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Fine mapping of the reduced height gene Rht22 in tetraploid wheat landrace Jianyangailanmai (Triticum turgidum L.).

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Title: Fine mapping of the reduced height gene Rht22 in tetraploid wheat landrace Jianyangailanmai (Triticum turgidum L.).
Authors: Wang, Chao1 (AUTHOR), Bao, Yunjing1 (AUTHOR), Yao, Qin1 (AUTHOR), Long, Dan1 (AUTHOR), Xiao, Xue1 (AUTHOR), Fan, Xing1 (AUTHOR), Kang, Houyang1 (AUTHOR), Zeng, Jian2 (AUTHOR), Sha, Lina1 (AUTHOR), Zhang, Haiqin1 (AUTHOR), Wu, Dandan1 (AUTHOR), Zhou, Yonghong1 (AUTHOR), Zhou, Qiang3 (AUTHOR) zhouqiang@cib.ac.cn, Wang, Yi1 (AUTHOR) wangyi@sicau.edu.cn, Cheng, Yiran1 (AUTHOR) chengyiran@sicau.edu.cn
Source: Theoretical & Applied Genetics. Oct2022, Vol. 135 Issue 10, p3643-3660. 18p.
Subjects: Emmer wheat, Hormone synthesis, Wheat, DNA replication, Germplasm, Durum wheat, Gene mapping, Plant gene mapping
Abstract: Key message: Rht22 was fine mapped in the interval of 0.53–1.48 Mb on 7AS, which reduces cell number of internode to cause semi-dwarfism in Jianyangailanmai. As a valuable germplasm resource for wheat genetic improvement, tetraploid wheat has several reduced height (Rht) and enhanced harvest index genes. Rht22, discovered in Jianyangailanmai (JAM, Triticum turgidum L., 2n = 4x = 28, AABB), significantly increases the spikelet number per spike, but its accurate chromosomal position is still unknown. In this study, a high-density genetic map was constructed using specific-length amplified fragment sequencing in an F7 RIL_DJ population, which was derived from a cross between dwarf Polish wheat (T. polonicum L., 2n = 4x = 28, AABB) and JAM. Two plant height loci, Qph.sicau-4B and Qph.sicau-7A, were mapped on chromosomes 4BS and 7AS, respectively. Qph.sicau-7A was mapped to the 0.33-4.46 Mb interval on 7AS and likely represents the candidate region of Rht22. Fine mapping confirmed and narrowed Rht22 on chromosome arm 7AS between Xbag295.s53 and Xb295.191 in three different populations. The physical region ranged from 0.53 to 1.48 Mb and included 18 candidate genes. Transcriptome analysis of two pairs of near-isogenic lines revealed that 135 differentially expressed genes (DEGs) were associated with semi-dwarfism. Of these, the expression of 83 annotated DEGs involved in hormones synthesis and signal transduction, cell wall composition, DNA replication, microtubule and phragmoplast arrays was significantly down-regulated in the semi-dwarf line. Therefore, Rht22 causes semi-dwarfism in JAM by disrupting these cellular processes, which impairs cell proliferation and reduces internode cell number. [ABSTRACT FROM AUTHOR]
Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Label: Title
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  Data: Fine mapping of the reduced height gene Rht22 in tetraploid wheat landrace Jianyangailanmai (Triticum turgidum L.).
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  Data: <searchLink fieldCode="AR" term="%22Wang%2C+Chao%22">Wang, Chao</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bao%2C+Yunjing%22">Bao, Yunjing</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yao%2C+Qin%22">Yao, Qin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Long%2C+Dan%22">Long, Dan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xiao%2C+Xue%22">Xiao, Xue</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Fan%2C+Xing%22">Fan, Xing</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kang%2C+Houyang%22">Kang, Houyang</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zeng%2C+Jian%22">Zeng, Jian</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sha%2C+Lina%22">Sha, Lina</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Haiqin%22">Zhang, Haiqin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wu%2C+Dandan%22">Wu, Dandan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhou%2C+Yonghong%22">Zhou, Yonghong</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhou%2C+Qiang%22">Zhou, Qiang</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> zhouqiang@cib.ac.cn</i><br /><searchLink fieldCode="AR" term="%22Wang%2C+Yi%22">Wang, Yi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> wangyi@sicau.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Cheng%2C+Yiran%22">Cheng, Yiran</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> chengyiran@sicau.edu.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. Oct2022, Vol. 135 Issue 10, p3643-3660. 18p.
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  Data: <searchLink fieldCode="DE" term="%22Emmer+wheat%22">Emmer wheat</searchLink><br /><searchLink fieldCode="DE" term="%22Hormone+synthesis%22">Hormone synthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Wheat%22">Wheat</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+replication%22">DNA replication</searchLink><br /><searchLink fieldCode="DE" term="%22Germplasm%22">Germplasm</searchLink><br /><searchLink fieldCode="DE" term="%22Durum+wheat%22">Durum wheat</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+mapping%22">Gene mapping</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+gene+mapping%22">Plant gene mapping</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Key message: Rht22 was fine mapped in the interval of 0.53–1.48 Mb on 7AS, which reduces cell number of internode to cause semi-dwarfism in Jianyangailanmai. As a valuable germplasm resource for wheat genetic improvement, tetraploid wheat has several reduced height (Rht) and enhanced harvest index genes. Rht22, discovered in Jianyangailanmai (JAM, Triticum turgidum L., 2n = 4x = 28, AABB), significantly increases the spikelet number per spike, but its accurate chromosomal position is still unknown. In this study, a high-density genetic map was constructed using specific-length amplified fragment sequencing in an F7 RIL_DJ population, which was derived from a cross between dwarf Polish wheat (T. polonicum L., 2n = 4x = 28, AABB) and JAM. Two plant height loci, Qph.sicau-4B and Qph.sicau-7A, were mapped on chromosomes 4BS and 7AS, respectively. Qph.sicau-7A was mapped to the 0.33-4.46 Mb interval on 7AS and likely represents the candidate region of Rht22. Fine mapping confirmed and narrowed Rht22 on chromosome arm 7AS between Xbag295.s53 and Xb295.191 in three different populations. The physical region ranged from 0.53 to 1.48 Mb and included 18 candidate genes. Transcriptome analysis of two pairs of near-isogenic lines revealed that 135 differentially expressed genes (DEGs) were associated with semi-dwarfism. Of these, the expression of 83 annotated DEGs involved in hormones synthesis and signal transduction, cell wall composition, DNA replication, microtubule and phragmoplast arrays was significantly down-regulated in the semi-dwarf line. Therefore, Rht22 causes semi-dwarfism in JAM by disrupting these cellular processes, which impairs cell proliferation and reduces internode cell number. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1007/s00122-022-04207-8
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      – Code: eng
        Text: English
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        PageCount: 18
        StartPage: 3643
    Subjects:
      – SubjectFull: Emmer wheat
        Type: general
      – SubjectFull: Hormone synthesis
        Type: general
      – SubjectFull: Wheat
        Type: general
      – SubjectFull: DNA replication
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      – SubjectFull: Germplasm
        Type: general
      – SubjectFull: Durum wheat
        Type: general
      – SubjectFull: Gene mapping
        Type: general
      – SubjectFull: Plant gene mapping
        Type: general
    Titles:
      – TitleFull: Fine mapping of the reduced height gene Rht22 in tetraploid wheat landrace Jianyangailanmai (Triticum turgidum L.).
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              Text: Oct2022
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