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DNA transfer by highly asymmetric somatic hybridisation in Medicago truncatula (+) Medicago rugosa and Medicago truncatula (+) Medicago scutellata.

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Title: DNA transfer by highly asymmetric somatic hybridisation in Medicago truncatula (+) Medicago rugosa and Medicago truncatula (+) Medicago scutellata.
Authors: Tian, D.1, Niu, C.1, Rose, R.J.1 birjr@alinga.newcastle.edu.au
Source: Theoretical & Applied Genetics. Jan2002, Vol. 104 Issue 1, p9-16. 8p.
Subjects: Medicago, Legumes, Cell nuclei, Plant genetics, DNA, Southern blot
Abstract: A regenerable line of Medicago truncatula (Jemalong 2HA) as a recipient species, was fused with the sexually incompatible species Medicago scutellata or Medicago rugosa. The treatments described maintain the chromosome number of the recipient but enable the transfer of small amounts of DNA of the donor species, probably by intergenomic recombination. Without a chromosome number-change fusion products can readily regenerate to produce fertile plants; and potentially a library with a diverse array of new genetic material. The selection of fused cells is based on treatment of the recipient cells with iodoacetamide (IOA), a non-regenerable donor, γ-irradiation of the donor, and regeneration on a medium favouring the recipient. DNA transfer was demonstrated by amplified fragment length polymorphism (AFLP), Southern hybridisation and changed morphology. [ABSTRACT FROM AUTHOR]
Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: DNA transfer by highly asymmetric somatic hybridisation in Medicago truncatula (+) Medicago rugosa and Medicago truncatula (+) Medicago scutellata.
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  Data: <searchLink fieldCode="AR" term="%22Tian%2C+D%2E%22">Tian, D.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Niu%2C+C%2E%22">Niu, C.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Rose%2C+R%2EJ%2E%22">Rose, R.J.</searchLink><relatesTo>1</relatesTo><i> birjr@alinga.newcastle.edu.au</i>
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  Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. Jan2002, Vol. 104 Issue 1, p9-16. 8p.
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  Data: <searchLink fieldCode="DE" term="%22Medicago%22">Medicago</searchLink><br /><searchLink fieldCode="DE" term="%22Legumes%22">Legumes</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+nuclei%22">Cell nuclei</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+genetics%22">Plant genetics</searchLink><br /><searchLink fieldCode="DE" term="%22DNA%22">DNA</searchLink><br /><searchLink fieldCode="DE" term="%22Southern+blot%22">Southern blot</searchLink>
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  Data: A regenerable line of Medicago truncatula (Jemalong 2HA) as a recipient species, was fused with the sexually incompatible species Medicago scutellata or Medicago rugosa. The treatments described maintain the chromosome number of the recipient but enable the transfer of small amounts of DNA of the donor species, probably by intergenomic recombination. Without a chromosome number-change fusion products can readily regenerate to produce fertile plants; and potentially a library with a diverse array of new genetic material. The selection of fused cells is based on treatment of the recipient cells with iodoacetamide (IOA), a non-regenerable donor, γ-irradiation of the donor, and regeneration on a medium favouring the recipient. DNA transfer was demonstrated by amplified fragment length polymorphism (AFLP), Southern hybridisation and changed morphology. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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