Magnetic Resonance Imaging of Transplanted Porcine Neonatal Pancreatic Cell Clusters Labeled with Exendin-4-Conjugated Manganese Magnetism-Engineered Iron Oxide Nanoparticles.
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| Title: | Magnetic Resonance Imaging of Transplanted Porcine Neonatal Pancreatic Cell Clusters Labeled with Exendin-4-Conjugated Manganese Magnetism-Engineered Iron Oxide Nanoparticles. |
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| Authors: | Juang, Jyuhn-Huarng1,2 (AUTHOR) je3474@gmail.com, Wang, Jiun-Jie3,4 (AUTHOR) jiunjie.wang@gmail.com, Shen, Chia-Rui5,6 (AUTHOR) crshen@mail.cgu.edu.tw, Lin, Sung-Han3 (AUTHOR) image.lin@gmail.com, Chen, Chen-Yi1 (AUTHOR) lian8807111@gmail.com, Kao, Chen-Wei1 (AUTHOR) jenny74513@gmail.com, Chen, Chen-Ling1 (AUTHOR), Wu, Shu-Ting5 (AUTHOR) proteinwhite@livemail.tw, Tsai, Zei-Tsan7 (AUTHOR) zeitsan@ms9.hinet.net, Wang, Yun-Ming8 (AUTHOR) jjuang@cgmh.org.tw |
| Source: | Nanomaterials (2079-4991). Apr2022, Vol. 12 Issue 7, p1222-1222. 13p. |
| Subjects: | Iron oxide nanoparticles, Magnetic resonance imaging, Contrast media, Magnetic nanoparticles, Prussian blue, Iron oxides, Gadolinium |
| Abstract: | Recently, we have shown that manganese magnetism-engineered iron oxide nanoparticles (MnMEIO NPs) conjugated with exendin-4 (Ex4) act as a contrast agent that directly trace implanted mouse islet β-cells by magnetic resonance imaging (MRI). Here we further advanced this technology to track implanted porcine neonatal pancreatic cell clusters (NPCCs) containing ducts, endocrine, and exocrine cells. NPCCs from one-day-old neonatal pigs were isolated, cultured for three days, and then incubated overnight with MnMEIO-Ex4 NPs. Binding of NPCCs and MnMEIO-Ex4 NPs was confirmed with Prussian blue staining in vitro prior to the transplantation of 2000 MnMEIO-Ex4 NP-labeled NPCCs beneath the left renal capsule of six nondiabetic nude mice. The 7.0 T MRI on recipients revealed persistent hypointense areas at implantation sites for up to 54 days. The MR signal intensity of the graft on left kidney reduced 62–88% compared to the mirror areas on the contralateral kidney. Histological studies showed colocalization of insulin/iron and SOX9/iron staining in NPCC grafts, indicating that MnMEIO-Ex4 NPs were taken up by mature β-cells and pancreatic progenitors. We conclude that MnMEIO-Ex4 NPs are excellent contrast agents for detecting and long-term monitoring implanted NPCCs by MRI. [ABSTRACT FROM AUTHOR] |
| Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: egs DbLabel: Engineering Source An: 156325289 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Magnetic Resonance Imaging of Transplanted Porcine Neonatal Pancreatic Cell Clusters Labeled with Exendin-4-Conjugated Manganese Magnetism-Engineered Iron Oxide Nanoparticles. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Juang%2C+Jyuhn-Huarng%22">Juang, Jyuhn-Huarng</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> je3474@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Wang%2C+Jiun-Jie%22">Wang, Jiun-Jie</searchLink><relatesTo>3,4</relatesTo> (AUTHOR)<i> jiunjie.wang@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Shen%2C+Chia-Rui%22">Shen, Chia-Rui</searchLink><relatesTo>5,6</relatesTo> (AUTHOR)<i> crshen@mail.cgu.edu.tw</i><br /><searchLink fieldCode="AR" term="%22Lin%2C+Sung-Han%22">Lin, Sung-Han</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> image.lin@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Chen%2C+Chen-Yi%22">Chen, Chen-Yi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> lian8807111@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Kao%2C+Chen-Wei%22">Kao, Chen-Wei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> jenny74513@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Chen%2C+Chen-Ling%22">Chen, Chen-Ling</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wu%2C+Shu-Ting%22">Wu, Shu-Ting</searchLink><relatesTo>5</relatesTo> (AUTHOR)<i> proteinwhite@livemail.tw</i><br /><searchLink fieldCode="AR" term="%22Tsai%2C+Zei-Tsan%22">Tsai, Zei-Tsan</searchLink><relatesTo>7</relatesTo> (AUTHOR)<i> zeitsan@ms9.hinet.net</i><br /><searchLink fieldCode="AR" term="%22Wang%2C+Yun-Ming%22">Wang, Yun-Ming</searchLink><relatesTo>8</relatesTo> (AUTHOR)<i> jjuang@cgmh.org.tw</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Nanomaterials+%282079-4991%29%22">Nanomaterials (2079-4991)</searchLink>. Apr2022, Vol. 12 Issue 7, p1222-1222. 