Exendin-4-Conjugated Manganese Magnetism-Engineered Iron Oxide Nanoparticles as a Potential Magnetic Resonance Imaging Contrast Agent for Tracking Transplanted β-Cells.
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| Title: | Exendin-4-Conjugated Manganese Magnetism-Engineered Iron Oxide Nanoparticles as a Potential Magnetic Resonance Imaging Contrast Agent for Tracking Transplanted β-Cells. |
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| Authors: | Juang, Jyuhn-Huarng1,2 (AUTHOR) Je3474@gmail.com, Shen, Chia-Rui3,4 (AUTHOR) crshen@mail.cgu.edu.tw, Wang, Jiun-Jie5,6 (AUTHOR) jiunjie.wang@gmail.com, Wu, Shu-Ting3 (AUTHOR) proteinwhite@livemail.tw, Lin, Sung-Han5 (AUTHOR) image.lin@gmail.com, Chen, Chen-Yi1 (AUTHOR) lian8807111@gmail.com, Kao, Chen-Wei1 (AUTHOR) jenny74513@gmail.com, Chen, Chen-Ling1 (AUTHOR), Tsai, Zei-Tsan7 (AUTHOR) zeitsan@ms9.hinet.net, Wang, Yun-Ming8 (AUTHOR) jjuang@cgmh.org.tw |
| Source: | Nanomaterials (2079-4991). Nov2021, Vol. 11 Issue 11, p3145-3145. 1p. |
| Subjects: | Iron oxide nanoparticles, Iron oxides, Magnetic resonance imaging, Contrast media, Magnetic nanoparticles, Manganese, Islands of Langerhans, Glucagon-like peptide-1 receptor |
| Abstract: | To specifically detect and trace transplanted islet β-cells by magnetic resonance imaging (MRI), we conjugated manganese magnetism-engineered iron oxide nanoparticles (MnMEIO NPs) with exendin-4 (Ex4) which specifically binds glucagon-like peptide-1 receptors on the surface of β-cells. The size distribution of MnMEIO and MnMEIO-Ex4 NPs were 67.8 ± 1.3 and 70.2 ± 2.3 nm and zeta potential 33.3 ± 0.5 and 0.6 ± 0.1 mV, respectively. MnMEIO and MnMEIO-Ex4 NPs with iron content ≤ 40 μg/mL did not affect MIN6 β-cell viability and insulin secretion. Positive iron staining was found in MIN6 β-cells loaded with MnMEIO-Ex4 NPs but not in those with MnMEIO NPs. A transmission electron microscope confirmed MnMEIO-Ex4 NPs were distributed in the cytoplasm of MIN6. In vitro MR images revealed a loss of signal intensity in MIN6 β-cells labeled with MnMEIO-Ex4 NPs but not with MnMEIO NPs. After transplantation of islets labeled with MnMEIO-Ex4, the graft under kidney capsule could be visualized on MRI as persistent hypointense areas up to 17 weeks. Moreover, histology of the islet graft showed positive staining for insulin, glucagon and iron. Our results indicate MnMEIO-Ex4 NPs are safe and effective for the detection and long-term monitoring of transplanted β-cells by MRI. [ABSTRACT FROM AUTHOR] |
| Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Items | – Name: Title Label: Title Group: Ti Data: Exendin-4-Conjugated Manganese Magnetism-Engineered Iron Oxide Nanoparticles as a Potential Magnetic Resonance Imaging Contrast Agent for Tracking Transplanted β-Cells. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Juang%2C+Jyuhn-Huarng%22">Juang, Jyuhn-Huarng</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> Je3474@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Shen%2C+Chia-Rui%22">Shen, Chia-Rui</searchLink><relatesTo>3,4</relatesTo> (AUTHOR)<i> crshen@mail.cgu.edu.tw</i><br /><searchLink fieldCode="AR" term="%22Wang%2C+Jiun-Jie%22">Wang, Jiun-Jie</searchLink><relatesTo>5,6</relatesTo> (AUTHOR)<i> jiunjie.wang@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Wu%2C+Shu-Ting%22">Wu, Shu-Ting</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> proteinwhite@livemail.tw</i><br /><searchLink fieldCode="AR" term="%22Lin%2C+Sung-Han%22">Lin, Sung-Han</searchLink><relatesTo>5</relatesTo> (AUTHOR)<i> image.lin@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Chen%2C+Chen-Yi%22">Chen, Chen-Yi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> lian8807111@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Kao%2C+Chen-Wei%22">Kao, Chen-Wei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> jenny74513@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Chen%2C+Chen-Ling%22">Chen, Chen-Ling</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tsai%2C+Zei-Tsan%22">Tsai, Zei-Tsan</searchLink><relatesTo>7</relatesTo> (AUTHOR)<i> zeitsan@ms9.hinet.net</i><br /><searchLink fieldCode="AR" term="%22Wang%2C+Yun-Ming%22">Wang, Yun-Ming</searchLink><relatesTo>8</relatesTo> (AUTHOR)<i> jjuang@cgmh.org.tw</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Nanomaterials+%282079-4991%29%22">Nanomaterials (2079-4991)</searchLink>. Nov2021, Vol. 11 Issue 11, p3145-3145. 