Effect of devitrification on the survival and resistance of dried Saccharomyces cerevisiae yeast.
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| Title: | Effect of devitrification on the survival and resistance of dried Saccharomyces cerevisiae yeast. |
|---|---|
| Authors: | Ribert, Pauline1,2 (AUTHOR), Dupont, Sébastien1 (AUTHOR), Roudaut, Gaëlle1 (AUTHOR), Beney, Laurent1 (AUTHOR) lbeney@u-bourgogne.fr |
| Source: | Applied Microbiology & Biotechnology. Aug2021, Vol. 105 Issue 16/17, p6409-6418. 10p. |
| Subjects: | Devitrification, Saccharomyces cerevisiae, Glass transition temperature, Yeast, Survival rate |
| Abstract: | Yeasts are anhydrobiotes that accumulate large amounts of trehalose, which is involved in the vitrification of the cytoplasm during drastic desiccation. The effect of devitrification, which can be induced by the transient exposure of desiccated yeasts to increased humidity or elevated temperature, on the survival of yeast has been studied. A glass transition temperature (Tg)/water activity (aw) diagram of yeast was constructed based on differential scanning calorimetry analysis. The survival rate of yeasts that were equilibrated at different relative humidities (RHs) and temperature values over their Tg range was measured. The results revealed a long period of cell preservation at an intermediate RH (55%), with 100% survival observed after 3 months, a loss of 1.24 log colony-forming units/g recorded after 1 year at 25 °C and full preservation of viability at 75 °C for 60 min and at 100 °C and 12% RH for up to 10 min. These findings led us to conclude that dried yeast can resist low or intermediate RH values and elevated temperatures in the devitrified state. Considering the thermal and humidity fluctuations occurring in the yeast environments, we hypothesized that the supercooled state, which occurs immediately above the Tg after rehydration or heating, is a protective state that is involved in the persistence of yeasts at intermediate humidity levels. Key points: • Yeast survival for months in a supercooled state is observed at room temperature. • Dried yeasts survive a 10-min exposure to 100 °C in the supercooled state. • The supercooled state is suitable for yeast preservation. [ABSTRACT FROM AUTHOR] |
| Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Items | – Name: Title Label: Title Group: Ti Data: Effect of devitrification on the survival and resistance of dried Saccharomyces cerevisiae yeast. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Ribert%2C+Pauline%22">Ribert, Pauline</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Dupont%2C+Sébastien%22">Dupont, Sébastien</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Roudaut%2C+Gaëlle%22">Roudaut, Gaëlle</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Beney%2C+Laurent%22">Beney, Laurent</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> lbeney@u-bourgogne.fr</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. Aug2021, Vol. 105 Issue 16/17, p6409-6418. 10p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Devitrification%22">Devitrification</searchLink><br /><searchLink fieldCode="DE" term="%22Saccharomyces+cerevisiae%22">Saccharomyces cerevisiae</searchLink><br /><searchLink fieldCode="DE" term="%22Glass+transition+temperature%22">Glass transition temperature</searchLink><br /><searchLink fieldCode="DE" term="%22Yeast%22">Yeast</searchLink><br /><searchLink fieldCode="DE" term="%22Survival+rate%22">Survival rate</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Yeasts are anhydrobiotes that accumulate large amounts of trehalose, which is involved in the vitrification of the cytoplasm during drastic desiccation. The effect of devitrification, which can be induced by the transient exposure of desiccated yeasts to increased humidity or elevated temperature, on the survival of yeast has been studied. A glass transition temperature (Tg)/water activity (aw) diagram of yeast was constructed based on differential scanning calorimetry analysis. The survival rate of yeasts that were equilibrated at different relative humidities (RHs) and temperature values over their Tg range was measured. The results revealed a long period of cell preservation at an intermediate RH (55%), with 100% survival observed after 3 months, a loss of 1.24 log colony-forming units/g recorded after 1 year at 25 °C and full preservation of viability at 75 °C for 60 min and at 100 °C and 12% RH for up to 10 min. These findings led us to conclude that dried yeast can resist low or intermediate RH values and elevated temperatures in the devitrified state. Considering the thermal and humidity fluctuations occurring in the yeast environments, we hypothesized that the supercooled state, which occurs immediately above the Tg after rehydration or heating, is a protective state that is involved in the persistence of yeasts at intermediate humidity levels. Key points: • Yeast survival for months in a supercooled state is observed at room temperature. • Dried yeasts survive a 10-min exposure to 100 °C in the supercooled state. • The supercooled state is suitable for yeast preservation. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00253-021-11451-3 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 6409 Subjects: – SubjectFull: Devitrification Type: general – SubjectFull: Saccharomyces cerevisiae Type: general – SubjectFull: Glass transition temperature Type: general – SubjectFull: Yeast Type: general – SubjectFull: Survival rate Type: general Titles: – TitleFull: Effect of devitrification on the survival and resistance of dried Saccharomyces cerevisiae yeast. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Ribert, Pauline – PersonEntity: Name: NameFull: Dupont, Sébastien – PersonEntity: Name: NameFull: Roudaut, Gaëlle – PersonEntity: Name: NameFull: Beney, Laurent IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 08 Text: Aug2021 Type: published Y: 2021 Identifiers: – Type: issn-print Value: 01757598 Numbering: – Type: volume Value: 105 – Type: issue Value: 16/17 Titles: – TitleFull: Applied Microbiology & Biotechnology Type: main |
| ResultId | 1 |
