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Prussian Blue Nanozymes with Enhanced Catalytic Activity: Size Tuning and Application in ELISA-like Immunoassay.

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Title: Prussian Blue Nanozymes with Enhanced Catalytic Activity: Size Tuning and Application in ELISA-like Immunoassay.
Authors: Khramtsov, Pavel1,2 (AUTHOR) khramtsovpavel@yandex.ru, Kropaneva, Maria1,2 (AUTHOR) krasnykh-m@mail.ru, Minin, Artem3,4 (AUTHOR) calamatica@gmail.com, Bochkova, Maria1,2 (AUTHOR) mantissa7@mail.ru, Timganova, Valeria2 (AUTHOR), Maximov, Andrey5 (AUTHOR) htb03starosta@gmail.com, Puzik, Alexey6,7,8,9 (AUTHOR) alex.puzik@mail.ru, Zamorina, Svetlana1,2 (AUTHOR) mraev@iegm.ru, Rayev, Mikhail1,2 (AUTHOR)
Source: Nanomaterials (2079-4991). May2022, Vol. 12 Issue 10, p1630-1630. 21p.
Subjects: Prussian blue, Synthetic enzymes, Catalytic activity, Immunoassay, Prostate-specific antigen, Peroxidase
Abstract: Prussian blue nanozymes possessing peroxidase-like activity gather significant attention as alternatives to natural enzymes in therapy, biosensing, and environmental remediation. Recently, Prussian blue nanoparticles with enhanced catalytic activity prepared by reduction of FeCl3/K3[Fe(CN)6] mixture have been reported. These nanoparticles were denoted as 'artificial peroxidase' nanozymes. Our study provides insights into the process of their synthesis. We studied how the size of nanozymes and synthesis yield can be controlled via adjustment of the synthesis conditions. Based on these results, we developed a reproducible and scalable method for the preparation of 'artificial peroxidase' with tunable sizes and enhanced catalytic activity. Nanozymes modified with gelatin shell and functionalized with affine molecules were applied as labels in colorimetric immunoassays of prostate-specific antigen and tetanus antibodies, enabling detection of these analytes in the range of clinically relevant concentrations. Protein coating provides excellent colloidal stability of nanozymes in physiological conditions and stability upon long-term storage. [ABSTRACT FROM AUTHOR]
Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Prussian Blue Nanozymes with Enhanced Catalytic Activity: Size Tuning and Application in ELISA-like Immunoassay.
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  Data: <searchLink fieldCode="AR" term="%22Khramtsov%2C+Pavel%22">Khramtsov, Pavel</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> khramtsovpavel@yandex.ru</i><br /><searchLink fieldCode="AR" term="%22Kropaneva%2C+Maria%22">Kropaneva, Maria</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> krasnykh-m@mail.ru</i><br /><searchLink fieldCode="AR" term="%22Minin%2C+Artem%22">Minin, Artem</searchLink><relatesTo>3,4</relatesTo> (AUTHOR)<i> calamatica@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Bochkova%2C+Maria%22">Bochkova, Maria</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> mantissa7@mail.ru</i><br /><searchLink fieldCode="AR" term="%22Timganova%2C+Valeria%22">Timganova, Valeria</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Maximov%2C+Andrey%22">Maximov, Andrey</searchLink><relatesTo>5</relatesTo> (AUTHOR)<i> htb03starosta@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Puzik%2C+Alexey%22">Puzik, Alexey</searchLink><relatesTo>6,7,8,9</relatesTo> (AUTHOR)<i> alex.puzik@mail.ru</i><br /><searchLink fieldCode="AR" term="%22Zamorina%2C+Svetlana%22">Zamorina, Svetlana</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> mraev@iegm.ru</i><br /><searchLink fieldCode="AR" term="%22Rayev%2C+Mikhail%22">Rayev, Mikhail</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Nanomaterials+%282079-4991%29%22">Nanomaterials (2079-4991)</searchLink>. May2022, Vol. 12 Issue 10, p1630-1630. 21p.
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  Data: <searchLink fieldCode="DE" term="%22Prussian+blue%22">Prussian blue</searchLink><br /><searchLink fieldCode="DE" term="%22Synthetic+enzymes%22">Synthetic enzymes</searchLink><br /><searchLink fieldCode="DE" term="%22Catalytic+activity%22">Catalytic activity</searchLink><br /><searchLink fieldCode="DE" term="%22Immunoassay%22">Immunoassay</searchLink><br /><searchLink fieldCode="DE" term="%22Prostate-specific+antigen%22">Prostate-specific antigen</searchLink><br /><searchLink fieldCode="DE" term="%22Peroxidase%22">Peroxidase</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Prussian blue nanozymes possessing peroxidase-like activity gather significant attention as alternatives to natural enzymes in therapy, biosensing, and environmental remediation. Recently, Prussian blue nanoparticles with enhanced catalytic activity prepared by reduction of FeCl3/K3[Fe(CN)6] mixture have been reported. These nanoparticles were denoted as 'artificial peroxidase' nanozymes. Our study provides insights into the process of their synthesis. We studied how the size of nanozymes and synthesis yield can be controlled via adjustment of the synthesis conditions. Based on these results, we developed a reproducible and scalable method for the preparation of 'artificial peroxidase' with tunable sizes and enhanced catalytic activity. Nanozymes modified with gelatin shell and functionalized with affine molecules were applied as labels in colorimetric immunoassays of prostate-specific antigen and tetanus antibodies, enabling detection of these analytes in the range of clinically relevant concentrations. Protein coating provides excellent colloidal stability of nanozymes in physiological conditions and stability upon long-term storage. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.3390/nano12101630
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      – Code: eng
        Text: English
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        PageCount: 21
        StartPage: 1630
    Subjects:
      – SubjectFull: Prussian blue
        Type: general
      – SubjectFull: Synthetic enzymes
        Type: general
      – SubjectFull: Catalytic activity
        Type: general
      – SubjectFull: Immunoassay
        Type: general
      – SubjectFull: Prostate-specific antigen
        Type: general
      – SubjectFull: Peroxidase
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    Titles:
      – TitleFull: Prussian Blue Nanozymes with Enhanced Catalytic Activity: Size Tuning and Application in ELISA-like Immunoassay.
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            – D: 15
              M: 05
              Text: May2022
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