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Ultrasensitive polyaniline‐nickel oxide cladding modified with urease immobilized intrinsic optical fiber urea biosensor.

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Title: Ultrasensitive polyaniline‐nickel oxide cladding modified with urease immobilized intrinsic optical fiber urea biosensor.
Authors: Botewad, Sunil N.1 (AUTHOR) sunilb1902@gmail.com, Gaikwad, Dhammajyot K.2 (AUTHOR), Girhe, Nitin B.3 (AUTHOR), Pawar, Pravina P.1 (AUTHOR)
Source: Polymers for Advanced Technologies. Jan2022, Vol. 33 Issue 1, p189-197. 9p.
Subjects: Optical fibers, Polyanilines, Urease, Urea, Biosensors, Optical properties
Abstract: Herein, we report a polyaniline‐nickel oxide (PANI‐NiO) nanocomposite as an efficient immobilization matrix for development the optical fiber urea biosensor. Optical fiber sensing probe was developed by removing some portion of optical fiber at middle and modified with PANI‐NiO matrix. After the modification of cladding removed portion, it was immobilized with enzyme urease via glutaraldehyde as a bi‐functional cross‐linking agent. The physicochemical and optical properties of the PANI‐NiO matrix were explored by X‐ray diffraction, scanning electron microscopy, ultraviolet–visible, and Fourier transform infrared spectroscopic techniques. The characteristic features and performance of the developed sensor were evaluated via recording the output power and modal power distribution by means of a charge‐coupled device camera. The developed urea biosensor exhibits a selective response towards urea concentrations in the linear range 1 nM–100 mM with a lower detection limit of 1 nM. Sensor recorded as a 40 days stability and response time ~1 min. Thus, the obtained experimental results of the developed sensor promote its applicability with practical prospects in diverse field. [ABSTRACT FROM AUTHOR]
Copyright of Polymers for Advanced Technologies is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Ultrasensitive polyaniline‐nickel oxide cladding modified with urease immobilized intrinsic optical fiber urea biosensor.
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  Data: <searchLink fieldCode="AR" term="%22Botewad%2C+Sunil+N%2E%22">Botewad, Sunil N.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> sunilb1902@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Gaikwad%2C+Dhammajyot+K%2E%22">Gaikwad, Dhammajyot K.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Girhe%2C+Nitin+B%2E%22">Girhe, Nitin B.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pawar%2C+Pravina+P%2E%22">Pawar, Pravina P.</searchLink><relatesTo>1</relatesTo> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Polymers+for+Advanced+Technologies%22">Polymers for Advanced Technologies</searchLink>. Jan2022, Vol. 33 Issue 1, p189-197. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Optical+fibers%22">Optical fibers</searchLink><br /><searchLink fieldCode="DE" term="%22Polyanilines%22">Polyanilines</searchLink><br /><searchLink fieldCode="DE" term="%22Urease%22">Urease</searchLink><br /><searchLink fieldCode="DE" term="%22Urea%22">Urea</searchLink><br /><searchLink fieldCode="DE" term="%22Biosensors%22">Biosensors</searchLink><br /><searchLink fieldCode="DE" term="%22Optical+properties%22">Optical properties</searchLink>
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  Label: Abstract
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  Data: Herein, we report a polyaniline‐nickel oxide (PANI‐NiO) nanocomposite as an efficient immobilization matrix for development the optical fiber urea biosensor. Optical fiber sensing probe was developed by removing some portion of optical fiber at middle and modified with PANI‐NiO matrix. After the modification of cladding removed portion, it was immobilized with enzyme urease via glutaraldehyde as a bi‐functional cross‐linking agent. The physicochemical and optical properties of the PANI‐NiO matrix were explored by X‐ray diffraction, scanning electron microscopy, ultraviolet–visible, and Fourier transform infrared spectroscopic techniques. The characteristic features and performance of the developed sensor were evaluated via recording the output power and modal power distribution by means of a charge‐coupled device camera. The developed urea biosensor exhibits a selective response towards urea concentrations in the linear range 1 nM–100 mM with a lower detection limit of 1 nM. Sensor recorded as a 40 days stability and response time ~1 min. Thus, the obtained experimental results of the developed sensor promote its applicability with practical prospects in diverse field. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Polymers for Advanced Technologies is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1002/pat.5504
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      – Code: eng
        Text: English
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        PageCount: 9
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      – SubjectFull: Optical fibers
        Type: general
      – SubjectFull: Polyanilines
        Type: general
      – SubjectFull: Urease
        Type: general
      – SubjectFull: Urea
        Type: general
      – SubjectFull: Biosensors
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      – SubjectFull: Optical properties
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      – TitleFull: Ultrasensitive polyaniline‐nickel oxide cladding modified with urease immobilized intrinsic optical fiber urea biosensor.
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            NameFull: Gaikwad, Dhammajyot K.
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            NameFull: Girhe, Nitin B.
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              Text: Jan2022
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              Y: 2022
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