Verification of lateral flow antigen tests for SARS-CoV-2 by qPCR directly from the test device.
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| Title: | Verification of lateral flow antigen tests for SARS-CoV-2 by qPCR directly from the test device. |
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| Authors: | Czibere, Ludwig1 (AUTHOR), Burggraf, Siegfried1 (AUTHOR), Becker, Marc1,2 (AUTHOR), Durner, Jürgen1,2 (AUTHOR) j.durner@labor-becker.de, Draenert, Miriam E.1,2 (AUTHOR) |
| Source: | Dental Materials. Jun2022, Vol. 38 Issue 6, pe155-e159. 5p. |
| Subjects: | COVID-19 testing, Point-of-care testing, COVID-19 pandemic, Dental emergencies, Amplification reactions, Medical screening |
| Abstract: | Fast and reliable detection of infection is a key to control the SARS-CoV-2 pandemic. Lateral flow antigen tests (LFATs) are inexpensive, easy to use, but have to be verified, as they are rather unspecific and can produce both, false positive and false negative results. Our objective was to combine the speed of LFAT for SARS-CoV-2 with the reliability of qPCR tests. A serial dilution of a patient sample positive for SARS-CoV-2 was prepared and added to LFAT wells from two manufacturers. After evaluation, the devices were opened, the strips removed and extracted in a solution. Amplification was performed using point of care PCR systems (cobas® Liat®, ID NOW™) or on a LightCycler after extraction by MagNAPure 96. The nucleic acid amplification systems yielded higher sensitivity to LFAT. Thus, all samples determined positive by LFAT from the serial dilution were also positive in the subsequent amplification reactions. Sensitivity using extracted eluates was 10–100 times higher. The usage of LFAT is highly recommended for single samples in emergency dental or emergency clinical settings, for smaller cohorts, or even for larger population screening, as it is inexpensive and fast. Positive results can be conveniently verified directly from the test devices using either point of care test equipment or more complex laboratory equipment thus making a major impact on efficient management of infections and isolations. [ABSTRACT FROM AUTHOR] |
| Copyright of Dental Materials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 157076020 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Verification of lateral flow antigen tests for SARS-CoV-2 by qPCR directly from the test device. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Czibere%2C+Ludwig%22">Czibere, Ludwig</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Burggraf%2C+Siegfried%22">Burggraf, Siegfried</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Becker%2C+Marc%22">Becker, Marc</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Durner%2C+Jürgen%22">Durner, Jürgen</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> j.durner@labor-becker.de</i><br /><searchLink fieldCode="AR" term="%22Draenert%2C+Miriam+E%2E%22">Draenert, Miriam E.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Dental+Materials%22">Dental Materials</searchLink>. Jun2022, Vol. 38 Issue 6, pe155-e159. 5p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22COVID-19+testing%22">COVID-19 testing</searchLink><br /><searchLink fieldCode="DE" term="%22Point-of-care+testing%22">Point-of-care testing</searchLink><br /><searchLink fieldCode="DE" term="%22COVID-19+pandemic%22">COVID-19 pandemic</searchLink><br /><searchLink fieldCode="DE" term="%22Dental+emergencies%22">Dental emergencies</searchLink><br /><searchLink fieldCode="DE" term="%22Amplification+reactions%22">Amplification reactions</searchLink><br /><searchLink fieldCode="DE" term="%22Medical+screening%22">Medical screening</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Fast and reliable detection of infection is a key to control the SARS-CoV-2 pandemic. Lateral flow antigen tests (LFATs) are inexpensive, easy to use, but have to be verified, as they are rather unspecific and can produce both, false positive and false negative results. Our objective was to combine the speed of LFAT for SARS-CoV-2 with the reliability of qPCR tests. A serial dilution of a patient sample positive for SARS-CoV-2 was prepared and added to LFAT wells from two manufacturers. After evaluation, the devices were opened, the strips removed and extracted in a solution. Amplification was performed using point of care PCR systems (cobas® Liat®, ID NOW™) or on a LightCycler after extraction by MagNAPure 96. The nucleic acid amplification systems yielded higher sensitivity to LFAT. Thus, all samples determined positive by LFAT from the serial dilution were also positive in the subsequent amplification reactions. Sensitivity using extracted eluates was 10–100 times higher. The usage of LFAT is highly recommended for single samples in emergency dental or emergency clinical settings, for smaller cohorts, or even for larger population screening, as it is inexpensive and fast. Positive results can be conveniently verified directly from the test devices using either point of care test equipment or more complex laboratory equipment thus making a major impact on efficient management of infections and isolations. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Dental Materials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.dental.2022.03.005 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 5 StartPage: e155 Subjects: – SubjectFull: COVID-19 testing Type: general – SubjectFull: Point-of-care testing Type: general – SubjectFull: COVID-19 pandemic Type: general – SubjectFull: Dental emergencies Type: general – SubjectFull: Amplification reactions Type: general – SubjectFull: Medical screening Type: general Titles: – TitleFull: Verification of lateral flow antigen tests for SARS-CoV-2 by qPCR directly from the test device. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Czibere, Ludwig – PersonEntity: Name: NameFull: Burggraf, Siegfried – PersonEntity: Name: NameFull: Becker, Marc – PersonEntity: Name: NameFull: Durner, Jürgen – PersonEntity: Name: NameFull: Draenert, Miriam E. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: Jun2022 Type: published Y: 2022 Identifiers: – Type: issn-print Value: 01095641 Numbering: – Type: volume Value: 38 – Type: issue Value: 6 Titles: – TitleFull: Dental Materials Type: main |
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