonio Chelin – PersonEntity: Name: NameFull: Bernardo, Daniella Viscensotto – PersonEntity: Name: NameFull: Pinto, Isis Luzcybel Ribeiro – PersonEntity: Name: NameFull: Mantovani, Ludmilla Oliveira – PersonEntity: Name: NameFull: Silva, Thiago Igor Lemes – PersonEntity: Name: NameFull: Jardini, Maria Aparecida Neves – PersonEntity: Name: NameFull: Santamaria, Mauro Pedrine – PersonEntity: Name: NameFull: De Marco, Andrea Carvalho IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: Sep2021 Type: published Y: 2021 Identifiers: – Type: issn-print Value: 02688921 Numbering: – Type: volume Value: 36 – Type: issue Value: 7 Titles: – TitleFull: Lasers in Medical Science Type: main |
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