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Analysis of two intestinal bacterial metabolites (trimethylamine N-oxide and phenylacetylglutamine) in human serum samples of patients with T2DM and AMI using a liquid chromatography tandem mass spectrometry method.

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Title: Analysis of two intestinal bacterial metabolites (trimethylamine N-oxide and phenylacetylglutamine) in human serum samples of patients with T2DM and AMI using a liquid chromatography tandem mass spectrometry method.
Authors: Tang, Yueming1 (AUTHOR), Zou, Yutong1 (AUTHOR), Cui, Jingwen2 (AUTHOR), Ma, Xiaoli1,3 (AUTHOR), Zhang, Li4 (AUTHOR), Yu, Songlin1 (AUTHOR) yusonglinpku@163.com, Qiu, Ling1,5 (AUTHOR) lingqiubj@163.com
Source: Clinica Chimica Acta. Nov2022, Vol. 536, p162-168. 7p.
Subjects: Liquid chromatography-mass spectrometry, Bacterial metabolites, Intestinal mucosa, Type 2 diabetes, Trimethylamine, Myocardial infarction
Abstract: Trimethylamine N -oxide (TMAO) and phenylacetylglutamine (PAGln) are associated with acute myocardial infarction (AMI) and type 2 diabetes mellitus (T2DM). This study developed and validated a simple method firstly for simultaneously quantifying serum TMAO and PAGln using liquid chromatography-tandem mass spectrometry (LC–MS/MS). Serum samples from patients with T2DM, AMI, and healthy subjects were analyzed using a new LC–MS/MS method to evaluate TMAO and PAGln levels. Statistical analyses were performed to evaluate TMAO and PAGln distributions among different groups. Retention and separation of the two metabolites were achieved within 5 min. For both analytes, the assay was linear in a 0.02–10 µg/mL range, with >0.99 average linear correlation coefficients, and quantification limit values of approximately 0.010 µg/mL. The average recoveries of TMAO and PAGln were 96.3 % and 96.4 %, respectively. The intra-run and total coefficient variations were 3.5–4.8 % and 3.9–5.7 % respectively for TMAO; and 4.0–5.1 % and 4.6–6.3 % respectively for PAGln. TMAO and PAGln showed a moderate correlation (P < 0.001) and their levels in patients with T2DM were significantly higher than those in healthy individuals (P < 0.001). TMAO levels were higher in patients with T2DM than in patients with AMI (P < 0.01). Patients with AMI had higher PAGln levels than healthy individuals (P < 0.05). After adjusting for sex and age, the top tertile of PAGln was positively correlated with T2DM and AMI while that of TMAO was positively correlated with T2DM. Overall, a robust isotope dilution LC–MS/MS method was established, which may be beneficial for assessing the association between two metabolites with AMI and T2DM. [ABSTRACT FROM AUTHOR]
Copyright of Clinica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
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  Data: Analysis of two intestinal bacterial metabolites (trimethylamine N-oxide and phenylacetylglutamine) in human serum samples of patients with T2DM and AMI using a liquid chromatography tandem mass spectrometry method.
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  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%22Clinica+Chimica+Acta%22&quot;&gt;Clinica Chimica Acta&lt;/searchLink&gt;. Nov2022, Vol. 536, p162-168. 7p.
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– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Trimethylamine N -oxide (TMAO) and phenylacetylglutamine (PAGln) are associated with acute myocardial infarction (AMI) and type 2 diabetes mellitus (T2DM). This study developed and validated a simple method firstly for simultaneously quantifying serum TMAO and PAGln using liquid chromatography-tandem mass spectrometry (LC–MS/MS). Serum samples from patients with T2DM, AMI, and healthy subjects were analyzed using a new LC–MS/MS method to evaluate TMAO and PAGln levels. Statistical analyses were performed to evaluate TMAO and PAGln distributions among different groups. Retention and separation of the two metabolites were achieved within 5 min. For both analytes, the assay was linear in a 0.02–10 &#181;g/mL range, with &gt;0.99 average linear correlation coefficients, and quantification limit values of approximately 0.010 &#181;g/mL. The average recoveries of TMAO and PAGln were 96.3 % and 96.4 %, respectively. The intra-run and total coefficient variations were 3.5–4.8 % and 3.9–5.7 % respectively for TMAO; and 4.0–5.1 % and 4.6–6.3 % respectively for PAGln. TMAO and PAGln showed a moderate correlation (P &lt; 0.001) and their levels in patients with T2DM were significantly higher than those in healthy individuals (P &lt; 0.001). TMAO levels were higher in patients with T2DM than in patients with AMI (P &lt; 0.01). Patients with AMI had higher PAGln levels than healthy individuals (P &lt; 0.05). After adjusting for sex and age, the top tertile of PAGln was positively correlated with T2DM and AMI while that of TMAO was positively correlated with T2DM. Overall, a robust isotope dilution LC–MS/MS method was established, which may be beneficial for assessing the association between two metabolites with AMI and T2DM. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: &lt;i&gt;Copyright of Clinica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.cca.2022.09.018
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 7
        StartPage: 162
    Subjects:
      – SubjectFull: Liquid chromatography-mass spectrometry
        Type: general
      – SubjectFull: Bacterial metabolites
        Type: general
      – SubjectFull: Intestinal mucosa
        Type: general
      – SubjectFull: Type 2 diabetes
        Type: general
      – SubjectFull: Trimethylamine
        Type: general
      – SubjectFull: Myocardial infarction
        Type: general
    Titles:
      – TitleFull: Analysis of two intestinal bacterial metabolites (trimethylamine N-oxide and phenylacetylglutamine) in human serum samples of patients with T2DM and AMI using a liquid chromatography tandem mass spectrometry method.
        Type: main
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          Name:
            NameFull: Tang, Yueming
      – PersonEntity:
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            NameFull: Zou, Yutong
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            NameFull: Cui, Jingwen
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            NameFull: Ma, Xiaoli
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            NameFull: Zhang, Li
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            NameFull: Yu, Songlin
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            – D: 01
              M: 11
              Text: Nov2022
              Type: published
              Y: 2022
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              Value: 00098981
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              Value: 536
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            – TitleFull: Clinica Chimica Acta
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