Efficacy and Metagenomic Analysis of the Stabilized Anaerobic Lignocellulolytic Microbial Consortium from Bubalus bubalis Rumen with Rice Straw Enrichment for Methane Production.
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| Title: | Efficacy and Metagenomic Analysis of the Stabilized Anaerobic Lignocellulolytic Microbial Consortium from Bubalus bubalis Rumen with Rice Straw Enrichment for Methane Production. |
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| Authors: | Thongbunrod, Nitiya1 (AUTHOR), Chaiprasert, Pawinee1 (AUTHOR) pawinee.cha@kmutt.ac.th |
| Source: | BioEnergy Research. Sep2021, Vol. 14 Issue 3, p870-890. 21p. |
| Subjects: | Rice straw, Metagenomics, Cattle manure, Methane, Shotgun sequencing, Water buffalo, Goats, Hemicellulose |
| Abstract: | Five different inocula from cow manure, goat manure, pig manure, and rumen fluid (RU) and microbial sludge from anaerobic digestion were screened to select anaerobic lignocellulolytic microbial consortium (ALMC) of high efficacy in degradation of filter paper (FP), microcrystalline cellulose (MCC), and rice straw (RS) to produce methane. RU from Bubalus bubalis demonstrated the highest growth rate RU inoculum (10.45 mgVS/day); fastest degradation rate of FP, MCC, and RS (125.00, 50.07, and 25.43 mgVS/day); and highest methane production rate (6.23 ml/day). To obtain the enriched and stabilized ALMC from RU, consecutive batch subculturing was used and reached at 25 passages, namely, E25. The efficacies of E25 in RS removal and methane production were 78%VS and 293 ml/gVSadded compared with initial inoculum (E0) at 46% VS and 138 ml/gVSadded, respectively, within 14 days of incubation time. The efficacies of E25 in RS removal and methane production were 1.7- and 2.12-folds higher than of E0, respectively. E25 showed high ability to utilize various carbon sources (68%) from Biolog EcoPlate assay. It was confirmed that E25 could degrade derivatives of cellulose, hemicellulose, and lignin. Metagenomic analysis by 16 s and shotgun sequencing of E25 found dominant species of hydrolytic/fermentative bacteria (Proteiniphilum acetatigenes, Pyramidobacter piscolens, Syntrophus aciditrophicus, Mesotoga prima, and Candidatus Cloacimonas acidaminovorans) and methanogens (Methanoculleus bourgensis, Methanoculleus marisnigri, Methanofollis liminatans, Methanosarcina mazei, and Methanosaeta harundinacea). This stabilized E25 with enrichment of lignocellulolytic and fermentative bacteria as well as hydrogenotrophic and acetoclastic methanogens shows its efficacy in accelerating RS degradation and methane production. [ABSTRACT FROM AUTHOR] |
| Copyright of BioEnergy Research is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Items | – Name: Title Label: Title Group: Ti Data: Efficacy and Metagenomic Analysis of the Stabilized Anaerobic Lignocellulolytic Microbial Consortium from Bubalus bubalis Rumen with Rice Straw Enrichment for Methane Production. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Thongbunrod%2C+Nitiya%22">Thongbunrod, Nitiya</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chaiprasert%2C+Pawinee%22">Chaiprasert, Pawinee</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> pawinee.cha@kmutt.ac.th</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22BioEnergy+Research%22">BioEnergy Research</searchLink>. Sep2021, Vol. 14 Issue 3, p870-890. 21p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Rice+straw%22">Rice straw</searchLink><br /><searchLink fieldCode="DE" term="%22Metagenomics%22">Metagenomics</searchLink><br /><searchLink fieldCode="DE" term="%22Cattle+manure%22">Cattle manure</searchLink><br /><searchLink fieldCode="DE" term="%22Methane%22">Methane</searchLink><br /><searchLink fieldCode="DE" term="%22Shotgun+sequencing%22">Shotgun sequencing</searchLink><br /><searchLink fieldCode="DE" term="%22Water+buffalo%22">Water buffalo</searchLink><br /><searchLink fieldCode="DE" term="%22Goats%22">Goats</searchLink><br /><searchLink fieldCode="DE" term="%22Hemicellulose%22">Hemicellulose</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Five different inocula from cow manure, goat manure, pig manure, and rumen fluid (RU) and microbial sludge from anaerobic digestion were screened to select anaerobic lignocellulolytic microbial consortium (ALMC) of high efficacy in degradation of filter paper (FP), microcrystalline cellulose (MCC), and rice straw (RS) to produce methane. RU from Bubalus bubalis demonstrated the highest growth rate RU inoculum (10.45 mgVS/day); fastest degradation rate of FP, MCC, and RS (125.00, 50.07, and 25.43 mgVS/day); and highest methane production rate (6.23 ml/day). To obtain the enriched and stabilized ALMC from RU, consecutive batch subculturing was used and reached at 25 passages, namely, E25. The efficacies of E25 in RS removal and methane production were 78%VS and 293 ml/gVSadded compared with initial inoculum (E0) at 46% VS and 138 ml/gVSadded, respectively, within 14 days of incubation time. The efficacies of E25 in RS removal and methane production were 1.7- and 2.12-folds higher than of E0, respectively. E25 showed high ability to utilize various carbon sources (68%) from Biolog EcoPlate assay. It was confirmed that E25 could degrade derivatives of cellulose, hemicellulose, and lignin. Metagenomic analysis by 16 s and shotgun sequencing of E25 found dominant species of hydrolytic/fermentative bacteria (Proteiniphilum acetatigenes, Pyramidobacter piscolens, Syntrophus aciditrophicus, Mesotoga prima, and Candidatus Cloacimonas acidaminovorans) and methanogens (Methanoculleus bourgensis, Methanoculleus marisnigri, Methanofollis liminatans, Methanosarcina mazei, and Methanosaeta harundinacea). This stabilized E25 with enrichment of lignocellulolytic and fermentative bacteria as well as hydrogenotrophic and acetoclastic methanogens shows its efficacy in accelerating RS degradation and methane production. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of BioEnergy Research is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s12155-020-10167-y Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 21 StartPage: 870 Subjects: – SubjectFull: Rice straw Type: general – SubjectFull: Metagenomics Type: general – SubjectFull: Cattle manure Type: general – SubjectFull: Methane Type: general – SubjectFull: Shotgun sequencing Type: general – SubjectFull: Water buffalo Type: general – SubjectFull: Goats Type: general – SubjectFull: Hemicellulose Type: general Titles: – TitleFull: Efficacy and Metagenomic Analysis of the Stabilized Anaerobic Lignocellulolytic Microbial Consortium from Bubalus bubalis Rumen with Rice Straw Enrichment for Methane Production. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Thongbunrod, Nitiya – PersonEntity: Name: NameFull: Chaiprasert, Pawinee IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: Sep2021 Type: published Y: 2021 Identifiers: – Type: issn-print Value: 19391234 Numbering: – Type: volume Value: 14 – Type: issue Value: 3 Titles: – TitleFull: BioEnergy Research Type: main |
| ResultId | 1 |
