Cytological heterozygosity and the hybrid origin of sweet orange [Citrus sinensis (L.) Osbeck].
Saved in:
| Title: | Cytological heterozygosity and the hybrid origin of sweet orange [Citrus sinensis (L.) Osbeck]. |
|---|---|
| Authors: | Pedrosa, A.1 apedrosa@s1.botanik.univie.ac.at, Schweizer, D.1, Guerra, M.1 |
| Source: | Theoretical & Applied Genetics. Feb2000, Vol. 100 Issue 3/4, p361-367. 7p. |
| Subjects: | Cytology, Citrus, Chromosomes, RNA, In situ hybridization, Genetics |
| Abstract: | Citrus sinensis chromosomes, although small in size, present a remarkable differentiation of bands with the fluorochromes CMA and DAPI. These bands suggest that some heteromorphisms are fixed in this species. To investigate the extension of these heteromorphisms, ten cultivars of C. sinenesis were analysed with CMA/DAPI staining and, in some of them, the 18S–5.8S–25S rRNA and 5S rRNA genes were located by in situ hybridization. CMA/DAPI staining showed exactly the same CMA+/DAPI- banding pattern for all cultivars. In situ hybridization revealed three 18S–5.8S–25S rRNA gene sites, two proximally located on two similar chromosomes and one terminally located on a third non-related chromosome. Two 5S rRNA gene sites were observed in this species, with one located proximal to the telomeric 18S–5.8S–25S rDNA site. Both cytological approaches revealed an invariable, heterozygotic karyotype among sweet orange cultivars. Based on these data, the putative hybrid origin of the species is discussed. [ABSTRACT FROM AUTHOR] |
| Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 15737158 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Cytological heterozygosity and the hybrid origin of sweet orange [Citrus sinensis (L.) Osbeck]. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Pedrosa%2C+A%2E%22">Pedrosa, A.</searchLink><relatesTo>1</relatesTo><i> apedrosa@s1.botanik.univie.ac.at</i><br /><searchLink fieldCode="AR" term="%22Schweizer%2C+D%2E%22">Schweizer, D.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Guerra%2C+M%2E%22">Guerra, M.</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. Feb2000, Vol. 100 Issue 3/4, p361-367. 7p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Cytology%22">Cytology</searchLink><br /><searchLink fieldCode="DE" term="%22Citrus%22">Citrus</searchLink><br /><searchLink fieldCode="DE" term="%22Chromosomes%22">Chromosomes</searchLink><br /><searchLink fieldCode="DE" term="%22RNA%22">RNA</searchLink><br /><searchLink fieldCode="DE" term="%22In+situ+hybridization%22">In situ hybridization</searchLink><br /><searchLink fieldCode="DE" term="%22Genetics%22">Genetics</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Citrus sinensis chromosomes, although small in size, present a remarkable differentiation of bands with the fluorochromes CMA and DAPI. These bands suggest that some heteromorphisms are fixed in this species. To investigate the extension of these heteromorphisms, ten cultivars of C. sinenesis were analysed with CMA/DAPI staining and, in some of them, the 18S–5.8S–25S rRNA and 5S rRNA genes were located by in situ hybridization. CMA/DAPI staining showed exactly the same CMA+/DAPI- banding pattern for all cultivars. In situ hybridization revealed three 18S–5.8S–25S rRNA gene sites, two proximally located on two similar chromosomes and one terminally located on a third non-related chromosome. Two 5S rRNA gene sites were observed in this species, with one located proximal to the telomeric 18S–5.8S–25S rDNA site. Both cytological approaches revealed an invariable, heterozygotic karyotype among sweet orange cultivars. Based on these data, the putative hybrid origin of the species is discussed. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=15737158 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s001220050047 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 361 Subjects: – SubjectFull: Cytology Type: general – SubjectFull: Citrus Type: general – SubjectFull: Chromosomes Type: general – SubjectFull: RNA Type: general – SubjectFull: In situ hybridization Type: general – SubjectFull: Genetics Type: general Titles: – TitleFull: Cytological heterozygosity and the hybrid origin of sweet orange [Citrus sinensis (L.) Osbeck]. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Pedrosa, A. – PersonEntity: Name: NameFull: Schweizer, D. – PersonEntity: Name: NameFull: Guerra, M. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 02 Text: Feb2000 Type: published Y: 2000 Identifiers: – Type: issn-print Value: 00405752 Numbering: – Type: volume Value: 100 – Type: issue Value: 3/4 Titles: – TitleFull: Theoretical & Applied Genetics Type: main |
| ResultId | 1 |
