View in EDS

Isolation and characterization of novel soil bacterium, Klebsiella pneumoniae strain GS7-1 for the degradation of zearalenone in major cereals.

Saved in:
Bibliographic Details
Title: Isolation and characterization of novel soil bacterium, Klebsiella pneumoniae strain GS7-1 for the degradation of zearalenone in major cereals.
Authors: Imade, Francis Nosakhare1,2 (AUTHOR), Humza, Muhammad1 (AUTHOR), Dada, Oyeyemi Adigun3 (AUTHOR), Ullah, Sana1 (AUTHOR), Jahan, Israt1 (AUTHOR), Eseigbe, Daniel2 (AUTHOR), Geng, Hairong1 (AUTHOR), Zheng, Yongquan4 (AUTHOR), Xing, Fuguo1 (AUTHOR) xingfuguo@caas.cn, Liu, Yang1,5 (AUTHOR) liuyang@fosu.edu.cn
Source: Food Control. Jan2023, Vol. 143, pN.PAG-N.PAG. 1p.
Subjects: Klebsiella pneumoniae, Soil microbiology, Chemical purification, Zearalenone, Bacterial cells
Geographic Terms: Shandong Sheng (China), China
Abstract: Zearalenone (ZEN) is one of the major agricultural feed contaminants causing serious health hazards worldwide and its degradation by beneficial microbial agents is now being widely examined. In this study we investigated the degradation ability of Klebsiella pneumoniae strain GS7-1 isolated from corn field in Shandong province in China on ZEN. The effects of temperature, incubation time and pH were studied. Furthermore, it was examined if the washed cell pellet and the supernatant of fermented culture (SFC) of the bacterial cell components could detoxify ZEN. ZEN removal increased with increase in pH from 61.3% at pH 5–100% at pH 8. The minimum and maximum temperatures at which K. pneumoniae strain GS7-1 degraded ZEN were 28 °C and 60 °C, respectively. Increase in incubation time increased the degradation effect as ZEN was completely degraded at 96 h. The SFC of strain GS7-1 reduced the ZEN content by 100%, while the first and second washed of the cell pellets in PBS reduced the ZEN content by 30% and 25%, respectively. The rate of SFC degradation significantly decreased after treatment with proteinase K, SDS and the combination of proteinase K and SDS. There exists a significant correlation between temperature, incubation time and pH regarding ZEN degradation by strain GS7-1. The results also suggest that enzymes present in the supernatant of Klebsiella pneumoniae GS7-1 were involved in ZEN degradation. The biodegradation of ZEN is a very feasible method for food and feed purification as physical and chemical methods may not only eliminate mycotoxins, but also nutrients and sensory-related compounds. • A novel Zearalenone degrading bacterium was isolated and identified as Klebsiella pneumonia strain GS 7-1. • The degradation of ZEN by K pneumonia strain GS 7–1 IS pH and time dependent. • This is the first report of that K. pneumoniae degrading ZEN to date. [ABSTRACT FROM AUTHOR]
Copyright of Food Control is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 159056975
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Isolation and characterization of novel soil bacterium, Klebsiella pneumoniae strain GS7-1 for the degradation of zearalenone in major cereals.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Imade%2C+Francis+Nosakhare%22">Imade, Francis Nosakhare</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Humza%2C+Muhammad%22">Humza, Muhammad</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Dada%2C+Oyeyemi+Adigun%22">Dada, Oyeyemi Adigun</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ullah%2C+Sana%22">Ullah, Sana</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jahan%2C+Israt%22">Jahan, Israt</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Eseigbe%2C+Daniel%22">Eseigbe, Daniel</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Geng%2C+Hairong%22">Geng, Hairong</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zheng%2C+Yongquan%22">Zheng, Yongquan</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xing%2C+Fuguo%22">Xing, Fuguo</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> xingfuguo@caas.cn</i><br /><searchLink fieldCode="AR" term="%22Liu%2C+Yang%22">Liu, Yang</searchLink><relatesTo>1,5</relatesTo> (AUTHOR)<i> liuyang@fosu.edu.cn</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Food+Control%22">Food Control</searchLink>. Jan2023, Vol. 143, pN.PAG-N.PAG. 1p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Klebsiella+pneumoniae%22">Klebsiella pneumoniae</searchLink><br /><searchLink fieldCode="DE" term="%22Soil+microbiology%22">Soil microbiology</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+purification%22">Chemical purification</searchLink><br /><searchLink fieldCode="DE" term="%22Zearalenone%22">Zearalenone</searchLink><br /><searchLink fieldCode="DE" term="%22Bacterial+cells%22">Bacterial cells</searchLink>
