Anchoring alien chromosome segment substitutions bearing gene(s) for resistance to mustard aphid in Brassica juncea-B. fruticulosa introgression lines and their possible disruption through gamma irradiation.
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| Title: | Anchoring alien chromosome segment substitutions bearing gene(s) for resistance to mustard aphid in Brassica juncea-B. fruticulosa introgression lines and their possible disruption through gamma irradiation. |
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| Authors: | Agrawal, Neha1 (AUTHOR), Gupta, Mehak1 (AUTHOR), Atri, Chhaya1 (AUTHOR), Akhatar, Javed1 (AUTHOR), Kumar, Sarwan1 (AUTHOR), Heslop-Harrison, Pat2 (AUTHOR), Banga, Surinder S.1 (AUTHOR) nppbg@pau.edu |
| Source: | Theoretical & Applied Genetics. Oct2021, Vol. 134 Issue 10, p3209-3224. 16p. |
| Subjects: | Brassica juncea, Chromosomes, Mustard, Nucleic acid probes, Brassica, Aphids |
| Abstract: | Key message: Heavy doses of gamma irradiation can reduce linkage drag by disrupting large sized alien translocations and promoting exchanges between crop and wild genomes. Resistance to mustard aphid (Lipaphis erysimi) infestation was significantly improved in Brassica juncea through B. juncea-B. fruticulosa introgression. However, linkage drag caused by introgressed chromatin fragments has so far prevented the deployment of this resistance source in commercial cultivars. We investigated the patterns of donor chromatin segment substitutions in the introgression lines (ILs) through genomic in situ hybridization (GISH) coupled with B. juncea chromosome-specific oligonucleotide probes. These allowed identification of large chromosome translocations from B. fruticulosa in the terminal regions of chromosomes A05, B02, B03 and B04 in three founder ILs (AD-64, 101 and 104). Only AD-101 carried an additional translocation at the sub-terminal to intercalary position in both homologues of chromosome A01. We validated these translocations with a reciprocal blast hit analysis using shotgun sequencing of three ILs and species-specific contigs/scaffolds (kb sized) from a de novo assembly of B. fruticulosa. Alien segment substitution on chromosome A05 could not be validated. Current studies also endeavoured to break linkage drag by exposing seeds to a heavy dose (200kR) of gamma radiation. Reduction in the size of introgressed chromatin fragments was observed in many M3 plants. There was a complete loss of the alien chromosome fragment in one instance. A few M3 plants with novel patterns of chromosome segment substitutions displayed improved agronomic performance coupled with resistance to mustard aphid. SNPs in such genomic spaces should aid the development of markers to track introgressed DNA and allow application in plant breeding. [ABSTRACT FROM AUTHOR] |
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| Items | – Name: Title Label: Title Group: Ti Data: Anchoring alien chromosome segment substitutions bearing gene(s) for resistance to mustard aphid in Brassica juncea-B. fruticulosa introgression lines and their possible disruption through gamma irradiation. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Agrawal%2C+Neha%22">Agrawal, Neha</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gupta%2C+Mehak%22">Gupta, Mehak</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Atri%2C+Chhaya%22">Atri, Chhaya</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Akhatar%2C+Javed%22">Akhatar, Javed</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kumar%2C+Sarwan%22">Kumar, Sarwan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Heslop-Harrison%2C+Pat%22">Heslop-Harrison, Pat</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Banga%2C+Surinder+S%2E%22">Banga, Surinder S.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> nppbg@pau.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. Oct2021, Vol. 134 Issue 10, p3209-3224. 16p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Brassica+juncea%22">Brassica juncea</searchLink><br /><searchLink fieldCode="DE" term="%22Chromosomes%22">Chromosomes</searchLink><br /><searchLink fieldCode="DE" term="%22Mustard%22">Mustard</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleic+acid+probes%22">Nucleic acid probes</searchLink><br /><searchLink fieldCode="DE" term="%22Brassica%22">Brassica</searchLink><br /><searchLink fieldCode="DE" term="%22Aphids%22">Aphids</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Key message: Heavy doses of gamma irradiation can reduce linkage drag by disrupting large sized alien translocations and promoting exchanges between crop and wild genomes. Resistance to mustard aphid (Lipaphis erysimi) infestation was significantly improved in Brassica juncea through B. juncea-B. fruticulosa introgression. However, linkage drag caused by introgressed chromatin fragments has so far prevented the deployment of this resistance source in commercial cultivars. We investigated the patterns of donor chromatin segment substitutions in the introgression lines (ILs) through genomic in situ hybridization (GISH) coupled with B. juncea chromosome-specific oligonucleotide probes. These allowed identification of large chromosome translocations from B. fruticulosa in the terminal regions of chromosomes A05, B02, B03 and B04 in three founder ILs (AD-64, 101 and 104). Only AD-101 carried an additional translocation at the sub-terminal to intercalary position in both homologues of chromosome A01. We validated these translocations with a reciprocal blast hit analysis using shotgun sequencing of three ILs and species-specific contigs/scaffolds (kb sized) from a de novo assembly of B. fruticulosa. Alien segment substitution on chromosome A05 could not be validated. Current studies also endeavoured to break linkage drag by exposing seeds to a heavy dose (200kR) of gamma radiation. Reduction in the size of introgressed chromatin fragments was observed in many M3 plants. There was a complete loss of the alien chromosome fragment in one instance. A few M3 plants with novel patterns of chromosome segment substitutions displayed improved agronomic performance coupled with resistance to mustard aphid. SNPs in such genomic spaces should aid the development of markers to track introgressed DNA and allow application in plant breeding. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00122-021-03886-z Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 16 StartPage: 3209 Subjects: – SubjectFull: Brassica juncea Type: general – SubjectFull: Chromosomes Type: general – SubjectFull: Mustard Type: general – SubjectFull: Nucleic acid probes Type: general – SubjectFull: Brassica Type: general – SubjectFull: Aphids Type: general Titles: – TitleFull: Anchoring alien chromosome segment substitutions bearing gene(s) for resistance to mustard aphid in Brassica juncea-B. fruticulosa introgression lines and their possible disruption through gamma irradiation. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Agrawal, Neha – PersonEntity: Name: NameFull: Gupta, Mehak – PersonEntity: Name: NameFull: Atri, Chhaya – PersonEntity: Name: NameFull: Akhatar, Javed – PersonEntity: Name: NameFull: Kumar, Sarwan – PersonEntity: Name: NameFull: Heslop-Harrison, Pat – PersonEntity: Name: NameFull: Banga, Surinder S. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 10 Text: Oct2021 Type: published Y: 2021 Identifiers: – Type: issn-print Value: 00405752 Numbering: – Type: volume Value: 134 – Type: issue Value: 10 Titles: – TitleFull: Theoretical & Applied Genetics Type: main |
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