1p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Iron+oxide+nanoparticles%22">Iron oxide nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Iron+oxides%22">Iron oxides</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetic+resonance+imaging%22">Magnetic resonance imaging</searchLink><br /><searchLink fieldCode="DE" term="%22Contrast+media%22">Contrast media</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetic+nanoparticles%22">Magnetic nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Manganese%22">Manganese</searchLink><br /><searchLink fieldCode="DE" term="%22Islands+of+Langerhans%22">Islands of Langerhans</searchLink><br /><searchLink fieldCode="DE" term="%22Glucagon-like+peptide-1+receptor%22">Glucagon-like peptide-1 receptor</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: To specifically detect and trace transplanted islet β-cells by magnetic resonance imaging (MRI), we conjugated manganese magnetism-engineered iron oxide nanoparticles (MnMEIO NPs) with exendin-4 (Ex4) which specifically binds glucagon-like peptide-1 receptors on the surface of β-cells. The size distribution of MnMEIO and MnMEIO-Ex4 NPs were 67.8 ± 1.3 and 70.2 ± 2.3 nm and zeta potential 33.3 ± 0.5 and 0.6 ± 0.1 mV, respectively. MnMEIO and MnMEIO-Ex4 NPs with iron content ≤ 40 μg/mL did not affect MIN6 β-cell viability and insulin secretion. Positive iron staining was found in MIN6 β-cells loaded with MnMEIO-Ex4 NPs but not in those with MnMEIO NPs. A transmission electron microscope confirmed MnMEIO-Ex4 NPs were distributed in the cytoplasm of MIN6. In vitro MR images revealed a loss of signal intensity in MIN6 β-cells labeled with MnMEIO-Ex4 NPs but not with MnMEIO NPs. After transplantation of islets labeled with MnMEIO-Ex4, the graft under kidney capsule could be visualized on MRI as persistent hypointense areas up to 17 weeks. Moreover, histology of the islet graft showed positive staining for insulin, glucagon and iron. Our results indicate MnMEIO-Ex4 NPs are safe and effective for the detection and long-term monitoring of transplanted β-cells by MRI. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3390/nano11113145 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: 3145 Subjects: – SubjectFull: Iron oxide nanoparticles Type: general – SubjectFull: Iron oxides Type: general – SubjectFull: Magnetic resonance imaging Type: general – SubjectFull: Contrast media Type: general – SubjectFull: Magnetic nanoparticles Type: general – SubjectFull: Manganese Type: general – SubjectFull: Islands of Langerhans Type: general – SubjectFull: Glucagon-like peptide-1 receptor Type: general Titles: – TitleFull: Exendin-4-Conjugated Manganese Magnetism-Engineered Iron Oxide Nanoparticles as a Potential Magnetic Resonance Imaging Contrast Agent for Tracking Transplanted β-Cells. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Juang, Jyuhn-Huarng – PersonEntity: Name: NameFull: Shen, Chia-Rui – PersonEntity: Name: NameFull: Wang, Jiun-Jie – PersonEntity: Name: NameFull: Wu, Shu-Ting – PersonEntity: Name: NameFull: Lin, Sung-Han – PersonEntity: Name: NameFull: Chen, Chen-Yi – PersonEntity: Name: NameFull: Kao, Chen-Wei – PersonEntity: Name: NameFull: Chen, Chen-Ling – PersonEntity: Name: NameFull: Tsai, Zei-Tsan – PersonEntity: Name: NameFull: Wang, Yun-Ming IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 11 Text: Nov2021 Type: published Y: 2021 Identifiers: – Type: issn-print Value: 20794991 Numbering: – Type: volume Value: 11 – Type: issue Value: 11 Titles: – TitleFull: Nanomaterials (2079-4991) Type: main |
| ResultId | 1 |