– Name: SubjectGeographic
  Label: Geographic Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Shandong+Sheng+%28China%29%22">Shandong Sheng (China)</searchLink><br /><searchLink fieldCode="DE" term="%22China%22">China</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Zearalenone (ZEN) is one of the major agricultural feed contaminants causing serious health hazards worldwide and its degradation by beneficial microbial agents is now being widely examined. In this study we investigated the degradation ability of Klebsiella pneumoniae strain GS7-1 isolated from corn field in Shandong province in China on ZEN. The effects of temperature, incubation time and pH were studied. Furthermore, it was examined if the washed cell pellet and the supernatant of fermented culture (SFC) of the bacterial cell components could detoxify ZEN. ZEN removal increased with increase in pH from 61.3% at pH 5–100% at pH 8. The minimum and maximum temperatures at which K. pneumoniae strain GS7-1 degraded ZEN were 28 °C and 60 °C, respectively. Increase in incubation time increased the degradation effect as ZEN was completely degraded at 96 h. The SFC of strain GS7-1 reduced the ZEN content by 100%, while the first and second washed of the cell pellets in PBS reduced the ZEN content by 30% and 25%, respectively. The rate of SFC degradation significantly decreased after treatment with proteinase K, SDS and the combination of proteinase K and SDS. There exists a significant correlation between temperature, incubation time and pH regarding ZEN degradation by strain GS7-1. The results also suggest that enzymes present in the supernatant of Klebsiella pneumoniae GS7-1 were involved in ZEN degradation. The biodegradation of ZEN is a very feasible method for food and feed purification as physical and chemical methods may not only eliminate mycotoxins, but also nutrients and sensory-related compounds. • A novel Zearalenone degrading bacterium was isolated and identified as Klebsiella pneumonia strain GS 7-1. • The degradation of ZEN by K pneumonia strain GS 7–1 IS pH and time dependent. • This is the first report of that K. pneumoniae degrading ZEN to date. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Food Control is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=159056975
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.foodcont.2022.109287
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Klebsiella pneumoniae
        Type: general
      – SubjectFull: Soil microbiology
        Type: general
      – SubjectFull: Chemical purification
        Type: general
      – SubjectFull: Zearalenone
        Type: general
      – SubjectFull: Bacterial cells
        Type: general
      – SubjectFull: Shandong Sheng (China)
        Type: general
      – SubjectFull: China
        Type: general
    Titles:
      – TitleFull: Isolation and characterization of novel soil bacterium, Klebsiella pneumoniae strain GS7-1 for the degradation of zearalenone in major cereals.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Imade, Francis Nosakhare
      – PersonEntity:
          Name:
            NameFull: Humza, Muhammad
      – PersonEntity:
          Name:
            NameFull: Dada, Oyeyemi Adigun
      – PersonEntity:
          Name:
            NameFull: Ullah, Sana
      – PersonEntity:
          Name:
            NameFull: Jahan, Israt
      – PersonEntity:
          Name:
            NameFull: Eseigbe, Daniel
      – PersonEntity:
          Name:
            NameFull: Geng, Hairong
      – PersonEntity:
          Name:
            NameFull: Zheng, Yongquan
      – PersonEntity:
          Name:
            NameFull: Xing, Fuguo
      – PersonEntity:
          Name:
            NameFull: Liu, Yang
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 01
              Text: Jan2023
              Type: published
              Y: 2023
          Identifiers:
            – Type: issn-print
              Value: 09567135
          Numbering:
            – Type: volume
              Value: 143
          Titles:
            – TitleFull: Food Control
              Type: main
ResultId 1