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Genomic stability inArabidopsis thalianatransgenic plants obtained by floral dip.

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Title: Genomic stability inArabidopsis thalianatransgenic plants obtained by floral dip.
Authors: Labra, M.1 massimo.labra@unimib.it, Vannini, C.2, Grassi, F.3, Bracale, M.2, Balsemin, M.2, Basso, B.4, Sala, F.3,4
Source: Theoretical & Applied Genetics. Nov2004, Vol. 109 Issue 7, p1512-1518. 7p.
Subjects: Arabidopsis thaliana, Arabidopsis, Transgenic plants, Plant genetic engineering, Plant biotechnology, Plant genetics, Genetics
Abstract: The occurrence of DNA modification is an undesired phenomenon accompanying plant cell transformation. The event has been correlated with the stress imposed by the presently utilised transformation procedures, all depending on plant differentiation from in vitro cell culture, but other causes have not been excluded. In this work, transgenicArabidopsis thalianaplants have been produced by an approach that does not require cell dedifferentiation, being based onin plantaAgrobacterium-mediated gene transfer by flower infiltration, which is followed by recovery and selection of transgenic progeny. Genomic DNA changes in transgenic and control plants have been investigated by AFLP and RAMP analysis. Results show no statistically relevant genomic modifications in transgenic plants, as compared with control untreated plants. Variations were observed in callus-derivedA. thalianaplants, thus supporting the conclusion that somaclonal variation is essentially correlated with the stress imposed by the in vitro cell culture, rather than with the integration of a foreign gene. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:The occurrence of DNA modification is an undesired phenomenon accompanying plant cell transformation. The event has been correlated with the stress imposed by the presently utilised transformation procedures, all depending on plant differentiation from in vitro cell culture, but other causes have not been excluded. In this work, transgenicArabidopsis thalianaplants have been produced by an approach that does not require cell dedifferentiation, being based onin plantaAgrobacterium-mediated gene transfer by flower infiltration, which is followed by recovery and selection of transgenic progeny. Genomic DNA changes in transgenic and control plants have been investigated by AFLP and RAMP analysis. Results show no statistically relevant genomic modifications in transgenic plants, as compared with control untreated plants. Variations were observed in callus-derivedA. thalianaplants, thus supporting the conclusion that somaclonal variation is essentially correlated with the stress imposed by the in vitro cell culture, rather than with the integration of a foreign gene. [ABSTRACT FROM AUTHOR]
ISSN:00405752
DOI:10.1007/s00122-004-1773-y