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Using a COI mini‐barcode and real‐time PCR (qPCR) for sea turtle identification in processed food.

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Title: Using a COI mini‐barcode and real‐time PCR (qPCR) for sea turtle identification in processed food.
Authors: Villanueva‐Zayas, Jesús Daniel1 (AUTHOR), Rodríguez‐Ramírez, Roberto1 (AUTHOR) roberto.rodriguez@itson.edu.mx, Ávila‐Villa, Luz Angélica2 (AUTHOR), González‐Córdova, Aarón F.3 (AUTHOR), Reyes‐López, Miguel Ángel4 (AUTHOR), Hernández‐Sierra, Daniel1 (AUTHOR), de los Santos‐Villalobos, Sergio1 (AUTHOR)
Source: Journal of Food Processing & Preservation. Oct2021, Vol. 45 Issue 10, p1-10. 10p.
Subjects: Sea turtles, Polymerase chain reaction, Endangered species, Protein structure, DNA structure
Geographic Terms: Mexico
Abstract: The worldwide regulations protect sea turtles, nevertheless, the illicit consumption of their by‐products keeps them as endangered species. In Mexico, its meat is used illegally to prepare food and in recent years this has caused a huge problem for control agencies. Analytical methods used for species detection in food are affected due to changes in the protein structure or degradation of DNA after heat treatment. The present work aimed to use a mini‐barcode based on the COI gene (MBCOI) and real‐time polymerase chain reaction (qPCR) for sea turtle identification in processed foods. The MBCOI‐qPCR showed high specificity without any cross‐reactions, the limit of detection was 1 pg using processed turtle DNA with a PCR efficiency of 101.9%. This study demonstrated the ability to estimate up to 1% of processed sea turtles, which could be considered as a tool to stop the illegal trade and consumption of sea turtles. Novelty impact statement: Sea turtles are endangered species however their meat is consumed illegally in Mexico in a dish known as sea turtle soup. This study presents the development of a novel method combining a mini‐barcode and real‐time polymerase chain reaction capable to detect and estimate sea turtles in processed foods. Our results suggest that the method described herein could be an important tool to detect those involved in the illegal consumption and protect sea turtle species. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Food Processing & Preservation is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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PubTypeId: academicJournal
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  Data: Using a COI mini‐barcode and real‐time PCR (qPCR) for sea turtle identification in processed food.
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  Data: <searchLink fieldCode="AR" term="%22Villanueva‐Zayas%2C+Jesús+Daniel%22">Villanueva‐Zayas, Jesús Daniel</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Rodríguez‐Ramírez%2C+Roberto%22">Rodríguez‐Ramírez, Roberto</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> roberto.rodriguez@itson.edu.mx</i><br /><searchLink fieldCode="AR" term="%22Ávila‐Villa%2C+Luz+Angélica%22">Ávila‐Villa, Luz Angélica</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22González‐Córdova%2C+Aarón+F%2E%22">González‐Córdova, Aarón F.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Reyes‐López%2C+Miguel+Ángel%22">Reyes‐López, Miguel Ángel</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hernández‐Sierra%2C+Daniel%22">Hernández‐Sierra, Daniel</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22de+los+Santos‐Villalobos%2C+Sergio%22">de los Santos‐Villalobos, Sergio</searchLink><relatesTo>1</relatesTo> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Food+Processing+%26+Preservation%22">Journal of Food Processing & Preservation</searchLink>. Oct2021, Vol. 45 Issue 10, p1-10. 10p.
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  Data: <searchLink fieldCode="DE" term="%22Sea+turtles%22">Sea turtles</searchLink><br /><searchLink fieldCode="DE" term="%22Polymerase+chain+reaction%22">Polymerase chain reaction</searchLink><br /><searchLink fieldCode="DE" term="%22Endangered+species%22">Endangered species</searchLink><br /><searchLink fieldCode="DE" term="%22Protein+structure%22">Protein structure</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+structure%22">DNA structure</searchLink>
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  Data: <searchLink fieldCode="DE" term="%22Mexico%22">Mexico</searchLink>
– Name: Abstract
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  Data: The worldwide regulations protect sea turtles, nevertheless, the illicit consumption of their by‐products keeps them as endangered species. In Mexico, its meat is used illegally to prepare food and in recent years this has caused a huge problem for control agencies. Analytical methods used for species detection in food are affected due to changes in the protein structure or degradation of DNA after heat treatment. The present work aimed to use a mini‐barcode based on the COI gene (MBCOI) and real‐time polymerase chain reaction (qPCR) for sea turtle identification in processed foods. The MBCOI‐qPCR showed high specificity without any cross‐reactions, the limit of detection was 1 pg using processed turtle DNA with a PCR efficiency of 101.9%. This study demonstrated the ability to estimate up to 1% of processed sea turtles, which could be considered as a tool to stop the illegal trade and consumption of sea turtles. Novelty impact statement: Sea turtles are endangered species however their meat is consumed illegally in Mexico in a dish known as sea turtle soup. This study presents the development of a novel method combining a mini‐barcode and real‐time polymerase chain reaction capable to detect and estimate sea turtles in processed foods. Our results suggest that the method described herein could be an important tool to detect those involved in the illegal consumption and protect sea turtle species. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Journal of Food Processing & Preservation is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1111/jfpp.15808
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        Text: English
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      – SubjectFull: Sea turtles
        Type: general
      – SubjectFull: Polymerase chain reaction
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      – SubjectFull: Endangered species
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      – SubjectFull: Protein structure
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      – SubjectFull: DNA structure
        Type: general
      – SubjectFull: Mexico
        Type: general
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      – TitleFull: Using a COI mini‐barcode and real‐time PCR (qPCR) for sea turtle identification in processed food.
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            NameFull: Villanueva‐Zayas, Jesús Daniel
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            – D: 01
              M: 10
              Text: Oct2021
              Type: published
              Y: 2021
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