Assessment of the C4 phosphoenolpyruvate carboxylase gene diversity in grasses (Poaceae).
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| Title: | Assessment of the C |
|---|---|
| Authors: | Besnard, G.1 gbesnar@hotmail.com, Offmann, B.1, Robert, C.1, Rouch, C.1, Cadet, F.1 |
| Source: | Theoretical & Applied Genetics. Aug2002, Vol. 105 Issue 2/3, p404-412. 9p. |
| Subjects: | Grasses, Plant enzymes, Polymerase chain reaction, Gene amplification, Gene expression, Plant genetics |
| Abstract: | C4 phosphoenolpyruvate carboxylase (PEPC) is a key enzyme in the C4 photosynthetic pathway. To analyze the diversity of the corresponding gene in grasses, we designed PCR primers to specifically amplify C4 PEPC cDNA fragments. Using RT-PCR, we generated partial PEPC cDNA sequences in several grasses displaying a C4 photosynthetic pathway. All these sequences displayed a high homology (78–99%) with known grass C4 PEPCs. PCR amplification did not occur in two grasses that display the C3 photosynthetic pathway, and therefore we assumed that all generated sequences corresponded to C4 PEPC transcripts. Based on one large cDNA segment, phylogenetic reconstruction enabled us to assess the relationships between 22 grass species belonging to the subfamilies Panicoideae, Arundinoideae and Chloridoideae. The phylogenetic relationships between species deduced from C4 PEPC sequences were similar to those deduced from other molecular data. The sequence evolution of the C4 PEPC isoform was faster than in the other PEPC isoforms. Finally, the utility of the C4 PEPC gene phylogeny to study the evolution of C4 photosynthesis in grasses is discussed. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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