AFLP markers tightly linked to the aluminum-tolerance gene Alt3 in rye (Secale cereale L.).
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| Title: | AFLP markers tightly linked to the aluminum-tolerance gene Alt3 in rye (Secale cereale L.). |
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| Authors: | Miftahudin1, Scoles, G. J.2, Gustafson, J. P.3 pgus@missouri.edu |
| Source: | Theoretical & Applied Genetics. Mar2002, Vol. 104 Issue 4, p626-631. 6p. |
| Subjects: | Rye, Triticale, Plants, Plant genetics, Genetic polymorphisms, Nucleic acids, Genetics |
| Abstract: | Rye (Secale cereale L.) is considered to be the most aluminum (Al)-tolerant species among the Triticeae. It has been suggested that aluminum tolerance in rye is controlled by three major genes (Alt genes) located on rye chromosome arms 3RL, 4RL, and 6RS, respectively. Screening of an F6 rye recombinant inbred line (RIL) population derived from the cross between an Al-tolerant rye (M39A-1–6) and an Al-sensitive rye (M77A-1) showed that a single gene controls aluminum tolerance in the population analyzed. In order to identify molecular markers tightly linked to the gene, we used a combination of amplified fragment length polymorphism (AFLP) and bulked segregant analysis techniques to evaluate the F6 rye RIL population. We analyzed approximately 22,500 selectively amplified DNA fragments using 204 primer combinations and identified three AFLP markers tightly linked to the Alt gene. Two of these markers flanked the Alt locus at distance of 0.4 and 0.7 cM. Chromosomal localization using cloned AFLP and a restriction fragment length polymorphism (RFLP) marker indicated that the gene was on the long arm of rye chromosome 4R. The RFLP marker (BCD1230) co-segregated with the Alt gene. Since the gene is on chromosome 4R, the gene was designated as Alt3. These markers are being used as a starting point in the construction of a high resolution map of the Alt3 region in rye. [ABSTRACT FROM AUTHOR] |
| Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Items | – Name: Title Label: Title Group: Ti Data: AFLP markers tightly linked to the aluminum-tolerance gene Alt3 in rye (Secale cereale L.). – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Miftahudin%22">Miftahudin</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Scoles%2C+G%2E+J%2E%22">Scoles, G. J.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Gustafson%2C+J%2E+P%2E%22">Gustafson, J. P.</searchLink><relatesTo>3</relatesTo><i> pgus@missouri.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. Mar2002, Vol. 104 Issue 4, p626-631. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Rye%22">Rye</searchLink><br /><searchLink fieldCode="DE" term="%22Triticale%22">Triticale</searchLink><br /><searchLink fieldCode="DE" term="%22Plants%22">Plants</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+genetics%22">Plant genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+polymorphisms%22">Genetic polymorphisms</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleic+acids%22">Nucleic acids</searchLink><br /><searchLink fieldCode="DE" term="%22Genetics%22">Genetics</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Rye (Secale cereale L.) is considered to be the most aluminum (Al)-tolerant species among the Triticeae. It has been suggested that aluminum tolerance in rye is controlled by three major genes (Alt genes) located on rye chromosome arms 3RL, 4RL, and 6RS, respectively. Screening of an F6 rye recombinant inbred line (RIL) population derived from the cross between an Al-tolerant rye (M39A-1–6) and an Al-sensitive rye (M77A-1) showed that a single gene controls aluminum tolerance in the population analyzed. In order to identify molecular markers tightly linked to the gene, we used a combination of amplified fragment length polymorphism (AFLP) and bulked segregant analysis techniques to evaluate the F6 rye RIL population. We analyzed approximately 22,500 selectively amplified DNA fragments using 204 primer combinations and identified three AFLP markers tightly linked to the Alt gene. Two of these markers flanked the Alt locus at distance of 0.4 and 0.7 cM. Chromosomal localization using cloned AFLP and a restriction fragment length polymorphism (RFLP) marker indicated that the gene was on the long arm of rye chromosome 4R. The RFLP marker (BCD1230) co-segregated with the Alt gene. Since the gene is on chromosome 4R, the gene was designated as Alt3. These markers are being used as a starting point in the construction of a high resolution map of the Alt3 region in rye. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00122-001-0782-3 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 626 Subjects: – SubjectFull: Rye Type: general – SubjectFull: Triticale Type: general – SubjectFull: Plants Type: general – SubjectFull: Plant genetics Type: general – SubjectFull: Genetic polymorphisms Type: general – SubjectFull: Nucleic acids Type: general – SubjectFull: Genetics Type: general Titles: – TitleFull: AFLP markers tightly linked to the aluminum-tolerance gene Alt3 in rye (Secale cereale L.). Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Miftahudin – PersonEntity: Name: NameFull: Scoles, G. J. – PersonEntity: Name: NameFull: Gustafson, J. P. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2002 Type: published Y: 2002 Identifiers: – Type: issn-print Value: 00405752 Numbering: – Type: volume Value: 104 – Type: issue Value: 4 Titles: – TitleFull: Theoretical & Applied Genetics Type: main |
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