View in EDS

A general approach to explore prokaryotic protein glycosylation reveals the unique surface layer modulation of an anammox bacterium.

Saved in:
Bibliographic Details
Title: A general approach to explore prokaryotic protein glycosylation reveals the unique surface layer modulation of an anammox bacterium.
Authors: Pabst, Martin1 (AUTHOR) m.pabst@tudelft.nl, Grouzdev, Denis S.2 (AUTHOR), Lawson, Christopher E.3 (AUTHOR), Kleikamp, Hugo B. C.1 (AUTHOR), de Ram, Carol1 (AUTHOR), Louwen, Rogier4 (AUTHOR), Lin, Yue Mei1 (AUTHOR), Lücker, Sebastian5 (AUTHOR), van Loosdrecht, Mark C. M.1 (AUTHOR), Laureni, Michele1 (AUTHOR)
Source: ISME Journal: Multidisciplinary Journal of Microbial Ecology. Feb2022, Vol. 16 Issue 2, p346-357. 12p.
Abstract: The enormous chemical diversity and strain variability of prokaryotic protein glycosylation makes their large-scale exploration exceptionally challenging. Therefore, despite the universal relevance of protein glycosylation across all domains of life, the understanding of their biological significance and the evolutionary forces shaping oligosaccharide structures remains highly limited. Here, we report on a newly established mass binning glycoproteomics approach that establishes the chemical identity of the carbohydrate components and performs untargeted exploration of prokaryotic oligosaccharides from large-scale proteomics data directly. We demonstrate our approach by exploring an enrichment culture of the globally relevant anaerobic ammonium-oxidizing bacterium Ca. Kuenenia stuttgartiensis. By doing so we resolve a remarkable array of oligosaccharides, which are produced by two seemingly unrelated biosynthetic routes, and which modify the same surface-layer protein simultaneously. More intriguingly, the investigated strain also accomplished modulation of highly specialized sugars, supposedly in response to its energy metabolism—the anaerobic oxidation of ammonium—which depends on the acquisition of substrates of opposite charges. Ultimately, we provide a systematic approach for the compositional exploration of prokaryotic protein glycosylation, and reveal a remarkable example for the evolution of complex oligosaccharides in bacteria. [ABSTRACT FROM AUTHOR]
Copyright of ISME Journal: Multidisciplinary Journal of Microbial Ecology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 154791875
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: A general approach to explore prokaryotic protein glycosylation reveals the unique surface layer modulation of an anammox bacterium.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Pabst%2C+Martin%22">Pabst, Martin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> m.pabst@tudelft.nl</i><br /><searchLink fieldCode="AR" term="%22Grouzdev%2C+Denis+S%2E%22">Grouzdev, Denis S.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lawson%2C+Christopher+E%2E%22">Lawson, Christopher E.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kleikamp%2C+Hugo+B%2E+C%2E%22">Kleikamp, Hugo B. C.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22de+Ram%2C+Carol%22">de Ram, Carol</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Louwen%2C+Rogier%22">Louwen, Rogier</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lin%2C+Yue+Mei%22">Lin, Yue Mei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lücker%2C+Sebastian%22">Lücker, Sebastian</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22van+Loosdrecht%2C+Mark+C%2E+M%2E%22">van Loosdrecht, Mark C. M.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Laureni%2C+Michele%22">Laureni, Michele</searchLink><relatesTo>1</relatesTo> (AUTHOR)
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22ISME+Journal%3A+Multidisciplinary+Journal+of+Microbial+Ecology%22">ISME Journal: Multidisciplinary Journal of Microbial Ecology</searchLink>. Feb2022, Vol. 16 Issue 2, p346-357. 12p.
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The enormous chemical diversity and strain variability of prokaryotic protein glycosylation makes their large-scale exploration exceptionally challenging. Therefore, despite the universal relevance of protein glycosylation across all domains of life, the understanding of their biological significance and the evolutionary forces shaping oligosaccharide structures remains highly limited. Here, we report on a newly established mass binning glycoproteomics approach that establishes the chemical identity of the carbohydrate components and performs untargeted exploration of prokaryotic oligosaccharides from large-scale proteomics data directly. We demonstrate our approach by exploring an enrichment culture of the globally relevant anaerobic ammonium-oxidizing bacterium Ca. Kuenenia stuttgartiensis. By doing so we resolve a remarkable array of oligosaccharides, which are produced by two seemingly unrelated biosynthetic routes, and which modify the same surface-layer protein simultaneously. More intriguingly, the investigated strain also accomplished modulation of highly specialized sugars, supposedly in response to its energy metabolism—the anaerobic oxidation of ammonium—which depends on the acquisition of substrates of opposite charges. Ultimately, we provide a systematic approach for the compositional exploration of prokaryotic protein glycosylation, and reveal a remarkable example for the evolution of complex oligosaccharides in bacteria. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of ISME Journal: Multidisciplinary Journal of Microbial Ecology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=154791875
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1038/s41396-021-01073-y
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 12
        StartPage: 346
    Titles:
      – TitleFull: A general approach to explore prokaryotic protein glycosylation reveals the unique surface layer modulation of an anammox bacterium.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Pabst, Martin
      – PersonEntity:
          Name:
            NameFull: Grouzdev, Denis S.
      – PersonEntity:
          Name:
            NameFull: Lawson, Christopher E.
      – PersonEntity:
          Name:
            NameFull: Kleikamp, Hugo B. C.
      – PersonEntity:
          Name:
            NameFull: de Ram, Carol
      – PersonEntity:
          Name:
            NameFull: Louwen, Rogier
      – PersonEntity:
          Name:
            NameFull: Lin, Yue Mei
      – PersonEntity:
          Name:
            NameFull: Lücker, Sebastian
      – PersonEntity:
          Name:
            NameFull: van Loosdrecht, Mark C. M.
      – PersonEntity:
          Name:
            NameFull: Laureni, Michele
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 02
              Text: Feb2022
              Type: published
              Y: 2022
          Identifiers:
            – Type: issn-print
              Value: 17517362
          Numbering:
            – Type: volume
              Value: 16
            – Type: issue
              Value: 2
          Titles:
            – TitleFull: ISME Journal: Multidisciplinary Journal of Microbial Ecology
              Type: main
ResultId 1