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Genomic stability inArabidopsis thalianatransgenic plants obtained by floral dip.

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Title: Genomic stability inArabidopsis thalianatransgenic plants obtained by floral dip.
Authors: Labra, M.1 massimo.labra@unimib.it, Vannini, C.2, Grassi, F.3, Bracale, M.2, Balsemin, M.2, Basso, B.4, Sala, F.3,4
Source: Theoretical & Applied Genetics. Nov2004, Vol. 109 Issue 7, p1512-1518. 7p.
Subjects: Arabidopsis thaliana, Arabidopsis, Transgenic plants, Plant genetic engineering, Plant biotechnology, Plant genetics, Genetics
Abstract: The occurrence of DNA modification is an undesired phenomenon accompanying plant cell transformation. The event has been correlated with the stress imposed by the presently utilised transformation procedures, all depending on plant differentiation from in vitro cell culture, but other causes have not been excluded. In this work, transgenicArabidopsis thalianaplants have been produced by an approach that does not require cell dedifferentiation, being based onin plantaAgrobacterium-mediated gene transfer by flower infiltration, which is followed by recovery and selection of transgenic progeny. Genomic DNA changes in transgenic and control plants have been investigated by AFLP and RAMP analysis. Results show no statistically relevant genomic modifications in transgenic plants, as compared with control untreated plants. Variations were observed in callus-derivedA. thalianaplants, thus supporting the conclusion that somaclonal variation is essentially correlated with the stress imposed by the in vitro cell culture, rather than with the integration of a foreign gene. [ABSTRACT FROM AUTHOR]
Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: <searchLink fieldCode="DE" term="%22Arabidopsis+thaliana%22">Arabidopsis thaliana</searchLink><br /><searchLink fieldCode="DE" term="%22Arabidopsis%22">Arabidopsis</searchLink><br /><searchLink fieldCode="DE" term="%22Transgenic+plants%22">Transgenic plants</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+genetic+engineering%22">Plant genetic engineering</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+biotechnology%22">Plant biotechnology</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+genetics%22">Plant genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Genetics%22">Genetics</searchLink>
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  Data: The occurrence of DNA modification is an undesired phenomenon accompanying plant cell transformation. The event has been correlated with the stress imposed by the presently utilised transformation procedures, all depending on plant differentiation from in vitro cell culture, but other causes have not been excluded. In this work, transgenicArabidopsis thalianaplants have been produced by an approach that does not require cell dedifferentiation, being based onin plantaAgrobacterium-mediated gene transfer by flower infiltration, which is followed by recovery and selection of transgenic progeny. Genomic DNA changes in transgenic and control plants have been investigated by AFLP and RAMP analysis. Results show no statistically relevant genomic modifications in transgenic plants, as compared with control untreated plants. Variations were observed in callus-derivedA. thalianaplants, thus supporting the conclusion that somaclonal variation is essentially correlated with the stress imposed by the in vitro cell culture, rather than with the integration of a foreign gene. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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