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Near-infrared 940-nm diode laser photobiomodulation of inflamed periodontal ligament stem cells.

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Title: Near-infrared 940-nm diode laser photobiomodulation of inflamed periodontal ligament stem cells.
Authors: Gholami, Leila1 (AUTHOR), Hendi, Seyedeh Sareh2 (AUTHOR) hendispx@yahoo.com, Saidijam, Massoud3 (AUTHOR), Mahmoudi, Roghayeh3 (AUTHOR), Tarzemany, Rana4 (AUTHOR), Arkian, Aliasghar5 (AUTHOR), Afshar, Saeid3 (AUTHOR) safshar.h@gmail.com, Fekrazad, Reza6,7 (AUTHOR)
Source: Lasers in Medical Science. Feb2022, Vol. 37 Issue 1, p449-459. 11p.
Subjects: Periodontal ligament, Stem cells, Photobiomodulation therapy, Semiconductor lasers, Alkaline phosphatase, Photoreceptors, Cell survival
Abstract: Photobiomodulation (PBM) is an acceptable method of stimulating stem cells through its non-invasive absorption by the cell photoreceptors and the induction of cellular response. The current research was aimed at evaluating the effect of near-infrared PBM on proliferation and osteogenic differentiation in inflamed periodontal ligament stem cells (I-PDLSCs). I-PDLSCs were isolated and characterized. Third passage cells were irradiated with 940-nm laser at an output power of 100 mW in a continuous wave. A fluence of 4 J/cm2 in three sessions at 48-h intervals was applied and compared with non-irradiated controls. Cell viability and proliferation were evaluated by MTT assay. Alkaline phosphatase activity, quantitative Alizarin red staining test, and q-RT-PCR were used to evaluate the osteogenic properties of the I-PDLSCs in four groups of (a) osteogenic differentiation medium + laser (ODM + L), (b) osteogenic differentiation medium without laser (ODM), (c) non-osteogenic differentiation medium + laser (L), and (d) non-osteogenic differentiation medium (control). There was a non-significant increase in the viability of cells at 48- and 72-h post last laser irradiation. Alizarin red staining revealed no significant stimulatory effect of PBM at 14 and 21 days. However, alkaline phosphatase activity was significantly higher in the L + ODM group. Expression of osteogenic-related genes had a statistically significant increase at 21-day post irradiation. The irradiation used in the present study showed no significant increase in the proliferation of I-PDLSCs by PBM. However, expression levels of osteogenic-related genes and alkaline phosphatase activity were significantly increased in irradiated groups. [ABSTRACT FROM AUTHOR]
Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Near-infrared 940-nm diode laser photobiomodulation of inflamed periodontal ligament stem cells.
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  Data: <searchLink fieldCode="AR" term="%22Gholami%2C+Leila%22">Gholami, Leila</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hendi%2C+Seyedeh+Sareh%22">Hendi, Seyedeh Sareh</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> hendispx@yahoo.com</i><br /><searchLink fieldCode="AR" term="%22Saidijam%2C+Massoud%22">Saidijam, Massoud</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mahmoudi%2C+Roghayeh%22">Mahmoudi, Roghayeh</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tarzemany%2C+Rana%22">Tarzemany, Rana</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Arkian%2C+Aliasghar%22">Arkian, Aliasghar</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Afshar%2C+Saeid%22">Afshar, Saeid</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> safshar.h@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Fekrazad%2C+Reza%22">Fekrazad, Reza</searchLink><relatesTo>6,7</relatesTo> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Lasers+in+Medical+Science%22">Lasers in Medical Science</searchLink>. Feb2022, Vol. 37 Issue 1, p449-459. 11p.
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  Data: <searchLink fieldCode="DE" term="%22Periodontal+ligament%22">Periodontal ligament</searchLink><br /><searchLink fieldCode="DE" term="%22Stem+cells%22">Stem cells</searchLink><br /><searchLink fieldCode="DE" term="%22Photobiomodulation+therapy%22">Photobiomodulation therapy</searchLink><br /><searchLink fieldCode="DE" term="%22Semiconductor+lasers%22">Semiconductor lasers</searchLink><br /><searchLink fieldCode="DE" term="%22Alkaline+phosphatase%22">Alkaline phosphatase</searchLink><br /><searchLink fieldCode="DE" term="%22Photoreceptors%22">Photoreceptors</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+survival%22">Cell survival</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Photobiomodulation (PBM) is an acceptable method of stimulating stem cells through its non-invasive absorption by the cell photoreceptors and the induction of cellular response. The current research was aimed at evaluating the effect of near-infrared PBM on proliferation and osteogenic differentiation in inflamed periodontal ligament stem cells (I-PDLSCs). I-PDLSCs were isolated and characterized. Third passage cells were irradiated with 940-nm laser at an output power of 100 mW in a continuous wave. A fluence of 4 J/cm2 in three sessions at 48-h intervals was applied and compared with non-irradiated controls. Cell viability and proliferation were evaluated by MTT assay. Alkaline phosphatase activity, quantitative Alizarin red staining test, and q-RT-PCR were used to evaluate the osteogenic properties of the I-PDLSCs in four groups of (a) osteogenic differentiation medium + laser (ODM + L), (b) osteogenic differentiation medium without laser (ODM), (c) non-osteogenic differentiation medium + laser (L), and (d) non-osteogenic differentiation medium (control). There was a non-significant increase in the viability of cells at 48- and 72-h post last laser irradiation. Alizarin red staining revealed no significant stimulatory effect of PBM at 14 and 21 days. However, alkaline phosphatase activity was significantly higher in the L + ODM group. Expression of osteogenic-related genes had a statistically significant increase at 21-day post irradiation. The irradiation used in the present study showed no significant increase in the proliferation of I-PDLSCs by PBM. However, expression levels of osteogenic-related genes and alkaline phosphatase activity were significantly increased in irradiated groups. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1007/s10103-021-03282-5
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        Text: English
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        PageCount: 11
        StartPage: 449
    Subjects:
      – SubjectFull: Periodontal ligament
        Type: general
      – SubjectFull: Stem cells
        Type: general
      – SubjectFull: Photobiomodulation therapy
        Type: general
      – SubjectFull: Semiconductor lasers
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      – SubjectFull: Alkaline phosphatase
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      – SubjectFull: Photoreceptors
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      – SubjectFull: Cell survival
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    Titles:
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              Text: Feb2022
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              Y: 2022
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