13p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Iron+oxide+nanoparticles%22">Iron oxide nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetic+resonance+imaging%22">Magnetic resonance imaging</searchLink><br /><searchLink fieldCode="DE" term="%22Contrast+media%22">Contrast media</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetic+nanoparticles%22">Magnetic nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Prussian+blue%22">Prussian blue</searchLink><br /><searchLink fieldCode="DE" term="%22Iron+oxides%22">Iron oxides</searchLink><br /><searchLink fieldCode="DE" term="%22Gadolinium%22">Gadolinium</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Recently, we have shown that manganese magnetism-engineered iron oxide nanoparticles (MnMEIO NPs) conjugated with exendin-4 (Ex4) act as a contrast agent that directly trace implanted mouse islet β-cells by magnetic resonance imaging (MRI). Here we further advanced this technology to track implanted porcine neonatal pancreatic cell clusters (NPCCs) containing ducts, endocrine, and exocrine cells. NPCCs from one-day-old neonatal pigs were isolated, cultured for three days, and then incubated overnight with MnMEIO-Ex4 NPs. Binding of NPCCs and MnMEIO-Ex4 NPs was confirmed with Prussian blue staining in vitro prior to the transplantation of 2000 MnMEIO-Ex4 NP-labeled NPCCs beneath the left renal capsule of six nondiabetic nude mice. The 7.0 T MRI on recipients revealed persistent hypointense areas at implantation sites for up to 54 days. The MR signal intensity of the graft on left kidney reduced 62–88% compared to the mirror areas on the contralateral kidney. Histological studies showed colocalization of insulin/iron and SOX9/iron staining in NPCC grafts, indicating that MnMEIO-Ex4 NPs were taken up by mature β-cells and pancreatic progenitors. We conclude that MnMEIO-Ex4 NPs are excellent contrast agents for detecting and long-term monitoring implanted NPCCs by MRI. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3390/nano12071222 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 13 StartPage: 1222 Subjects: – SubjectFull: Iron oxide nanoparticles Type: general – SubjectFull: Magnetic resonance imaging Type: general – SubjectFull: Contrast media Type: general – SubjectFull: Magnetic nanoparticles Type: general – SubjectFull: Prussian blue Type: general – SubjectFull: Iron oxides Type: general – SubjectFull: Gadolinium Type: general Titles: – TitleFull: Magnetic Resonance Imaging of Transplanted Porcine Neonatal Pancreatic Cell Clusters Labeled with Exendin-4-Conjugated Manganese Magnetism-Engineered Iron Oxide Nanoparticles. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Juang, Jyuhn-Huarng – PersonEntity: Name: NameFull: Wang, Jiun-Jie – PersonEntity: Name: NameFull: Shen, Chia-Rui – PersonEntity: Name: NameFull: Lin, Sung-Han – PersonEntity: Name: NameFull: Chen, Chen-Yi – PersonEntity: Name: NameFull: Kao, Chen-Wei – PersonEntity: Name: NameFull: Chen, Chen-Ling – PersonEntity: Name: NameFull: Wu, Shu-Ting – PersonEntity: Name: NameFull: Tsai, Zei-Tsan – PersonEntity: Name: NameFull: Wang, Yun-Ming IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2022 Type: published Y: 2022 Identifiers: – Type: issn-print Value: 20794991 Numbering: – Type: volume Value: 12 – Type: issue Value: 7 Titles: – TitleFull: Nanomaterials (2079-4991) Type: main |
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