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Enhancing the performance of sterile filtration for viral vaccines and model nanoparticles using an appropriate prefilter.

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Title: Enhancing the performance of sterile filtration for viral vaccines and model nanoparticles using an appropriate prefilter.
Authors: Taylor, Neil1 (AUTHOR), Ma, Wanli (Justin)2 (AUTHOR), Kristopeit, Adam2 (AUTHOR), Wang, Sheng-Ching2 (AUTHOR), Zydney, Andrew L.1 (AUTHOR) zydney@engr.psu.edu
Source: Journal of Membrane Science. Apr2022, Vol. 647, pN.PAG-N.PAG. 1p.
Subjects: Viral vaccines, Porosity, Viral transmission, Nanoparticles
Abstract: Prefilters are widely used to enhance the performance of normal flow filtration steps in bioprocessing; however little is known about the potential benefits of prefiltration in the sterile filtration of viral vaccines and other large particle biotherapeutics. Sterile filtration experiments were performed with a live-attenuated viral vaccine and model nanoparticle suspension, both of which have a size distribution of 100 – 400 nm. The results demonstrated that the outstanding performance of the Sartobran P sterile filter was directly due to the effective prefiltration provided by the 0.45 μm prefilter in this dual layer filter. The 0.45 μm prefilter dramatically improved particle transmission and capacity for other 0.2 μm sterile filters. The key foulants were removed primarily on the basis of size, which was confirmed using a nanoparticle tracking analyzer. Larger pore size prefilters were found to be much less effective in protecting the 0.2 μm sterile filter. The support structure of asymmetric sterile filters also provided significant prefiltration; however, some of these asymmetric membranes had size-selective pores that were too small to provide significant transmission of the viral particles. These results provided important insights into the role of the prefilter layer and membrane pore structure in enhancing the performance of the sterile filtration step for the processing of viruses and other large particle biotherapeutics. [Display omitted] • Sterile filtration performed with Live Attenuated Viral vaccine (LAV). • Role of prefilter in Sartobran P dual-layer filter quantified. • Prefilter improves capacity and transmission by removing large particles. • Support structure of asymmetric membrane also provides effective prefiltration. • Combination of prefilter and sterile filter pore size determines performance. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Membrane Science is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 155257932
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: Enhancing the performance of sterile filtration for viral vaccines and model nanoparticles using an appropriate prefilter.
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  Data: <searchLink fieldCode="AR" term="%22Taylor%2C+Neil%22">Taylor, Neil</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ma%2C+Wanli+%28Justin%29%22">Ma, Wanli (Justin)</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kristopeit%2C+Adam%22">Kristopeit, Adam</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Sheng-Ching%22">Wang, Sheng-Ching</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zydney%2C+Andrew+L%2E%22">Zydney, Andrew L.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> zydney@engr.psu.edu</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Membrane+Science%22">Journal of Membrane Science</searchLink>. Apr2022, Vol. 647, pN.PAG-N.PAG. 1p.
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  Data: <searchLink fieldCode="DE" term="%22Viral+vaccines%22">Viral vaccines</searchLink><br /><searchLink fieldCode="DE" term="%22Porosity%22">Porosity</searchLink><br /><searchLink fieldCode="DE" term="%22Viral+transmission%22">Viral transmission</searchLink><br /><searchLink fieldCode="DE" term="%22Nanoparticles%22">Nanoparticles</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Prefilters are widely used to enhance the performance of normal flow filtration steps in bioprocessing; however little is known about the potential benefits of prefiltration in the sterile filtration of viral vaccines and other large particle biotherapeutics. Sterile filtration experiments were performed with a live-attenuated viral vaccine and model nanoparticle suspension, both of which have a size distribution of 100 – 400 nm. The results demonstrated that the outstanding performance of the Sartobran P sterile filter was directly due to the effective prefiltration provided by the 0.45 μm prefilter in this dual layer filter. The 0.45 μm prefilter dramatically improved particle transmission and capacity for other 0.2 μm sterile filters. The key foulants were removed primarily on the basis of size, which was confirmed using a nanoparticle tracking analyzer. Larger pore size prefilters were found to be much less effective in protecting the 0.2 μm sterile filter. The support structure of asymmetric sterile filters also provided significant prefiltration; however, some of these asymmetric membranes had size-selective pores that were too small to provide significant transmission of the viral particles. These results provided important insights into the role of the prefilter layer and membrane pore structure in enhancing the performance of the sterile filtration step for the processing of viruses and other large particle biotherapeutics. [Display omitted] • Sterile filtration performed with Live Attenuated Viral vaccine (LAV). • Role of prefilter in Sartobran P dual-layer filter quantified. • Prefilter improves capacity and transmission by removing large particles. • Support structure of asymmetric membrane also provides effective prefiltration. • Combination of prefilter and sterile filter pore size determines performance. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Membrane Science is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.memsci.2022.120264
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      – Code: eng
        Text: English
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        PageCount: 1
        StartPage: N.PAG
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      – SubjectFull: Viral vaccines
        Type: general
      – SubjectFull: Porosity
        Type: general
      – SubjectFull: Viral transmission
        Type: general
      – SubjectFull: Nanoparticles
        Type: general
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      – TitleFull: Enhancing the performance of sterile filtration for viral vaccines and model nanoparticles using an appropriate prefilter.
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            NameFull: Taylor, Neil
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            NameFull: Ma, Wanli (Justin)
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            NameFull: Kristopeit, Adam
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            NameFull: Wang, Sheng-Ching
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            NameFull: Zydney, Andrew L.
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            – D: 05
              M: 04
              Text: Apr2022
              Type: published
              Y: 2022
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              Value: 647
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