Rhodamine-Tagged Polymethacrylate Dyes as Alternative Tools for Analysis of Plant Cells.
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| Title: | Rhodamine-Tagged Polymethacrylate Dyes as Alternative Tools for Analysis of Plant Cells. |
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| Authors: | Bielas, Rafał1 (AUTHOR), Wróbel-Marek, Justyna2 (AUTHOR) justyna.wrobel@us.edu.pl, Kurczyńska, Ewa U.2 (AUTHOR), Neugebauer, Dorota1 (AUTHOR) justyna.wrobel@us.edu.pl |
| Source: | Materials (1996-1944). Nov2022, Vol. 15 Issue 21, p7720. 14p. |
| Subjects: | Polymethacrylates, Cell analysis, Organelles, Rhodamine B, Methacrylates, Cotyledons |
| Abstract: | A rhodamine B (RhB)-based initiator for atom transfer radical polymerization (ATRP) was synthesized and applied for preparation of poly(2-trimethylammoniumethyl methacrylate) (PChMA), poly(2-hydroxyethyl methacrylate) (PHEMA) and poly(2-trimethylsilyloxyethyl methacrylate) (PHEMATMS). Polymer fluorescence was confirmed by determination of quantum yield by comparative method with piroxicam as the standard exhibiting dependency of emission intensity on the polymer chain hydrophilicity and the kind of solvent. The RhB functionalized polymers were used for biological tests in plant materials except for RhB-PHEMATMS because of weak fluorescence. These two polymers slightly differed in cellular localization. RhB-PChMA was mostly observed in cell walls of root tissues and cotyledon epidermis. It was also observed in cytoplasm and cell organelles of root cap cells and rhizodermis, in contrast with cytoplasm of cotyledon epidermis. RhB-PHEMA was also present in apoplast. A strong signal in protoxylem cell walls and a weak signal in cell walls of rhizodermis and cortex were visible. Moreover, it was also present in cell walls of cotyledon epidermis. However, RhB-PHEMA was mostly observed in cytoplasm and cell organelles of all root tissues and epidermis of cotyledons. Both RhB-polymers did not cause cell death which means that they can be used in living plant material. [ABSTRACT FROM AUTHOR] |
| Copyright of Materials (1996-1944) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 160221340 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Rhodamine-Tagged Polymethacrylate Dyes as Alternative Tools for Analysis of Plant Cells. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Bielas%2C+Rafał%22">Bielas, Rafał</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wróbel-Marek%2C+Justyna%22">Wróbel-Marek, Justyna</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> justyna.wrobel@us.edu.pl</i><br /><searchLink fieldCode="AR" term="%22Kurczyńska%2C+Ewa+U%2E%22">Kurczyńska, Ewa U.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Neugebauer%2C+Dorota%22">Neugebauer, Dorota</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> justyna.wrobel@us.edu.pl</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Materials+%281996-1944%29%22">Materials (1996-1944)</searchLink>. Nov2022, Vol. 15 Issue 21, p7720. 14p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Polymethacrylates%22">Polymethacrylates</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+analysis%22">Cell analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Organelles%22">Organelles</searchLink><br /><searchLink fieldCode="DE" term="%22Rhodamine+B%22">Rhodamine B</searchLink><br /><searchLink fieldCode="DE" term="%22Methacrylates%22">Methacrylates</searchLink><br /><searchLink fieldCode="DE" term="%22Cotyledons%22">Cotyledons</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: A rhodamine B (RhB)-based initiator for atom transfer radical polymerization (ATRP) was synthesized and applied for preparation of poly(2-trimethylammoniumethyl methacrylate) (PChMA), poly(2-hydroxyethyl methacrylate) (PHEMA) and poly(2-trimethylsilyloxyethyl methacrylate) (PHEMATMS). Polymer fluorescence was confirmed by determination of quantum yield by comparative method with piroxicam as the standard exhibiting dependency of emission intensity on the polymer chain hydrophilicity and the kind of solvent. The RhB functionalized polymers were used for biological tests in plant materials except for RhB-PHEMATMS because of weak fluorescence. These two polymers slightly differed in cellular localization. RhB-PChMA was mostly observed in cell walls of root tissues and cotyledon epidermis. It was also observed in cytoplasm and cell organelles of root cap cells and rhizodermis, in contrast with cytoplasm of cotyledon epidermis. RhB-PHEMA was also present in apoplast. A strong signal in protoxylem cell walls and a weak signal in cell walls of rhizodermis and cortex were visible. Moreover, it was also present in cell walls of cotyledon epidermis. However, RhB-PHEMA was mostly observed in cytoplasm and cell organelles of all root tissues and epidermis of cotyledons. Both RhB-polymers did not cause cell death which means that they can be used in living plant material. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Materials (1996-1944) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3390/ma15217720 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 14 StartPage: 7720 Subjects: – SubjectFull: Polymethacrylates Type: general – SubjectFull: Cell analysis Type: general – SubjectFull: Organelles Type: general – SubjectFull: Rhodamine B Type: general – SubjectFull: Methacrylates Type: general – SubjectFull: Cotyledons Type: general Titles: – TitleFull: Rhodamine-Tagged Polymethacrylate Dyes as Alternative Tools for Analysis of Plant Cells. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Bielas, Rafał – PersonEntity: Name: NameFull: Wróbel-Marek, Justyna – PersonEntity: Name: NameFull: Kurczyńska, Ewa U. – PersonEntity: Name: NameFull: Neugebauer, Dorota IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 11 Text: Nov2022 Type: published Y: 2022 Identifiers: – Type: issn-print Value: 19961944 Numbering: – Type: volume Value: 15 – Type: issue Value: 21 Titles: – TitleFull: Materials (1996-1944) Type: main |
| ResultId | 1 |
