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Applications of bio-capacitance to cell culture manufacturing.

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Title: Applications of bio-capacitance to cell culture manufacturing.
Authors: Bergin, Adam1,2 (AUTHOR), Carvell, John1,3 (AUTHOR), Butler, Michael1,2 (AUTHOR) michael.butler@nibrt.ie
Source: Biotechnology Advances. Dec2022, Vol. 61, pN.PAG-N.PAG. 1p.
Subjects: Manufacturing cells, Cell culture, Trypan blue, Bioreactors, Biomass, Scalability
Abstract: This review focuses on why bio-capacitance has developed to become the standard online method to estimate biomass in cell-based bio-manufacturing processes. There is now a good understanding behind the apparent divergence seen between bio-capacitance and trypan blue based cell counts in the later stage of culture through recent works in the field of process monitoring. Methods for how various authors have been able to correct the bio-capacitance signal when required are discussed in this work. There is also a focus on the diversification and development of the probes for single use and smaller bioreactors. The review then describes the wide range of applications where bio-capacitance probes have been able to improve productivity. It includes on-line process control of perfusion-based processes, predictive feeding control of bioreactors, applications with attached cells and viral production. The work discusses the importance of scalability and the ability to validate the probes prior to manufacture and also reveals the additional process information that can be gleamed from the technology. [ABSTRACT FROM AUTHOR]
Copyright of Biotechnology Advances is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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IllustrationInfo
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  Data: Applications of bio-capacitance to cell culture manufacturing.
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  Data: <searchLink fieldCode="AR" term="%22Bergin%2C+Adam%22">Bergin, Adam</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Carvell%2C+John%22">Carvell, John</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Butler%2C+Michael%22">Butler, Michael</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> michael.butler@nibrt.ie</i>
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  Data: <searchLink fieldCode="JN" term="%22Biotechnology+Advances%22">Biotechnology Advances</searchLink>. Dec2022, Vol. 61, pN.PAG-N.PAG. 1p.
– Name: Subject
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  Data: <searchLink fieldCode="DE" term="%22Manufacturing+cells%22">Manufacturing cells</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+culture%22">Cell culture</searchLink><br /><searchLink fieldCode="DE" term="%22Trypan+blue%22">Trypan blue</searchLink><br /><searchLink fieldCode="DE" term="%22Bioreactors%22">Bioreactors</searchLink><br /><searchLink fieldCode="DE" term="%22Biomass%22">Biomass</searchLink><br /><searchLink fieldCode="DE" term="%22Scalability%22">Scalability</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: This review focuses on why bio-capacitance has developed to become the standard online method to estimate biomass in cell-based bio-manufacturing processes. There is now a good understanding behind the apparent divergence seen between bio-capacitance and trypan blue based cell counts in the later stage of culture through recent works in the field of process monitoring. Methods for how various authors have been able to correct the bio-capacitance signal when required are discussed in this work. There is also a focus on the diversification and development of the probes for single use and smaller bioreactors. The review then describes the wide range of applications where bio-capacitance probes have been able to improve productivity. It includes on-line process control of perfusion-based processes, predictive feeding control of bioreactors, applications with attached cells and viral production. The work discusses the importance of scalability and the ability to validate the probes prior to manufacture and also reveals the additional process information that can be gleamed from the technology. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Biotechnology Advances is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=160238417
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      – Type: doi
        Value: 10.1016/j.biotechadv.2022.108048
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      – Code: eng
        Text: English
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    Subjects:
      – SubjectFull: Manufacturing cells
        Type: general
      – SubjectFull: Cell culture
        Type: general
      – SubjectFull: Trypan blue
        Type: general
      – SubjectFull: Bioreactors
        Type: general
      – SubjectFull: Biomass
        Type: general
      – SubjectFull: Scalability
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      – TitleFull: Applications of bio-capacitance to cell culture manufacturing.
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            – D: 01
              M: 12
              Text: Dec2022
              Type: published
              Y: 2022
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