A fully integrated biomimetic microfluidic device for evaluation of sperm response to thermotaxis and chemotaxis.
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| Title: | A fully integrated biomimetic microfluidic device for evaluation of sperm response to thermotaxis and chemotaxis. |
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| Authors: | Yan, Yimo1,2 (AUTHOR), Zhang, Boxuan1,2 (AUTHOR), Fu, Qiqi1 (AUTHOR), Wu, Jian2 (AUTHOR), Liu, Ran1 (AUTHOR) liuran@tsinghua.edu.cn |
| Source: | Lab on a Chip. 1/14/2021, Vol. 21 Issue 2, p310-318. 9p. |
| Subjects: | Chemotaxis, Human reproductive technology, Genitalia, Reproductive technology, Microfluidic devices, Spermatozoa, Medical screening |
| Abstract: | In recent decades, humans have faced greater challenges in reproduction. Assisted reproductive technology is the most prominent approach for addressing this problem. Current clinical screening methods simply consider the motility or morphology of the sperm. However, as the spermatozoa need to navigate over a long distance in the female reproductive tract and survive the natural screening processes therein, these methods are imperfect. Many approaches have been undertaken to study the chemotaxis and thermotaxis navigation behavior of spermatozoa, but few of these have involved integrated screening that considers motility, chemotaxis, and thermotaxis based on the biological environment of the human body. Current routine sperm evaluation techniques are inadequate and fail to simultaneously provide conclusive evidence for the thermotactic and chemotactic characteristics of sperm. Thus, such screening of functional spermatozoa will be an advancement in assisted reproduction. In this study, we developed a fully integrated biomimetic microfluidic system for screening sperm for their characteristics when exposed to temperature and chemical gradients. Based on our results, we showed that spermatozoa were attracted by temperature and chemical gradients in the physiological range. Moreover, we ascertained a suitable temperature gradient range for thermotaxis and statistically proved that the thermotactic and chemotactic responses are not linked. Here, we report the first quantitative study of functional sperm during thermotaxis and chemotaxis, and our analysis of the difference in motility caused by different conditions. More broadly, we foresee the clinical application of these biologically motivated parameters and characteristics in assisted reproduction in humans. [ABSTRACT FROM AUTHOR] |
| Copyright of Lab on a Chip is the property of Royal Society of Chemistry and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 154435723 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: A fully integrated biomimetic microfluidic device for evaluation of sperm response to thermotaxis and chemotaxis. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Yan%2C+Yimo%22">Yan, Yimo</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Boxuan%22">Zhang, Boxuan</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Fu%2C+Qiqi%22">Fu, Qiqi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wu%2C+Jian%22">Wu, Jian</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Ran%22">Liu, Ran</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> liuran@tsinghua.edu.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Lab+on+a+Chip%22">Lab on a Chip</searchLink>. 1/14/2021, Vol. 21 Issue 2, p310-318. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Chemotaxis%22">Chemotaxis</searchLink><br /><searchLink fieldCode="DE" term="%22Human+reproductive+technology%22">Human reproductive technology</searchLink><br /><searchLink fieldCode="DE" term="%22Genitalia%22">Genitalia</searchLink><br /><searchLink fieldCode="DE" term="%22Reproductive+technology%22">Reproductive technology</searchLink><br /><searchLink fieldCode="DE" term="%22Microfluidic+devices%22">Microfluidic devices</searchLink><br /><searchLink fieldCode="DE" term="%22Spermatozoa%22">Spermatozoa</searchLink><br /><searchLink fieldCode="DE" term="%22Medical+screening%22">Medical screening</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: In recent decades, humans have faced greater challenges in reproduction. Assisted reproductive technology is the most prominent approach for addressing this problem. Current clinical screening methods simply consider the motility or morphology of the sperm. However, as the spermatozoa need to navigate over a long distance in the female reproductive tract and survive the natural screening processes therein, these methods are imperfect. Many approaches have been undertaken to study the chemotaxis and thermotaxis navigation behavior of spermatozoa, but few of these have involved integrated screening that considers motility, chemotaxis, and thermotaxis based on the biological environment of the human body. Current routine sperm evaluation techniques are inadequate and fail to simultaneously provide conclusive evidence for the thermotactic and chemotactic characteristics of sperm. Thus, such screening of functional spermatozoa will be an advancement in assisted reproduction. In this study, we developed a fully integrated biomimetic microfluidic system for screening sperm for their characteristics when exposed to temperature and chemical gradients. Based on our results, we showed that spermatozoa were attracted by temperature and chemical gradients in the physiological range. Moreover, we ascertained a suitable temperature gradient range for thermotaxis and statistically proved that the thermotactic and chemotactic responses are not linked. Here, we report the first quantitative study of functional sperm during thermotaxis and chemotaxis, and our analysis of the difference in motility caused by different conditions. More broadly, we foresee the clinical application of these biologically motivated parameters and characteristics in assisted reproduction in humans. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Lab on a Chip is the property of Royal Society of Chemistry and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1039/d0lc00845a Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 310 Subjects: – SubjectFull: Chemotaxis Type: general – SubjectFull: Human reproductive technology Type: general – SubjectFull: Genitalia Type: general – SubjectFull: Reproductive technology Type: general – SubjectFull: Microfluidic devices Type: general – SubjectFull: Spermatozoa Type: general – SubjectFull: Medical screening Type: general Titles: – TitleFull: A fully integrated biomimetic microfluidic device for evaluation of sperm response to thermotaxis and chemotaxis. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Yan, Yimo – PersonEntity: Name: NameFull: Zhang, Boxuan – PersonEntity: Name: NameFull: Fu, Qiqi – PersonEntity: Name: NameFull: Wu, Jian – PersonEntity: Name: NameFull: Liu, Ran IsPartOfRelationships: – BibEntity: Dates: – D: 14 M: 01 Text: 1/14/2021 Type: published Y: 2021 Identifiers: – Type: issn-print Value: 14730197 Numbering: – Type: volume Value: 21 – Type: issue Value: 2 Titles: – TitleFull: Lab on a Chip Type: main |
| ResultId | 1 |
