Determination of chlorpromazine and its metabolites in animal-derived foods using QuEChERS-based extraction, EMR-Lipid cleanup, and UHPLC-Q-Orbitrap MS analysis.
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| Title: | Determination of chlorpromazine and its metabolites in animal-derived foods using QuEChERS-based extraction, EMR-Lipid cleanup, and UHPLC-Q-Orbitrap MS analysis. |
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| Authors: | Dai, Jinxia1 (AUTHOR), Lin, Hui2 (AUTHOR), Pan, Yuning2 (AUTHOR), Sun, Yanmin2 (AUTHOR), Wang, Ye1 (AUTHOR), Qiao, Jun-qin1 (AUTHOR), Lian, Hong-zhen1 (AUTHOR) hzlian@nju.edu.cn, Xu, Chun-xiang1,2 (AUTHOR) xcx70@163.com |
| Source: | Food Chemistry. Mar2023, Vol. 403, pN.PAG-N.PAG. 1p. |
| Subjects: | Chlorpromazine, Animal welfare, Mass spectrometry, Animal culture, Liquid chromatography |
| Abstract: | • A method to determine CPZ and its metabolites in animal-derived foods was proposed. • CPZ was prone to undergo hydrolytic transformation at high temperature. • The structures of hydrolysates of CPZ were clarified based on HRMS and software. • The method was suitably applied to analyze and screen CPZ-related risk compounds. Chlorpromazine (CPZ) is abused in animal husbandry and can be extensively metabolized in humans and animals. However, the actual monitoring mainly focuses on the parent compound but lacks attention to its metabolites. A method was developed and validated firstly for identification and determination of CPZ and its four major metabolites in animal-derived foods using ultrahigh-performance liquid chromatography coupled with quadrupole-Orbitrap mass spectrometry in combination with QuEChERS preparation method. Satisfactory recoveries of analytes spiked in fish and pork samples ranged from 72 to 117 %, and limits of quantification were 2.0 and 1.0 μg kg−1 for fish and pork samples respectively. Moreover, through the hydrolysis experiments of CPZ, its hydrolysates, such as CPZ-sulfoxide, CPZ- N -oxide and CPZ-sulfoxide- N -oxide, were identified as potential risk compounds. The developed method has been successfully applied to the determination of CPZ and its metabolites in actual commercial samples, as well as to the screening of other CPZ-related risk compounds. [ABSTRACT FROM AUTHOR] |
| Copyright of Food Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 160047864 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Determination of chlorpromazine and its metabolites in animal-derived foods using QuEChERS-based extraction, EMR-Lipid cleanup, and UHPLC-Q-Orbitrap MS analysis. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Dai%2C+Jinxia%22">Dai, Jinxia</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lin%2C+Hui%22">Lin, Hui</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pan%2C+Yuning%22">Pan, Yuning</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sun%2C+Yanmin%22">Sun, Yanmin</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Ye%22">Wang, Ye</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Qiao%2C+Jun-qin%22">Qiao, Jun-qin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lian%2C+Hong-zhen%22">Lian, Hong-zhen</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> hzlian@nju.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Xu%2C+Chun-xiang%22">Xu, Chun-xiang</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> xcx70@163.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Food+Chemistry%22">Food Chemistry</searchLink>. Mar2023, Vol. 403, pN.PAG-N.PAG. 1p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Chlorpromazine%22">Chlorpromazine</searchLink><br /><searchLink fieldCode="DE" term="%22Animal+welfare%22">Animal welfare</searchLink><br /><searchLink fieldCode="DE" term="%22Mass+spectrometry%22">Mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Animal+culture%22">Animal culture</searchLink><br /><searchLink fieldCode="DE" term="%22Liquid+chromatography%22">Liquid chromatography</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: • A method to determine CPZ and its metabolites in animal-derived foods was proposed. • CPZ was prone to undergo hydrolytic transformation at high temperature. • The structures of hydrolysates of CPZ were clarified based on HRMS and software. • The method was suitably applied to analyze and screen CPZ-related risk compounds. Chlorpromazine (CPZ) is abused in animal husbandry and can be extensively metabolized in humans and animals. However, the actual monitoring mainly focuses on the parent compound but lacks attention to its metabolites. A method was developed and validated firstly for identification and determination of CPZ and its four major metabolites in animal-derived foods using ultrahigh-performance liquid chromatography coupled with quadrupole-Orbitrap mass spectrometry in combination with QuEChERS preparation method. Satisfactory recoveries of analytes spiked in fish and pork samples ranged from 72 to 117 %, and limits of quantification were 2.0 and 1.0 μg kg−1 for fish and pork samples respectively. Moreover, through the hydrolysis experiments of CPZ, its hydrolysates, such as CPZ-sulfoxide, CPZ- N -oxide and CPZ-sulfoxide- N -oxide, were identified as potential risk compounds. The developed method has been successfully applied to the determination of CPZ and its metabolites in actual commercial samples, as well as to the screening of other CPZ-related risk compounds. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Food Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.foodchem.2022.134298 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: N.PAG Subjects: – SubjectFull: Chlorpromazine Type: general – SubjectFull: Animal welfare Type: general – SubjectFull: Mass spectrometry Type: general – SubjectFull: Animal culture Type: general – SubjectFull: Liquid chromatography Type: general Titles: – TitleFull: Determination of chlorpromazine and its metabolites in animal-derived foods using QuEChERS-based extraction, EMR-Lipid cleanup, and UHPLC-Q-Orbitrap MS analysis. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Dai, Jinxia – PersonEntity: Name: NameFull: Lin, Hui – PersonEntity: Name: NameFull: Pan, Yuning – PersonEntity: Name: NameFull: Sun, Yanmin – PersonEntity: Name: NameFull: Wang, Ye – PersonEntity: Name: NameFull: Qiao, Jun-qin – PersonEntity: Name: NameFull: Lian, Hong-zhen – PersonEntity: Name: NameFull: Xu, Chun-xiang IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2023 Type: published Y: 2023 Identifiers: – Type: issn-print Value: 03088146 Numbering: – Type: volume Value: 403 Titles: – TitleFull: Food Chemistry Type: main |
| ResultId | 1 |
