View in EDS HTML Full Text PDF Full Text

Establishment of effective plantlets regeneration protocol via isolated microspore culture in Malaysian indica rice MR219.

Saved in:
Bibliographic Details
Title: Establishment of effective plantlets regeneration protocol via isolated microspore culture in Malaysian indica rice MR219.
Authors: Rahman, Zuraida Abd1 (AUTHOR) azuraida@mardi.gov.my, Seman, Zulkifli Ahmad1 (AUTHOR), Othman, Ayu Nazreena1 (AUTHOR), Ghaffar, Mohamad Bahagia Ab2 (AUTHOR), Razak, Shahril Ab3 (AUTHOR), Yusof, Muhammad Fairuz Mohd3 (AUTHOR), Nasir, Khairun Hisam3 (AUTHOR), Ahmad, Khairulmazmi4 (AUTHOR), Chow, Yeow Lit5 (AUTHOR), How, Teo Chee6 (AUTHOR), Saad, Norsharina Md7 (AUTHOR), Subramaniam, Sreeramanan5,8,9 (AUTHOR) sreeramanan@gmail.com
Source: Plant Biotechnology Reports. Jun2022, Vol. 16 Issue 3, p343-355. 13p.
Subjects: Callus, Kinetin, Rice, Centrifugation, Sucrose, Callus (Botany)
Abstract: The current study recognised the issues encountered in regenerating Malaysia MR219 rice plantlet via microspore culture and attempted to develop an efficient protocol in overcoming the restraints. In the present study, a high proportion of uninucleate microspores (49.17%) was isolated from Stage 2-Segment II panicle (59–61 days), which also exhibited the highest callus initiation rate of 8.50%. Maintenance of the panicles under a cool temperature of 4 °C for 7 days before isolating the microspores, resulted in the highest microspore viability of 58.33% and callus initiation rate of 9.33%. The microspore isolation protocol was also optimised in the present study. The filtration sieve engagement with a pore size of 80 µm and further suspension centrifugation at 800 rpm for 5 min produced the highest microspore viability percentage and callus initiation rate. The incorporation of 3.0 mg/L kinetin in conjunction with 0.5 mg/L 2,4-D greatly enhanced the callus initiation rate, with 11.33%. The callus proliferation capacity, with the formation of 481.67 mg callus, was significantly promoted by the addition of 1.0 mg/L kinetin and 0.5 mg/L 2,4-D into the growth medium. Moreover, a higher green plantlet regeneration frequency of 2.83% was induced by the supplementation of 8% sucrose, which produced an average of 3.50 green plantlets. [ABSTRACT FROM AUTHOR]
Copyright of Plant Biotechnology Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: egs
DbLabel: Engineering Source
An: 157279349
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Establishment of effective plantlets regeneration protocol via isolated microspore culture in Malaysian indica rice MR219.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Rahman%2C+Zuraida+Abd%22">Rahman, Zuraida Abd</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> azuraida@mardi.gov.my</i><br /><searchLink fieldCode="AR" term="%22Seman%2C+Zulkifli+Ahmad%22">Seman, Zulkifli Ahmad</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Othman%2C+Ayu+Nazreena%22">Othman, Ayu Nazreena</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ghaffar%2C+Mohamad+Bahagia+Ab%22">Ghaffar, Mohamad Bahagia Ab</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Razak%2C+Shahril+Ab%22">Razak, Shahril Ab</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yusof%2C+Muhammad+Fairuz+Mohd%22">Yusof, Muhammad Fairuz Mohd</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Nasir%2C+Khairun+Hisam%22">Nasir, Khairun Hisam</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ahmad%2C+Khairulmazmi%22">Ahmad, Khairulmazmi</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chow%2C+Yeow+Lit%22">Chow, Yeow Lit</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22How%2C+Teo+Chee%22">How, Teo Chee</searchLink><relatesTo>6</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Saad%2C+Norsharina+Md%22">Saad, Norsharina Md</searchLink><relatesTo>7</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Subramaniam%2C+Sreeramanan%22">Subramaniam, Sreeramanan</searchLink><relatesTo>5,8,9</relatesTo> (AUTHOR)<i> sreeramanan@gmail.com</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Plant+Biotechnology+Reports%22">Plant Biotechnology Reports</searchLink>. Jun2022, Vol. 16 Issue 3, p343-355. 