View in EDS

Obtaining a freeze-dried biomaterial for skin regeneration: Reinforcement of the microstructure through the use of crosslinkers and in vivo application.

Saved in:
Bibliographic Details
Title: Obtaining a freeze-dried biomaterial for skin regeneration: Reinforcement of the microstructure through the use of crosslinkers and in vivo application.
Authors: Mello Zamudio, Raphaela1 (AUTHOR), Mattos Pereira, Vitoria2 (AUTHOR), López Angulo, Daniel Enrique1,3,4 (AUTHOR) daniel.lopez@usp.br, Ambrosio, Carlos Eduardo2 (AUTHOR), Lourenço, Rodrigo Vinicius3 (AUTHOR), Martins, Daniele dos Santos2 (AUTHOR), Akashi, Larissa1 (AUTHOR), Bittante, Ana Mônica Q.B.3 (AUTHOR), do Amaral Sobral, Paulo José3,4 (AUTHOR)
Source: Materials Chemistry & Physics. Oct2022, Vol. 290, pN.PAG-N.PAG. 1p.
Subjects: Skin regeneration, Aloe vera, Biomaterials, Wound healing, Microstructure, Glutaraldehyde, Pore size distribution
Abstract: Scaffolds are one of the emerging alternatives to the traditional techniques of tissue and organ repair and regeneration. They offer the necessary support to the healthy formation of new tissues. Highly porous and interconnected scaffolds for skin regeneration were manufactured and studied with two types of crosslinking, genipin and glutaraldehyde. Two accessible biopolymers, chitosan and gelatin, were used together as matrix and were incorporated with snail mucus and aloe vera like additives. The microstructure and thermal properties analysis determined that the best crosslinker was glutaraldehyde, presenting a more homogeneous range of pore sizes, uniform distribution of the porous structure and higher denaturation temperatures showing a greater degree of crosslinking and stabilization. Thus, in vivo studies were conducted with the implantation of these scaffolds in mice after causing a burn wound. The addition of Aloe vera in the scaffolds is highlighted, apparently the large number of glycoproteins present in Aloe vera were able to integrate well into the scaffolds. Their diffusion towards the damaged tissue allowed accelerated healing and injury reduction. The material made with aloe vera crosslinked with glutaraldehyde showed promising results in accelerating the healing process in vivo studies, suggesting a great skin substitute for wound healing applications. • Glutaraldehyde crosslinker enabled a uniform microstructure and stable properties. • Incorporating aloe vera and snail mucus increased the G-C scaffold porosity. • G- C scaffolds crosslinked with glutaraldehyde reduced the wound size faster. • G- C scaffolds with Aloe vera also showed great results in wound healing. [ABSTRACT FROM AUTHOR]
Copyright of Materials Chemistry & Physics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Description
Abstract:Scaffolds are one of the emerging alternatives to the traditional techniques of tissue and organ repair and regeneration. They offer the necessary support to the healthy formation of new tissues. Highly porous and interconnected scaffolds for skin regeneration were manufactured and studied with two types of crosslinking, genipin and glutaraldehyde. Two accessible biopolymers, chitosan and gelatin, were used together as matrix and were incorporated with snail mucus and aloe vera like additives. The microstructure and thermal properties analysis determined that the best crosslinker was glutaraldehyde, presenting a more homogeneous range of pore sizes, uniform distribution of the porous structure and higher denaturation temperatures showing a greater degree of crosslinking and stabilization. Thus, in vivo studies were conducted with the implantation of these scaffolds in mice after causing a burn wound. The addition of Aloe vera in the scaffolds is highlighted, apparently the large number of glycoproteins present in Aloe vera were able to integrate well into the scaffolds. Their diffusion towards the damaged tissue allowed accelerated healing and injury reduction. The material made with aloe vera crosslinked with glutaraldehyde showed promising results in accelerating the healing process in vivo studies, suggesting a great skin substitute for wound healing applications. • Glutaraldehyde crosslinker enabled a uniform microstructure and stable properties. • Incorporating aloe vera and snail mucus increased the G-C scaffold porosity. • G- C scaffolds crosslinked with glutaraldehyde reduced the wound size faster. • G- C scaffolds with Aloe vera also showed great results in wound healing. [ABSTRACT FROM AUTHOR]
ISSN:02540584
DOI:10.1016/j.matchemphys.2022.126544