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Golden 2-like transcription factor contributes to the major QTL against rice black-streaked dwarf virus disease.

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Title: Golden 2-like transcription factor contributes to the major QTL against rice black-streaked dwarf virus disease.
Authors: Li, Xuejuan1,2 (AUTHOR), Lin, Feng1,3 (AUTHOR), Li, Chenyang1 (AUTHOR), Du, Linlin1 (AUTHOR), Liu, Zhiyang4 (AUTHOR), Shi, Wenjuan1,2 (AUTHOR), Lv, Jianying1,2 (AUTHOR), Cao, Xiaoyan1,2 (AUTHOR), Lan, Ying1 (AUTHOR), Fan, Yongjian1 (AUTHOR), Zhou, Yijun1 (AUTHOR), Zhou, Tong1,2,3 (AUTHOR) zhoutong@jaas.ac.cn
Source: Theoretical & Applied Genetics. Dec2022, Vol. 135 Issue 12, p4233-4243. 11p.
Subjects: Virus diseases, Rice, Laodelphax striatellus, Transcription factors, Wheat diseases & pests, Amino acid sequence
Geographic Terms: East Asia, China
Abstract: Key message: A major resistance QTL was identified on chromosome 6 in rice variety Wuke; both overexpression and knockdown experiments confirmed that OsGLK1 is the candidate gene for association with Rice black-streaked dwarf virus disease. Rice black-streaked dwarf virus disease is one of the most destructive rice viral diseases in China and East Asia. Progress has been limited in RBSDVD resistance breeding due to inadequate knowledge on the underlying functional genes. In this study, a major QTL for RBSDV (rice black-streaked dwarf virus) independent of SBPH (small brown planthopper) resistance was mapped in a 1.8 Mb interval on chromosome 6 by using an F2:3 population originated from resistant rice variety Wuke. Representative transcripts within this region were analysed and three genes showing amino acid sequence variation in functional domains were selected for transformation. Overexpression experiments showed that one gene exhibited significant enhanced resistance compared to control lines, encoding protein involving Myb domain and probable transcription factor Golden 2-like1 (GLK1). Furthermore, OsGLK1 knockdown rice lines were investigated and the resistance ability was significantly declined without this gene compared to the wild type. Taken together, both overexpression and knockdown experiments strongly suggested that OsGLK1 plays an important role for RBSDV resistance and contributes to the major QTL. The study paves the way for elucidating the molecular mechanism underlying RBSDVD resistance and the molecular markers associated with OsGLK1 may be used for marker-assisted selection. [ABSTRACT FROM AUTHOR]
Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Golden 2-like transcription factor contributes to the major QTL against rice black-streaked dwarf virus disease.
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  Data: <searchLink fieldCode="AR" term="%22Li%2C+Xuejuan%22">Li, Xuejuan</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lin%2C+Feng%22">Lin, Feng</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Chenyang%22">Li, Chenyang</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Du%2C+Linlin%22">Du, Linlin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Zhiyang%22">Liu, Zhiyang</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Shi%2C+Wenjuan%22">Shi, Wenjuan</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lv%2C+Jianying%22">Lv, Jianying</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cao%2C+Xiaoyan%22">Cao, Xiaoyan</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lan%2C+Ying%22">Lan, Ying</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Fan%2C+Yongjian%22">Fan, Yongjian</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhou%2C+Yijun%22">Zhou, Yijun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhou%2C+Tong%22">Zhou, Tong</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<i> zhoutong@jaas.ac.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. Dec2022, Vol. 135 Issue 12, p4233-4243. 11p.
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  Data: <searchLink fieldCode="DE" term="%22Virus+diseases%22">Virus diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Rice%22">Rice</searchLink><br /><searchLink fieldCode="DE" term="%22Laodelphax+striatellus%22">Laodelphax striatellus</searchLink><br /><searchLink fieldCode="DE" term="%22Transcription+factors%22">Transcription factors</searchLink><br /><searchLink fieldCode="DE" term="%22Wheat+diseases+%26+pests%22">Wheat diseases & pests</searchLink><br /><searchLink fieldCode="DE" term="%22Amino+acid+sequence%22">Amino acid sequence</searchLink>
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  Data: <searchLink fieldCode="DE" term="%22East+Asia%22">East Asia</searchLink><br /><searchLink fieldCode="DE" term="%22China%22">China</searchLink>
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  Label: Abstract
  Group: Ab
  Data: Key message: A major resistance QTL was identified on chromosome 6 in rice variety Wuke; both overexpression and knockdown experiments confirmed that OsGLK1 is the candidate gene for association with Rice black-streaked dwarf virus disease. Rice black-streaked dwarf virus disease is one of the most destructive rice viral diseases in China and East Asia. Progress has been limited in RBSDVD resistance breeding due to inadequate knowledge on the underlying functional genes. In this study, a major QTL for RBSDV (rice black-streaked dwarf virus) independent of SBPH (small brown planthopper) resistance was mapped in a 1.8 Mb interval on chromosome 6 by using an F2:3 population originated from resistant rice variety Wuke. Representative transcripts within this region were analysed and three genes showing amino acid sequence variation in functional domains were selected for transformation. Overexpression experiments showed that one gene exhibited significant enhanced resistance compared to control lines, encoding protein involving Myb domain and probable transcription factor Golden 2-like1 (GLK1). Furthermore, OsGLK1 knockdown rice lines were investigated and the resistance ability was significantly declined without this gene compared to the wild type. Taken together, both overexpression and knockdown experiments strongly suggested that OsGLK1 plays an important role for RBSDV resistance and contributes to the major QTL. The study paves the way for elucidating the molecular mechanism underlying RBSDVD resistance and the molecular markers associated with OsGLK1 may be used for marker-assisted selection. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1007/s00122-022-04214-9
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      – Code: eng
        Text: English
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        PageCount: 11
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      – SubjectFull: Virus diseases
        Type: general
      – SubjectFull: Rice
        Type: general
      – SubjectFull: Laodelphax striatellus
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      – SubjectFull: Amino acid sequence
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      – SubjectFull: East Asia
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              Text: Dec2022
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