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Distinct Uptake Routes Participate in Silver Nanoparticle Engulfment by Earthworm and Human Immune Cells.

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Title: Distinct Uptake Routes Participate in Silver Nanoparticle Engulfment by Earthworm and Human Immune Cells.
Authors: Kokhanyuk, Bohdana1 (AUTHOR), Vántus, Viola Bagóné2 (AUTHOR), Radnai, Balázs2 (AUTHOR), Vámos, Eszter2 (AUTHOR), Kajner, Gyula3 (AUTHOR), Galbács, Gábor3 (AUTHOR), Telek, Elek4 (AUTHOR), Mészáros, Mária5 (AUTHOR), Deli, Mária A.5 (AUTHOR), Németh, Péter1 (AUTHOR), Engelmann, Péter1 (AUTHOR) engelmann.peter@pte.hu
Source: Nanomaterials (2079-4991). Aug2022, Vol. 12 Issue 16, p2818-2818. 20p.
Subjects: Earthworms, Nanomedicine, Pinocytosis, Endocytosis, Silver, Eisenia, Phagocytosis, Microtubules
Abstract: The consequences of engineered silver nanoparticle (AgNP) exposure and cellular interaction with the immune system are poorly understood. The immunocytes of the Eisenia andrei earthworm are frequently applied in ecotoxicological studies and possess functional similarity to vertebrate macrophages. Hence, we characterized and compared the endocytosis mechanisms for the uptake of 75 nm AgNPs by earthworm coelomocytes, human THP-1 monocytes, and differentiated THP-1 (macrophage-like) cells. Our results indicate that microtubule-dependent, scavenger–receptor, and PI3K signaling-mediated macropinocytosis are utilized during AgNP engulfment by human THP-1 and differentiated THP-1 cells. However, earthworm coelomocytes employ actin-dependent phagocytosis during AgNPs uptake. In both human and earthworm immunocytes, AgNPs were located in the cytoplasm, within the endo-/lysosomes. We detected that the internalization of AgNPs is TLR/MyD88-dependent, also involving the bactericidal/permeability-increasing protein (BPI) in the case of human immunocytes. The exposure led to decreased mitochondrial respiration in human immunocytes; however, in coelomocytes, it enhanced respiratory parameters. Our findings provide more data about NP trafficking as nano-carriers in the nanomedicine field, as well as contribute to an understanding of the ecotoxicological consequences of nanoparticle exposure. [ABSTRACT FROM AUTHOR]
Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Distinct Uptake Routes Participate in Silver Nanoparticle Engulfment by Earthworm and Human Immune Cells.
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  Data: <searchLink fieldCode="AR" term="%22Kokhanyuk%2C+Bohdana%22">Kokhanyuk, Bohdana</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Vántus%2C+Viola+Bagóné%22">Vántus, Viola Bagóné</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Radnai%2C+Balázs%22">Radnai, Balázs</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Vámos%2C+Eszter%22">Vámos, Eszter</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kajner%2C+Gyula%22">Kajner, Gyula</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Galbács%2C+Gábor%22">Galbács, Gábor</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Telek%2C+Elek%22">Telek, Elek</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mészáros%2C+Mária%22">Mészáros, Mária</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Deli%2C+Mária+A%2E%22">Deli, Mária A.</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Németh%2C+Péter%22">Németh, Péter</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Engelmann%2C+Péter%22">Engelmann, Péter</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> engelmann.peter@pte.hu</i>
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  Data: <searchLink fieldCode="JN" term="%22Nanomaterials+%282079-4991%29%22">Nanomaterials (2079-4991)</searchLink>. Aug2022, Vol. 12 Issue 16, p2818-2818. 20p.
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  Data: <searchLink fieldCode="DE" term="%22Earthworms%22">Earthworms</searchLink><br /><searchLink fieldCode="DE" term="%22Nanomedicine%22">Nanomedicine</searchLink><br /><searchLink fieldCode="DE" term="%22Pinocytosis%22">Pinocytosis</searchLink><br /><searchLink fieldCode="DE" term="%22Endocytosis%22">Endocytosis</searchLink><br /><searchLink fieldCode="DE" term="%22Silver%22">Silver</searchLink><br /><searchLink fieldCode="DE" term="%22Eisenia%22">Eisenia</searchLink><br /><searchLink fieldCode="DE" term="%22Phagocytosis%22">Phagocytosis</searchLink><br /><searchLink fieldCode="DE" term="%22Microtubules%22">Microtubules</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The consequences of engineered silver nanoparticle (AgNP) exposure and cellular interaction with the immune system are poorly understood. The immunocytes of the Eisenia andrei earthworm are frequently applied in ecotoxicological studies and possess functional similarity to vertebrate macrophages. Hence, we characterized and compared the endocytosis mechanisms for the uptake of 75 nm AgNPs by earthworm coelomocytes, human THP-1 monocytes, and differentiated THP-1 (macrophage-like) cells. Our results indicate that microtubule-dependent, scavenger–receptor, and PI3K signaling-mediated macropinocytosis are utilized during AgNP engulfment by human THP-1 and differentiated THP-1 cells. However, earthworm coelomocytes employ actin-dependent phagocytosis during AgNPs uptake. In both human and earthworm immunocytes, AgNPs were located in the cytoplasm, within the endo-/lysosomes. We detected that the internalization of AgNPs is TLR/MyD88-dependent, also involving the bactericidal/permeability-increasing protein (BPI) in the case of human immunocytes. The exposure led to decreased mitochondrial respiration in human immunocytes; however, in coelomocytes, it enhanced respiratory parameters. Our findings provide more data about NP trafficking as nano-carriers in the nanomedicine field, as well as contribute to an understanding of the ecotoxicological consequences of nanoparticle exposure. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.3390/nano12162818
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      – Code: eng
        Text: English
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        PageCount: 20
        StartPage: 2818
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      – SubjectFull: Earthworms
        Type: general
      – SubjectFull: Nanomedicine
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      – SubjectFull: Pinocytosis
        Type: general
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      – SubjectFull: Silver
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      – SubjectFull: Phagocytosis
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      – SubjectFull: Microtubules
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              Text: Aug2022
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