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Titanium alkalinization improves response of osteoblasts to zoledronic acid.

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Title: Titanium alkalinization improves response of osteoblasts to zoledronic acid.
Authors: Vila Real, Rodrigo Paes1 (AUTHOR), Pansani, Taisa Nogueira2 (AUTHOR), Cardoso, Lais Medeiros3 (AUTHOR), de Souza Costa, Carlos Alberto2 (AUTHOR), Basso, Fernanda Gonçalves1 (AUTHOR) fergbasso@gmail.com
Source: Biointerphases. May2022, Vol. 17 Issue 3, p1-8. 8p.
Subjects: Zoledronic acid, Alkalinization, Osteoblasts, X-ray photoelectron spectroscopy, Titanium, Two-way analysis of variance, Cell survival, Bone growth
Abstract: This investigation is aimed to determine the effect of the modification of titanium surface with NaOH on the metabolism of osteoblasts treated with zoledronic acid (ZA). Machined and NaOH-treated titanium disks were used. Surfaces were characterized by scanning electron microscopy, confocal microscopy, and x-ray photoelectron spectroscopy (XPS) analysis. Human osteoblasts were seeded onto the disks. After 24 h, cells were treated with ZA at 5 μM for 7 days. At this point, cell viability, collagen synthesis, total protein production, alkaline phosphatase activity, and mineral nodule deposition were assessed. The results of surface roughness were descriptively and statistically analyzed (t-Student), while the XPS results were qualitatively described. Cell metabolism data were analyzed by the analysis of variance two-way and Tukey tests at a 5% significance level. The results demonstrated that NaOH-treatment increased surface roughness (p <.05) and confirmed the presence of sodium titanate and a pH switch on the NaOH-treated disks. This modification also resulted in higher cell viability, collagen synthesis, total protein production, and alkaline phosphatase by osteoblasts when compared to cells seeded onto machined disks (p < 0.05). In the presence of ZA, all cellular metabolism and differentiation parameters were significantly reduced for cells seeded on both surfaces (p < 0.05); however, the cells seeded onto modified surfaces showed higher values for these parameters, except for mineral nodule deposition (p < 0.05). NaOH modification improved cell adhesion and metabolism of osteogenic cells even in the presence of ZA. The surface modification of titanium with NaOH solution may be an interesting strategy to improve metabolism and differentiation of osteoblasts and accelerate osseointegration process, mainly for tissues exposed to ZA. [ABSTRACT FROM AUTHOR]
Copyright of Biointerphases is the property of American Institute of Physics and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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  Data: Titanium alkalinization improves response of osteoblasts to zoledronic acid.
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  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Vila+Real%2C+Rodrigo+Paes%22&quot;&gt;Vila Real, Rodrigo Paes&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Pansani%2C+Taisa+Nogueira%22&quot;&gt;Pansani, Taisa Nogueira&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Cardoso%2C+Lais+Medeiros%22&quot;&gt;Cardoso, Lais Medeiros&lt;/searchLink&gt;&lt;relatesTo&gt;3&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22de+Souza+Costa%2C+Carlos+Alberto%22&quot;&gt;de Souza Costa, Carlos Alberto&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Basso%2C+Fernanda+Gon&#231;alves%22&quot;&gt;Basso, Fernanda Gon&#231;alves&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;i&gt; fergbasso@gmail.com&lt;/i&gt;
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  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%22Biointerphases%22&quot;&gt;Biointerphases&lt;/searchLink&gt;. May2022, Vol. 17 Issue 3, p1-8. 8p.
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  Data: &lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Zoledronic+acid%22&quot;&gt;Zoledronic acid&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Alkalinization%22&quot;&gt;Alkalinization&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Osteoblasts%22&quot;&gt;Osteoblasts&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22X-ray+photoelectron+spectroscopy%22&quot;&gt;X-ray photoelectron spectroscopy&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Titanium%22&quot;&gt;Titanium&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Two-way+analysis+of+variance%22&quot;&gt;Two-way analysis of variance&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Cell+survival%22&quot;&gt;Cell survival&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Bone+growth%22&quot;&gt;Bone growth&lt;/searchLink&gt;
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: This investigation is aimed to determine the effect of the modification of titanium surface with NaOH on the metabolism of osteoblasts treated with zoledronic acid (ZA). Machined and NaOH-treated titanium disks were used. Surfaces were characterized by scanning electron microscopy, confocal microscopy, and x-ray photoelectron spectroscopy (XPS) analysis. Human osteoblasts were seeded onto the disks. After 24 h, cells were treated with ZA at 5 μM for 7 days. At this point, cell viability, collagen synthesis, total protein production, alkaline phosphatase activity, and mineral nodule deposition were assessed. The results of surface roughness were descriptively and statistically analyzed (t-Student), while the XPS results were qualitatively described. Cell metabolism data were analyzed by the analysis of variance two-way and Tukey tests at a 5% significance level. The results demonstrated that NaOH-treatment increased surface roughness (p &lt;.05) and confirmed the presence of sodium titanate and a pH switch on the NaOH-treated disks. This modification also resulted in higher cell viability, collagen synthesis, total protein production, and alkaline phosphatase by osteoblasts when compared to cells seeded onto machined disks (p &lt; 0.05). In the presence of ZA, all cellular metabolism and differentiation parameters were significantly reduced for cells seeded on both surfaces (p &lt; 0.05); however, the cells seeded onto modified surfaces showed higher values for these parameters, except for mineral nodule deposition (p &lt; 0.05). NaOH modification improved cell adhesion and metabolism of osteogenic cells even in the presence of ZA. The surface modification of titanium with NaOH solution may be an interesting strategy to improve metabolism and differentiation of osteoblasts and accelerate osseointegration process, mainly for tissues exposed to ZA. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Group: Ab
  Data: &lt;i&gt;Copyright of Biointerphases is the property of American Institute of Physics and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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    Identifiers:
      – Type: doi
        Value: 10.1116/6.0001670
    Languages:
      – Code: eng
        Text: English
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      Pagination:
        PageCount: 8
        StartPage: 1
    Subjects:
      – SubjectFull: Zoledronic acid
        Type: general
      – SubjectFull: Alkalinization
        Type: general
      – SubjectFull: Osteoblasts
        Type: general
      – SubjectFull: X-ray photoelectron spectroscopy
        Type: general
      – SubjectFull: Titanium
        Type: general
      – SubjectFull: Two-way analysis of variance
        Type: general
      – SubjectFull: Cell survival
        Type: general
      – SubjectFull: Bone growth
        Type: general
    Titles:
      – TitleFull: Titanium alkalinization improves response of osteoblasts to zoledronic acid.
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            NameFull: Vila Real, Rodrigo Paes
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            NameFull: Pansani, Taisa Nogueira
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            NameFull: Cardoso, Lais Medeiros
      – PersonEntity:
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            NameFull: de Souza Costa, Carlos Alberto
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            NameFull: Basso, Fernanda Gonçalves
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            – D: 01
              M: 05
              Text: May2022
              Type: published
              Y: 2022
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              Value: 17
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