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The pmrF polymyxin-resistance operon of Yersinia pseudotuberculosis is upregulated by the Pho-P-PhoQ two-component system but not by PmrA-PmrB, and is not required for virulence.

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Title: The pmrF polymyxin-resistance operon of Yersinia pseudotuberculosis is upregulated by the Pho-P-PhoQ two-component system but not by PmrA-PmrB, and is not required for virulence.
Authors: Marceau, M.1 michael.marceau@ibl.fr, Sebbane, F.1, Ewann, F.2, Collyn, F.1, Lindner, B.3, Campos, M. A.4, Bengoechea, J.-A.4, Simonet, M.1
Source: Microbiology (13500872). Dec2004, Vol. 150 Issue 12, p3947-3957. 11p. 1 Diagram, 3 Charts, 3 Graphs.
Subjects: Yersinia pseudotuberculosis, Operons, Pseudotuberculosis, Chromosomes, Polymyxin, Antibacterial agents, Salmonella enteritidis, Mass spectrometry, Microbiology
Abstract: The Yersinia pseudotuberculosis chromosome contains a seven-gene polycistronic unit (the pmrF operon) whose products share extensive homologies with their pmrF counterparts in Salmonella enterica serovar Typhimurium (S. typhimurium), another Gram-negative bacterial enteropathogen. This gene cluster is essential for addition of 4-aminoarabinose to the lipid moiety of LPS, as demonstrated by MALDI-TOF mass spectrometry of lipid A from both wild-type and pmrF-mutated strains. As in S. typhimurium. 4-aminoarabinose substitution of lipid A contributes to In vitro resistance of Y. pseudotuberculosis to the antimicrobial peptide polymyxin B. Whereas pmrF-expression in S. typhimunum is mediated by both the PhoP—PhoQ and PmrA—PmrB two-component regulatory systems, it appears to be PmrA—PmrB-independent in Y. pseudotuberculosis, with the response regulator PhoP interacting directly with the pmrF operon promoter region. This result reveals that the ubiquitous PmrA—PmrB regulatory system controls different regulons in distinct bacterial species In addition, pmrF inactivation in Y: pseudotuberculosis has no effect on bacterial virulence in the mouse again in contrast to the situation in S typhimurium. The marked differences in pmrF operon regulation in these two phylogenetically close bacterial species may be related to their dissimilar lifestyles. [ABSTRACT FROM AUTHOR]
Copyright of Microbiology (13500872) is the property of Microbiology Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: The pmrF polymyxin-resistance operon of Yersinia pseudotuberculosis is upregulated by the Pho-P-PhoQ two-component system but not by PmrA-PmrB, and is not required for virulence.
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  Data: <searchLink fieldCode="AR" term="%22Marceau%2C+M%2E%22">Marceau, M.</searchLink><relatesTo>1</relatesTo><i> michael.marceau@ibl.fr</i><br /><searchLink fieldCode="AR" term="%22Sebbane%2C+F%2E%22">Sebbane, F.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ewann%2C+F%2E%22">Ewann, F.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Collyn%2C+F%2E%22">Collyn, F.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lindner%2C+B%2E%22">Lindner, B.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Campos%2C+M%2E+A%2E%22">Campos, M. A.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Bengoechea%2C+J%2E-A%2E%22">Bengoechea, J.-A.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Simonet%2C+M%2E%22">Simonet, M.</searchLink><relatesTo>1</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Microbiology+%2813500872%29%22">Microbiology (13500872)</searchLink>. Dec2004, Vol. 150 Issue 12, p3947-3957. 11p. 1 Diagram, 3 Charts, 3 Graphs.
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  Data: <searchLink fieldCode="DE" term="%22Yersinia+pseudotuberculosis%22">Yersinia pseudotuberculosis</searchLink><br /><searchLink fieldCode="DE" term="%22Operons%22">Operons</searchLink><br /><searchLink fieldCode="DE" term="%22Pseudotuberculosis%22">Pseudotuberculosis</searchLink><br /><searchLink fieldCode="DE" term="%22Chromosomes%22">Chromosomes</searchLink><br /><searchLink fieldCode="DE" term="%22Polymyxin%22">Polymyxin</searchLink><br /><searchLink fieldCode="DE" term="%22Antibacterial+agents%22">Antibacterial agents</searchLink><br /><searchLink fieldCode="DE" term="%22Salmonella+enteritidis%22">Salmonella enteritidis</searchLink><br /><searchLink fieldCode="DE" term="%22Mass+spectrometry%22">Mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Microbiology%22">Microbiology</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: The Yersinia pseudotuberculosis chromosome contains a seven-gene polycistronic unit (the pmrF operon) whose products share extensive homologies with their pmrF counterparts in Salmonella enterica serovar Typhimurium (S. typhimurium), another Gram-negative bacterial enteropathogen. This gene cluster is essential for addition of 4-aminoarabinose to the lipid moiety of LPS, as demonstrated by MALDI-TOF mass spectrometry of lipid A from both wild-type and pmrF-mutated strains. As in S. typhimurium. 4-aminoarabinose substitution of lipid A contributes to In vitro resistance of Y. pseudotuberculosis to the antimicrobial peptide polymyxin B. Whereas pmrF-expression in S. typhimunum is mediated by both the PhoP—PhoQ and PmrA—PmrB two-component regulatory systems, it appears to be PmrA—PmrB-independent in Y. pseudotuberculosis, with the response regulator PhoP interacting directly with the pmrF operon promoter region. This result reveals that the ubiquitous PmrA—PmrB regulatory system controls different regulons in distinct bacterial species In addition, pmrF inactivation in Y: pseudotuberculosis has no effect on bacterial virulence in the mouse again in contrast to the situation in S typhimurium. The marked differences in pmrF operon regulation in these two phylogenetically close bacterial species may be related to their dissimilar lifestyles. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Microbiology (13500872) is the property of Microbiology Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1099/mic.0.27426-0
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        Text: English
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        PageCount: 11
        StartPage: 3947
    Subjects:
      – SubjectFull: Yersinia pseudotuberculosis
        Type: general
      – SubjectFull: Operons
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      – SubjectFull: Pseudotuberculosis
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      – SubjectFull: Chromosomes
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      – SubjectFull: Polymyxin
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      – SubjectFull: Salmonella enteritidis
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      – SubjectFull: Mass spectrometry
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      – SubjectFull: Microbiology
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      – TitleFull: The pmrF polymyxin-resistance operon of Yersinia pseudotuberculosis is upregulated by the Pho-P-PhoQ two-component system but not by PmrA-PmrB, and is not required for virulence.
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              Text: Dec2004
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