On chloroplast DNA variations in the olive (Olea europaea L.) complex: comparison of RFLP and PCR polymorphisms.
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| Title: | On chloroplast DNA variations in the olive (Olea europaea L.) complex: comparison of RFLP and PCR polymorphisms. |
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| Authors: | Besnard, G.1, Bervillé, A.1 berville@ensam.inra.fr |
| Source: | Theoretical & Applied Genetics. May2002, Vol. 104 Issue 6/7, p1157-1163. 7p. |
| Subjects: | Olive, Chloroplast DNA, Herbaria, Polymerase chain reaction, Restriction fragment length polymorphisms, Plant genetics |
| Abstract: | Previous papers have dealt with olive chloroplastic DNA (cpDNA) variation revealed using several methods (RFLPs, PCR-RFLPs and microsatellites) and have led to different conclusions. This paper aims to reconsider these divergences. A Southern approach was applied to reveal polymorphism. We used chloroplast DNA of Phillyrea media as a probe. Based on these data, only four chlorotypes were identified in the olive complex. The number of detected lineages was lower than reported in the literature using a direct cpDNA RFLP approach, and was insufficient to distinguish the North African subspecies europaea, maroccana, guanchica and laperrinei. Furthermore, one individual considered belonging to the subspecies laperrinei was questionable. Using other cpDNA and mitochondrial DNA (mtDNA) polymorphisms – based on PCR and RFLP methods, respectively – we showed that this individual displays the cytoplasmic lineage CE1-ME1 characteristic of most Eastern mediterranean cultivars and of Olea europaea subsp. laperrinei from Hoggar. However, based on RAPDs, this individual appeared as mislabelled and probably corresponded to a Mediterranean cultivar or a feral form. In addition, we checked O. e. subsp. laperrinei herbarium samples using two cpDNA microsatellites, which revealed polymorphisms. These also supported that both populations from Niger and Algeria displayed a chlorotype related to CE1. Consequently, based on cpDNA, the relationships of O. e. subsp. laperrinei from Hoggar with a Mediterranean lineage appeared well supported, whereas the South West Moroccan and Macaronesian olives appeared in a different clade using both mtDNA and cpDNA polymorphisms. We conclude that methods based on PCR reveal more polymorphisms in the cpDNA and lead to more-reliable results that the classical RFLP method. [ABSTRACT FROM AUTHOR] |
| Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Items | – Name: Title Label: Title Group: Ti Data: On chloroplast DNA variations in the olive (Olea europaea L.) complex: comparison of RFLP and PCR polymorphisms. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Besnard%2C+G%2E%22">Besnard, G.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Bervillé%2C+A%2E%22">Bervillé, A.</searchLink><relatesTo>1</relatesTo><i> berville@ensam.inra.fr</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. May2002, Vol. 104 Issue 6/7, p1157-1163. 7p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Olive%22">Olive</searchLink><br /><searchLink fieldCode="DE" term="%22Chloroplast+DNA%22">Chloroplast DNA</searchLink><br /><searchLink fieldCode="DE" term="%22Herbaria%22">Herbaria</searchLink><br /><searchLink fieldCode="DE" term="%22Polymerase+chain+reaction%22">Polymerase chain reaction</searchLink><br /><searchLink fieldCode="DE" term="%22Restriction+fragment+length+polymorphisms%22">Restriction fragment length polymorphisms</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+genetics%22">Plant genetics</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Previous papers have dealt with olive chloroplastic DNA (cpDNA) variation revealed using several methods (RFLPs, PCR-RFLPs and microsatellites) and have led to different conclusions. This paper aims to reconsider these divergences. A Southern approach was applied to reveal polymorphism. We used chloroplast DNA of Phillyrea media as a probe. Based on these data, only four chlorotypes were identified in the olive complex. The number of detected lineages was lower than reported in the literature using a direct cpDNA RFLP approach, and was insufficient to distinguish the North African subspecies europaea, maroccana, guanchica and laperrinei. Furthermore, one individual considered belonging to the subspecies laperrinei was questionable. Using other cpDNA and mitochondrial DNA (mtDNA) polymorphisms – based on PCR and RFLP methods, respectively – we showed that this individual displays the cytoplasmic lineage CE1-ME1 characteristic of most Eastern mediterranean cultivars and of Olea europaea subsp. laperrinei from Hoggar. However, based on RAPDs, this individual appeared as mislabelled and probably corresponded to a Mediterranean cultivar or a feral form. In addition, we checked O. e. subsp. laperrinei herbarium samples using two cpDNA microsatellites, which revealed polymorphisms. These also supported that both populations from Niger and Algeria displayed a chlorotype related to CE1. Consequently, based on cpDNA, the relationships of O. e. subsp. laperrinei from Hoggar with a Mediterranean lineage appeared well supported, whereas the South West Moroccan and Macaronesian olives appeared in a different clade using both mtDNA and cpDNA polymorphisms. We conclude that methods based on PCR reveal more polymorphisms in the cpDNA and lead to more-reliable results that the classical RFLP method. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00122-001-0834-8 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 1157 Subjects: – SubjectFull: Olive Type: general – SubjectFull: Chloroplast DNA Type: general – SubjectFull: Herbaria Type: general – SubjectFull: Polymerase chain reaction Type: general – SubjectFull: Restriction fragment length polymorphisms Type: general – SubjectFull: Plant genetics Type: general Titles: – TitleFull: On chloroplast DNA variations in the olive (Olea europaea L.) complex: comparison of RFLP and PCR polymorphisms. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Besnard, G. – PersonEntity: Name: NameFull: Bervillé, A. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 05 Text: May2002 Type: published Y: 2002 Identifiers: – Type: issn-print Value: 00405752 Numbering: – Type: volume Value: 104 – Type: issue Value: 6/7 Titles: – TitleFull: Theoretical & Applied Genetics Type: main |
| ResultId | 1 |
