A novel process for the production of a veterinary rabies vaccine in BHK-21 cells grown on microcarriers in a 20-l bioreactor.
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| Title: | A novel process for the production of a veterinary rabies vaccine in BHK-21 cells grown on microcarriers in a 20-l bioreactor. |
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| Authors: | Kallel, H.1 hela.kallel@pasteur.rns.tn, Rourou, S.1, Majoul, S.1, Loukil, H.1 |
| Source: | Applied Microbiology & Biotechnology. Jun2003, Vol. 61 Issue 5/6, p441-446. 6p. 6 Graphs. |
| Subjects: | Rabies vaccines, Veterinary medicine, Vaccines, Bioreactors, Rabies, Amino acids |
| Abstract: | We studied BHK-21 cells growth in a 2-l bioreactor and investigated the effects of microcarrier concentration, type of growth medium, culture mode and serum concentration. The highest cell density reached was equal to 4×106 cells/ml and was achieved in minimum essential medium supplemented with Hanks' salts, non-essential amino acids and 5% fetal calf serum, using a perfusion culture mode and a microcarrier concentration of 4 g Cytodex 3/l. We studied rabies virus production (PV/BHK-21 strain) by BHK-21 cells grown at the optimal conditions determined previously. We analyzed the effects of multiplicity of infection (MOI) and type of medium used for virus multiplication in spinner-flasks and showed that the highest virus titer reached (when the cells were infected at a MOI of 0.3) in M199 medium supplemented with 0.2% of bovine serum albumin was equal to 8.2×107 Fluorescent Focus Units (FFU)/ml. When we grew the cells in a 2-l perfused bioreactor, we obtained a maximal virus titer of 3×108 FFU/ml. In addition, we scaled-up to a 20-l bioreactor and obtained similar results for cell density and virus titer. The experimental vaccine we developed meets WHO requirements for vaccine potency. Each run yielded about 40,000 doses of potent vaccine. [ABSTRACT FROM AUTHOR] |
| Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 15681439 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: A novel process for the production of a veterinary rabies vaccine in BHK-21 cells grown on microcarriers in a 20-l bioreactor. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Kallel%2C+H%2E%22">Kallel, H.</searchLink><relatesTo>1</relatesTo><i> hela.kallel@pasteur.rns.tn</i><br /><searchLink fieldCode="AR" term="%22Rourou%2C+S%2E%22">Rourou, S.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Majoul%2C+S%2E%22">Majoul, S.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Loukil%2C+H%2E%22">Loukil, H.</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. Jun2003, Vol. 61 Issue 5/6, p441-446. 6p. 6 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Rabies+vaccines%22">Rabies vaccines</searchLink><br /><searchLink fieldCode="DE" term="%22Veterinary+medicine%22">Veterinary medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Vaccines%22">Vaccines</searchLink><br /><searchLink fieldCode="DE" term="%22Bioreactors%22">Bioreactors</searchLink><br /><searchLink fieldCode="DE" term="%22Rabies%22">Rabies</searchLink><br /><searchLink fieldCode="DE" term="%22Amino+acids%22">Amino acids</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: We studied BHK-21 cells growth in a 2-l bioreactor and investigated the effects of microcarrier concentration, type of growth medium, culture mode and serum concentration. The highest cell density reached was equal to 4×106 cells/ml and was achieved in minimum essential medium supplemented with Hanks' salts, non-essential amino acids and 5% fetal calf serum, using a perfusion culture mode and a microcarrier concentration of 4 g Cytodex 3/l. We studied rabies virus production (PV/BHK-21 strain) by BHK-21 cells grown at the optimal conditions determined previously. We analyzed the effects of multiplicity of infection (MOI) and type of medium used for virus multiplication in spinner-flasks and showed that the highest virus titer reached (when the cells were infected at a MOI of 0.3) in M199 medium supplemented with 0.2% of bovine serum albumin was equal to 8.2×107 Fluorescent Focus Units (FFU)/ml. When we grew the cells in a 2-l perfused bioreactor, we obtained a maximal virus titer of 3×108 FFU/ml. In addition, we scaled-up to a 20-l bioreactor and obtained similar results for cell density and virus titer. The experimental vaccine we developed meets WHO requirements for vaccine potency. Each run yielded about 40,000 doses of potent vaccine. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00253-003-1245-3 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 441 Subjects: – SubjectFull: Rabies vaccines Type: general – SubjectFull: Veterinary medicine Type: general – SubjectFull: Vaccines Type: general – SubjectFull: Bioreactors Type: general – SubjectFull: Rabies Type: general – SubjectFull: Amino acids Type: general Titles: – TitleFull: A novel process for the production of a veterinary rabies vaccine in BHK-21 cells grown on microcarriers in a 20-l bioreactor. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Kallel, H. – PersonEntity: Name: NameFull: Rourou, S. – PersonEntity: Name: NameFull: Majoul, S. – PersonEntity: Name: NameFull: Loukil, H. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: Jun2003 Type: published Y: 2003 Identifiers: – Type: issn-print Value: 01757598 Numbering: – Type: volume Value: 61 – Type: issue Value: 5/6 Titles: – TitleFull: Applied Microbiology & Biotechnology Type: main |
| ResultId | 1 |