13p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Callus%22">Callus</searchLink><br /><searchLink fieldCode="DE" term="%22Kinetin%22">Kinetin</searchLink><br /><searchLink fieldCode="DE" term="%22Rice%22">Rice</searchLink><br /><searchLink fieldCode="DE" term="%22Centrifugation%22">Centrifugation</searchLink><br /><searchLink fieldCode="DE" term="%22Sucrose%22">Sucrose</searchLink><br /><searchLink fieldCode="DE" term="%22Callus+%28Botany%29%22">Callus (Botany)</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The current study recognised the issues encountered in regenerating Malaysia MR219 rice plantlet via microspore culture and attempted to develop an efficient protocol in overcoming the restraints. In the present study, a high proportion of uninucleate microspores (49.17%) was isolated from Stage 2-Segment II panicle (59–61 days), which also exhibited the highest callus initiation rate of 8.50%. Maintenance of the panicles under a cool temperature of 4 °C for 7 days before isolating the microspores, resulted in the highest microspore viability of 58.33% and callus initiation rate of 9.33%. The microspore isolation protocol was also optimised in the present study. The filtration sieve engagement with a pore size of 80 µm and further suspension centrifugation at 800 rpm for 5 min produced the highest microspore viability percentage and callus initiation rate. The incorporation of 3.0 mg/L kinetin in conjunction with 0.5 mg/L 2,4-D greatly enhanced the callus initiation rate, with 11.33%. The callus proliferation capacity, with the formation of 481.67 mg callus, was significantly promoted by the addition of 1.0 mg/L kinetin and 0.5 mg/L 2,4-D into the growth medium. Moreover, a higher green plantlet regeneration frequency of 2.83% was induced by the supplementation of 8% sucrose, which produced an average of 3.50 green plantlets. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Plant Biotechnology Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=157279349
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1007/s11816-022-00742-4
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 13
        StartPage: 343
    Subjects:
      – SubjectFull: Callus
        Type: general
      – SubjectFull: Kinetin
        Type: general
      – SubjectFull: Rice
        Type: general
      – SubjectFull: Centrifugation
        Type: general
      – SubjectFull: Sucrose
        Type: general
      – SubjectFull: Callus (Botany)
        Type: general
    Titles:
      – TitleFull: Establishment of effective plantlets regeneration protocol via isolated microspore culture in Malaysian indica rice MR219.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Rahman, Zuraida Abd
      – PersonEntity:
          Name:
            NameFull: Seman, Zulkifli Ahmad
      – PersonEntity:
          Name:
            NameFull: Othman, Ayu Nazreena
      – PersonEntity:
          Name:
            NameFull: Ghaffar, Mohamad Bahagia Ab
      – PersonEntity:
          Name:
            NameFull: Razak, Shahril Ab
      – PersonEntity:
          Name:
            NameFull: Yusof, Muhammad Fairuz Mohd
      – PersonEntity:
          Name:
            NameFull: Nasir, Khairun Hisam
      – PersonEntity:
          Name:
            NameFull: Ahmad, Khairulmazmi
      – PersonEntity:
          Name:
            NameFull: Chow, Yeow Lit
      – PersonEntity:
          Name:
            NameFull: How, Teo Chee
      – PersonEntity:
          Name:
            NameFull: Saad, Norsharina Md
      – PersonEntity:
          Name:
            NameFull: Subramaniam, Sreeramanan
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 06
              Text: Jun2022
              Type: published
              Y: 2022
          Identifiers:
            – Type: issn-print
              Value: 18635466
          Numbering:
            – Type: volume
              Value: 16
            – Type: issue
              Value: 3
          Titles:
            – TitleFull: Plant Biotechnology Reports
              Type: main
ResultId 1